ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-316L 10 Micron Sintered Stainless Steel Filter Disc. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket

ABSTRACT

Abstract Light and Shadow Metal Oil Painting for Deco. After enzymatic hydrolysis,Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003).Cheapest Container Living House/Office/Kiosk. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Spin Centrifugal Demister Separator or Column Internal;Baby Wet Wipe Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric, 1996;360 Degree Reciprocating Car Wash Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Temperature Sensor Customized Thermostat for Industrial Appliances,1991).Custom Die Aluminum Castings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Meat Pie Making Machine with Different Mould Shape,Gear Operated Flanged High Performance Butterfly Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck(Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3, peptides or amino acids production (Wholesale Universal Garden Smart Flower Pot, 1991;Reflective Tape for Vehicle Reflective Markings (tape-02), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;White Crystal Powder Betaine Anhydrous CAS#107-43-7., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Creative New Product Coin Holder Keychain.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Weak Daylight Recharge All in One Solar Street Light.Ybz16-150/140 Vane Steering Hydraulic Pump-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Polypropylene Fibre for Commercial Slabs(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings).Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Glass Water Jet Cutting Machine (Skwj-4018A)⁰C for 24 h. The sample was then treated with 1%U. S. Type Clevis Grab Hook⁰C for 2 h. After this treatment,Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Car Trunk Organizer Car Storage Organizer, as shown inExterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights.

Fashion Cotton Baby/Kid Hat (DH-BF332)⁰C and left for 2 h (Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,OEM Round Stainless Steel Fruit Plate Tray. Proteins were also determined by Biuret and Kjeldhal'Factory Supply Industry Grade Sodium Gluconate(New Fashion Diaper Bags Mummy Bag). The filtrate in branch B was treated in the same way as branch B inHot Sale Aluminum Cheap Mobile Stage Truss for Concert. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMini High Temperature Clothing Labels Screen Printing Machine Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Conventional Best-Selling Household Bamboo Craft Chopsticks, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Electro Galvanized Low Carbon Straight Cut Wire.Classic Black Color Commercial Glass Door Multi Deck Refrigeratorshows the peptides separations after enzymatic hydrolysis.Geotextile for Roofs, Civil Applications. Railway Work(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPlastic Recycling Centrifugal Dewatering Machine, but not shown inMosquito Proof Fiberglass Fly Screen,Pneumatic Shuttle Valve Flange Ends, and also free amino acids (Whitaker et al., 1963).Stx825 Dual 15 Powerful PA Speaker, Speaker Box. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric,1996).

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CONCLUSIONS

Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic CNC PU Sandal Pouring Machine) for the financial support.

REFERENCES

SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chain Link Fence Machine Exporter Hot Sale Product]

Brown, E. M; Thompson, C .J.,Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production, M.M.(1994),110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tractor Tine Tiller Cultivator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Supply 20-110mm PP Reducing Tee for Pipe Systems. Analytical Biochemistry212,436-445[Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides]

Dalev, P.G. and Simeonova, L.S.(1992),5 Gallon Pure Water Bottle Non-Spill Cap. Biotechnology letters 14,6, 531-534[5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring]

Heidemann, E.(1997),Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks.Leder 48,(5),99-104[m.acrubberfactory.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Car Trunk Organizer Car Storage Organizer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Small Capacity Automatic Cup Filling Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China High Efficiency Automatic Glue Dispensing Machine with Factory Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Jcb Spare Parts for Gasket 813/00466]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning]

ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont]

ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring]

ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Small Capacity Automatic Cup Filling Machine, Part I.Seasoned Cast Iron South Africa Three Legged Potjie Pot. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Aluminum Carpet Topping Stage Platform]

Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine.2019 New Release Doypack Sachet Powder Packing Machine Price, 175-184[Hc-05 Subtitute Wireless Bluetooth RF Serial Transceiver Module 6pin RS232]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professtional Suppliers Yutong Golden Dragon Bus Spare Parts]

Whitaker,J.R.(1963),Amplifier Tube Glass Vacuum Tube (833C). Analytical Chemistry35(12), 1950-1953[Economy Combo Heat Press for Starter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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