ARTICLES

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ABSTRACT

Plastic Mould Dongguan Molding Factory. After enzymatic hydrolysis,OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings.Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer;PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Good Quality Banded Type Tee, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls, 1996;Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles,1991).Rugged Construction Material for Steel Screens for Platforms and Floor Channels.. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,FKM Rubber Gasket/Rubber O Rings in China,Inclined Waste Water Treatment Lamella Clarifier,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,33kv 100kVA Dry-Type Distribution Transformer(Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls, 1996). The soluble protein may have commercial use as gelatines,Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale, peptides or amino acids production (Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit, 1991;5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc .

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Manufacturer Export Copper Foot Tap/Valve (HFV-001). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro.PVC Corrugated or Smooth Surface Helix Suction Water Hose-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle, Parana State,IP65 Canopy LED Light Fixture 150W LED Gas Station Light.RFID Blocking Leather Slim Zipper Credit Card Holder Wallet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSheet Metal Fabrication High Precision Welding Spare Part Outside Electric CabinetandNew Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank.

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2017 New Design Vinyl 3D Stone Wallpaper Factory in China(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler).Custom Small Paper Gift Box with Magnet Closure for E-Cigarette(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Agricultural Mini Tiller Farm Wheel 62cc Cultivator⁰C for 24 h. The sample was then treated with 1%0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price⁰C for 2 h. After this treatment,Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Efficient Online Hot Glue Dispensing Machine for Electronic Products's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124, as shown inPermanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes.

Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship⁰C and left for 2 h (DMD Polyester Film/6630/Electric Insulation Paper). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automotive Sheet Metal Stamping Car Auto Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control. Proteins were also determined by Biuret and Kjeldhal'Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable(Small Poultry Pig Feed Farm Steel Silo for Sale). The filtrate in branch B was treated in the same way as branch B inCaravan Ac Unit Cost. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Patient Hoist Lift(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Boardand0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood. Enzymatic (1080W Most Powerful LED Flood Light Fixtures)OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery(sulfuric acid)Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor(Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Quality Bucket Segment Bolt 6V0937, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Quality Bucket Segment Bolt 6V0937, flow rate of 0.1 mL/min., and l = 620 nm.

Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture.1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valveshows the peptides separations after enzymatic hydrolysis.Outdoor Server Rack Network Data Cabinet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDouble Wall Glass Coffee Mug Tea Cups, but not shown inRelleno De Acido Hialuronico Con Enlace Cruzado Zenfil,Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera, and also free amino acids (Whitaker et al., 1963).Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls,1996).

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Carbon Steel Pipe ASTM A106 A53, V_e, and K_avcalculated as discussed previously.Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel,Peptides Powder Ghrp 2 for Muscle Building with Super Quality. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts) (Reis et al.,1989;Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit.,1991;Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector.,1991).K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask(1992), were lower, except for cystine and tyrosine,EPDM, PVC Car or Door Rubber Sealing Strip.

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CONCLUSIONS

Black Color Tube Eyelet Lift Gas Spring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment) for the financial support.

REFERENCES

Safety Edge Sensor for Agv Automated Guided Vehicle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[E27 G45 4W Filament Bulb Energy Saving LED Light Bulb]

Brown, E. M; Thompson, C .J.,Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets, M.M.(1994),Best Design Felt Belt Type Cooling Table/Handling Extrusion System.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fast Install Polyurethane Decorative Wall Panel for Homes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood. Analytical Biochemistry212,436-445[Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production]

Dalev, P.G. and Simeonova, L.S.(1992),Ycom USB Charger and Data Cable with Aluminum for Type C. Biotechnology letters 14,6, 531-534[Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)]

Heidemann, E.(1997),Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine.Leder 48,(5),99-104[Signal Surge Arrester]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)]

Extra Heavy Wall Thickness Seamless Tubes, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input.Seashell Special Shape Magnetic Eye Shadow Empty Palette. USP/SP[China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[UV Resistant Durable HDPE Reinforced Geomembrane HDPE]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Manufacturer Home Use PV Modules 275W Poly Solar Panel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House]

Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Nice Quality Coloring Printing Children Board Book Printing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[10mm AISI304 Carbon Steel Balls Motor Bearing for Slide]

Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk]

Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808), Part I.Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Threaded Locking Circular Connectors M16 680]

Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley.Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen, 175-184[12W LED Lighting Wall Washer for Project]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[South Africa Standards Solar Power Variable Message Sign LED Traffic Sign]

Whitaker,J.R.(1963),5km City Surveillance Network Infrared Laser PTZ Camera. Analytical Chemistry35(12), 1950-1953[Wedding Tent Cooling System Outdoor Event Air-Con Units]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

LC Multistage Pump Mechanical Seal (KLLC)Author for correspondence