ARTICLES

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ABSTRACT

China Factory Offer New Core I7 15.6 Laptop. After enzymatic hydrolysis,Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates.Mining Machinery Parts Diamond Saw Blade Segments for Marble. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding;China Plastic Grid for Paver Grass for Gardening; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Explosion Proof Rechargeable LED Searchlight, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1, 1996;Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Soft Rubber Hair Remover Pet Brush,1991).Forging Metal Building Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound,Safety Traffic Rubber Roof Support Feet Products,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring(Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1, 1996). The soluble protein may have commercial use as gelatines,Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology, peptides or amino acids production (Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter, 1991;Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rubber Tire Plastic Recycling Shredder with Factory Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft.Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Stainless Steel 304 Wire Rope Fork Terminal(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle).Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet⁰C for 24 h. The sample was then treated with 1%Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)⁰C for 2 h. After this treatment,CAS 85-44 9 Flakes Phthali Anhydride. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator, as shown inRutile Anatase Titanium Dioxide for Coating and Paints.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Blue Silica Gel with Indicating, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

33kv Electrical Automatic Voltage Regulator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminum Coil with AA3003 AA3105 H14 H16 H18) for the financial support.

REFERENCES

4 Gang 1 Way Electrical Fitting Ce Certificate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pvc Pipe Injection Moulding Machine]

Brown, E. M; Thompson, C .J.,High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw, M.M.(1994),Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6.The Journal of the American Leather Chemists Association (JALCA)89(7)[Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine. Analytical Biochemistry212,436-445[CNC Turning Brass Part for Power Cable Connector]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-SGS 40L Lab Use Chocolate Refiner Conching Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Nickel 200 Floating Ball Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Samsung Pick and Place Machine for LED Component Mounting]

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Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Flat Pressure Indentation Punching Machine Automatic Card Box Die Cutting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rapid and Efficient Cooperation Multi Color Ribbon for Bows]

Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken]

Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hand Push Thermoplastic Paint Pedestrians Line Road Marking Machine Price, Part I.50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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