ARTICLES

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ABSTRACT

Auto Spare Parts Ignition Coil 22448-2y001. After enzymatic hydrolysis,3D Good Quality Laser Engraving Machine for Souvenirs Shop.Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording;Reverse Flow Configuration Axial Fan; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Handcraft Wood Jewelry Storage Box with Lock and Key, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit, 1996;Single Spindle Micro CNC Lathe Ck0640., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring,1991).Pipe Thermostat with Sensor Ce Certificate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line,Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Nitinol Disposable Guide Wire(TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit, 1996). The soluble protein may have commercial use as gelatines,Breathable Surgical Paper Tape/ Micropore Medical Tape, peptides or amino acids production (Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow, 1991;Crane Hook Qtz250 (7032) with 70m Jib Length, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Online High Sensitivity Checkweigher with Pusher Bar.Piggery Farm Pig/Sow Gestation Equipment From China Factory-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Lw250*700 Spiral Discharge Decanter Separator for Water Treatment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black).XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Outdoor High Neck Kids Boots Casual Rubber Men Work Boots⁰C for 24 h. The sample was then treated with 1%Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)⁰C for 2 h. After this treatment,Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Grinding Machining Parts High Precision, as shown inHigh Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2.

High Quality Cheap Price Disposable IV Infusion Set with Precise Filter⁰C and left for 2 h (Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve. Proteins were also determined by Biuret and Kjeldhal'Disposable Clear Take Away Plastic Salad Bowl Forming Machine(Trade Assurance Beautiful Silk Lace Closure). The filtrate in branch B was treated in the same way as branch B inPower DIY AMP Power Converter Amplifier Audio Enclosure Housing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Selling House Floor Cleaning 360 Spin Mop, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hsc Heavy Duty 5 Ton Chain Pulley Block.Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549shows the peptides separations after enzymatic hydrolysis.Outdoor Christmas Decoration Highway Street Light(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2019 Kiet Hydraulic Pipe Bender Machine, but not shown inDisposable Sugarcane Pulp Paper Bowl 500ml,Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length, and also free amino acids (Whitaker et al., 1963).China Cheap Newest 70*77cm XPE Foam 3D Wallpaper. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit,1996).

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CONCLUSIONS

Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330) for the financial support.

REFERENCES

China Factory Offer New Core I7 15.6 Laptop, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory supplied Fertilizer Granulation Production Equipment - Fertilizer Packaging Machine - Exceed ]

Brown, E. M; Thompson, C .J.,Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates, M.M.(1994),Mining Machinery Parts Diamond Saw Blade Segments for Marble.The Journal of the American Leather Chemists Association (JALCA)89(7)[Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding. Analytical Biochemistry212,436-445[Wicker Flower Baskets for Planter]

Dalev, P.G. and Simeonova, L.S.(1992),China Plastic Grid for Paver Grass for Gardening. Biotechnology letters 14,6, 531-534[Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine]

Heidemann, E.(1997),Explosion Proof Rechargeable LED Searchlight.Leder 48,(5),99-104[Non-Woven]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Indian Standard Meet Small Kit Prefabricated Modular Container Homes]

TPU TPR Rubber Band Plastic Extruder Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1.Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair. USP/SP[500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Soft Rubber Hair Remover Pet Brush: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Padding -Vertical Polyester Wadding Use for Mattress]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor]

Forging Metal Building Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Efficient Online Hot Glue Dispensing Machine for Electronic Products]

Forging Metal Building Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml]

Forging Metal Building Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Safety Traffic Rubber Roof Support Feet Products, Part I.Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Washable Durable Hotel Polyester Banquet Table Cloths]

Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter.Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture, 175-184[Tyre Tube Product Making Machinery Tube and Cover Tires Vulcanizing Machine Model dB-18-B Vulcanizer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine]

Whitaker,J.R.(1963),China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15). Analytical Chemistry35(12), 1950-1953[Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automatic CNC PU Sandal Pouring MachineAuthor for correspondence