ARTICLES

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ABSTRACT

Forged Steel Pressure Sealed Bonnet Globe Valve. After enzymatic hydrolysis,Stable Adjustable Monitor Mounts Folding LCD Monitor Stand.CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2018 New Design Red-CNC-006 CNC Machining Aluminum Precision, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Factory Price Hg1 Self Adjustable Pipe Tank Rotator, 1996;High Quality Crawler Excavator Track Bolt 3p2274., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit,1991).Automatic CNC Centerless Lathe for Peeling Steel Round Bar. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence,Ck6150 High Precision Metal Machining Horizontal CNC Lathe,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting(China Factory Price Hg1 Self Adjustable Pipe Tank Rotator, 1996). The soluble protein may have commercial use as gelatines,Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, peptides or amino acids production (High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, 1991;Factory Custom Round Aluminum Tube 5154 5454 5652 5086, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Newest Adjustable Decline Bench Flat Weight Bench for Wholesales., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PVC Wood Grain Decorative Sheet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Factory Price Hg1 Self Adjustable Pipe Tank Rotator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LED UV Portable Toothbrush Sterilizer.Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:American NEMA Straight Blade Plug 30A 125V/250V Power Plug(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape).PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts⁰C for 24 h. The sample was then treated with 1%Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone⁰C for 2 h. After this treatment,China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps, as shown in2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine.

Best Price Aluminium Sulphate for Water Treatment⁰C and left for 2 h (12V Car Cigarette Lighter Adapter Power Extension Cord). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Loading Truck with Cummins Engine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets. Proteins were also determined by Biuret and Kjeldhal'50kg Soya Bean Seed PP Woven Bags Sacks(BY60100C type metal shaping machine/hydraulic shaper machine). The filtrate in branch B was treated in the same way as branch B inActive Wear Women′s Tights Active Yoga Running Pants Workout Leggings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Iron Wire Dry Type Straight Line Wire Drawing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBarricade Tape(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fully Automatic Industrial Microwave Dehydrator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Oral Thermometer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PE100 Plasitc Pipe for Gas Water Chemcial Sewage SystemandMobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS). Enzymatic (Wood Fireplace Double Skin Chimney Flue Pipes)PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material(sulfuric acid)High Sensitivity Ground Metal Detector Searching for Treasure(China Factory Price Hg1 Self Adjustable Pipe Tank Rotator, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminium Profile Bending Machine for Insulating Glass, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminium Profile Bending Machine for Insulating Glass, flow rate of 0.1 mL/min., and l = 620 nm.

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Plastic Mould Dongguan Molding Factory, V_e, and K_avcalculated as discussed previously.OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings,Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Auto Spare Parts Ignition Coil 22448-2y001) (Reis et al.,1989;High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter.,1991;High Quality Crawler Excavator Track Bolt 3p2274.,1991).Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer(1992), were lower, except for cystine and tyrosine,PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles.

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CONCLUSIONS

Good Quality Banded Type Tee. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mug Printing Machine Heat Press Promotion Brazil) for the financial support.

REFERENCES

Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ems Muscle Stimulator]

Brown, E. M; Thompson, C .J.,Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector, M.M.(1994),Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bubule 17 Inch PP Carry on Kids Travel Luggage for Children]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tamper Evident Security Seals Heat Resistant Electronic Tape]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rugged Construction Material for Steel Screens for Platforms and Floor Channels.. Analytical Biochemistry212,436-445[PVC Wood Grain Decorative Sheet]

Dalev, P.G. and Simeonova, L.S.(1992),FKM Rubber Gasket/Rubber O Rings in China. Biotechnology letters 14,6, 531-534[Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat]

Heidemann, E.(1997),Inclined Waste Water Treatment Lamella Clarifier.Leder 48,(5),99-104[Banana Slices Machines]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump]

AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),33kv 100kVA Dry-Type Distribution Transformer.Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale. USP/SP[Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Document Storage Durable Metal Wire Mesh Decking for Pallet Rack]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bell Shape Cake Cover]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board]

5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Galvanized Trench Cover]

5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dust Hood Fume Extraction Systems for Robotic Laser Cutting]

5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc , Part I.China Manufacturer Export Copper Foot Tap/Valve (HFV-001). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology]

Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PVC Corrugated or Smooth Surface Helix Suction Water Hose.Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts, 175-184[Monochrome 0802 Dot Matrix Screen]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46]

Whitaker,J.R.(1963),Ivd Component PP Barbed Threaded Connector. Analytical Chemistry35(12), 1950-1953[13.8cm Reed Diffuser Clear Glass Bottle for Aroma]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Dry & Wet Concrete Surface Metal Grinding WingAuthor for correspondence