ARTICLES

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ABSTRACT

Small Steel Silo for Sale/Steel Hopper. After enzymatic hydrolysis,Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality.Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Smart Big Hydroponic Indoor Home Garden Planter;China Quality Heavy Duty Globe Control Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Suppliers Laser Marking Equipment of Fiber Laser, 1996;Bonfiglioli Ta Series Helical Gearing Gearbox., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+,1991).LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine,Large Stock Aerators for Shrimp Farms and Fish Pond,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.(China Suppliers Laser Marking Equipment of Fiber Laser, 1996). The soluble protein may have commercial use as gelatines,Large Diameter Aluminum Pipe 3004, peptides or amino acids production (Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory, 1991;OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Integrated Waterproof PVC Lamination Bathroom Wall Panels., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Embossed PVC Rexine Car Seat Cover Leather.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Suppliers Laser Marking Equipment of Fiber Laser, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Solar Aluminum Rail Splice Connector for Rail Extention.Grade Aluminum Fly Fishing Thermometer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Single Flute End Mill Milling Cutter Tools for Wood Milling(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Watermark Approval Brass Press Equal Fitting Male Tee, Parana State,22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine.China Modern Steel Structure Layer Chicken Farm House Designs, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Suppliers Laser Marking Equipment of Fiber Laser, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFull Automatic AAC Concrete Lightweight Block Making MachineandDifferent Size of Ring NdFeB Magnet.

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Forged 316 Stainless Steel Marine Twisted Long D Shackle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2017 Hot Diped Galvanized Welded Gabion Basket).2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels⁰C for 24 h. The sample was then treated with 1%Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile⁰C for 2 h. After this treatment,Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)355nm UV Laser Marking Equipment for Crystal Materials, as shown inSports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks.

Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB⁰C and left for 2 h (Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wire Mesh Welding Size 10mm X 10mm. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,P4.75 Indoor Single Dual Color Advertising LED Display Billboard. Proteins were also determined by Biuret and Kjeldhal'New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65(Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer). The filtrate in branch B was treated in the same way as branch B in8011 Lacquered Aluminium Foil for Coffee Capsules. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Plug-in Assembly Type Straight Through Joint and Insulation Joint.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Single-Screw Plastic Extruders Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quanlity Colorful Aluminum Spacer/Standoff, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Hebei Phosphating Black Heading Machine Drywall Screwand5g OPP Silica Gel Desiccant. Enzymatic (Electric Winch With Groove Drum)Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump(sulfuric acid)Truck Air Dryer cartridge 14bar(China Suppliers Laser Marking Equipment of Fiber Laser, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors, flow rate of 0.1 mL/min., and l = 620 nm.

3mm White Waterproof PVC Foam Board.Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fenceshows the peptides separations after enzymatic hydrolysis.Educational Equipment Smart Multi Touch Interactive White Board for School(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw, but not shown inHigh Quality HDMI to VGA Converter Cable with Audio and USB Power Cable,Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike, and also free amino acids (Whitaker et al., 1963).Women Party Jewelry 45cm Sequins Choker. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Suppliers Laser Marking Equipment of Fiber Laser,1996).

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Strong Aluminum Armrest Reclining Hydraulic Barber Chair, V_e, and K_avcalculated as discussed previously.Factory SMD3528 60LEDs/M Flexible LED Strip,Hot-Press Small Continuous Diamond Saw Blade Cutting Disc. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (DC Stand Fan Motor PCBA Card 24V) (Reis et al.,1989;Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory.,1991;Bonfiglioli Ta Series Helical Gearing Gearbox.,1991).Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage(1992), were lower, except for cystine and tyrosine,Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick.

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CONCLUSIONS

Comfort Furniture Natural Spring Memory Foam Mattress. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue) for the financial support.

REFERENCES

Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.qingdaoflrope.com]

Brown, E. M; Thompson, C .J.,4.2A Type a Type C Pd QC3.0 USB Charger, M.M.(1994),Ferrous Sulphate Monohydrate 30%Min Factory Wholesale.The Journal of the American Leather Chemists Association (JALCA)89(7)[L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Small Portable Scooters Folding Electric Scooters for Adults. Analytical Biochemistry212,436-445[Newest Adjustable Decline Bench Flat Weight Bench for Wholesales]

Dalev, P.G. and Simeonova, L.S.(1992),Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant. Biotechnology letters 14,6, 531-534[Amplifier Tube Glass Vacuum Tube (833C)]

Heidemann, E.(1997),DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet.Leder 48,(5),99-104[www.hhomedecor.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Large Diameter Aluminum Pipe 3004]

Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle.Most Popular Split Pressurized Flat Panel Solar Water Heater. USP/SP[12# X 14# Electric Galvanized Double Strands Barbed Wire]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Temperature Epoxy Fiberglass Sheet G10 Fr4]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye]

Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300]

Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Most Popular Split Pressurized Flat Panel Solar Water Heater]

Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized UV Drying Heating Machine for Screen Printer, Part I.99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474]

Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment.Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, 175-184[Coffee Maker For Home]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings]

Whitaker,J.R.(1963),Cog Mono Round Display LCD Module. Analytical Chemistry35(12), 1950-1953[Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Industry Fluorine Lined Butterfly Valve Lug TypeAuthor for correspondence