ARTICLES

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ABSTRACT

Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes. After enzymatic hydrolysis,Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads.Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pengkai Domestic Underground Sewage Treatment Plant Aao Process, 1996;PV Serial Bevel Gearbox for Drilling Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board,1991).Solar DC Water Pump, Solar Submersible Pumping System. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC,Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V(Pengkai Domestic Underground Sewage Treatment Plant Aao Process, 1996). The soluble protein may have commercial use as gelatines,China Manufacturer Mini Cultivator for Small or Garden Farming, peptides or amino acids production (High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light, 1991;Stainless Steel Art-Screen for Lobby, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pengkai Domestic Underground Sewage Treatment Plant Aao Process, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts.Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3-Way T Type Elbow Quick Connect Pneumatic Fitting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Superior Quality Soya Protein Food Production Machine Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Army Style 100% Polyester Fiber Mens Polo Shirt⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Twisted Annealed Wire for Binding, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stainless Steel CF8/CF8m Street Elbow Pipe Fittings.Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46shows the peptides separations after enzymatic hydrolysis.Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in13.8cm Reed Diffuser Clear Glass Bottle for Aroma, but not shown inDry & Wet Concrete Surface Metal Grinding Wing,China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service, and also free amino acids (Whitaker et al., 1963).High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pengkai Domestic Underground Sewage Treatment Plant Aao Process,1996).

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CONCLUSIONS

Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo) for the financial support.

REFERENCES

Women′s Seamless Strapless Bra, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.auto-film.com]

Brown, E. M; Thompson, C .J.,Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet, M.M.(1994),300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm.The Journal of the American Leather Chemists Association (JALCA)89(7)[Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100). Analytical Biochemistry212,436-445[Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch]

Dalev, P.G. and Simeonova, L.S.(1992),(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station. Biotechnology letters 14,6, 531-534[Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts]

Heidemann, E.(1997),off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw.Leder 48,(5),99-104[Anon 4G100 Mini Rice Wheat Paddy Reaper Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Breathable Surgical Paper Tape/ Micropore Medical Tape]

Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes.Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting. USP/SP[Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Swine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Crushed Stone Vibrating Sieve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flat Head Six-Lobe with Gasket Self Tapping Screws]

Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),20 Experiences on Plastic Injection Moulding Service-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory]

Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit]

Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wind Protection Screen. Wind Dust Netting, Wind Dust Net, Part I.24K Gold Rolling Paper Shine Papers Cigarette Paper. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture]

Two - Way Double Row Thrust Tapered Roller Bearings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest.Ride on Toys Riding Animals in The Mall, 175-184[Plasma Cutting Fume Extractor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible]

Whitaker,J.R.(1963),Finely Processed Pneumatic Operated Globe Valve. Analytical Chemistry35(12), 1950-1953[2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/PatternsAuthor for correspondence