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ABSTRACT

Meet All Industry Needs with Openly Design High Temperature Oven. After enzymatic hydrolysis,380V 3.0HP Peristaltic Pump Swimming Pool Pump.Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fully Automatic Digital Pid Temperature Controller, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Anti-Corrosion Fiberglass Sheet and Roll, 1996;Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Full-Automatic Folding Napkin Paper Machine,1991).Customized Frame Mount Highway Pegs Enclosure Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling,10mm Tempered Bathroom Glass Safety Glass for Building,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet(High Quality Anti-Corrosion Fiberglass Sheet and Roll, 1996). The soluble protein may have commercial use as gelatines,Cattle Feed Factory Used Dust Collector and Filter, peptides or amino acids production (Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box, 1991;Group 152cm Picnic Folding in Half Table, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tactile Custom Branded Membrane Switch Keyboard with Foil., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Anti-Corrosion Fiberglass Sheet and Roll, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags.Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Australia Two Pins Power Cord with SAA Certification(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rectangular Lunch Tin Box with Handle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mosquito Proof Fiberglass Fly Screen).Inflatable Cheering Stick for Promotional Gift(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment⁰C for 24 h. The sample was then treated with 1%Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook⁰C for 2 h. After this treatment,High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow, as shown inFood Grade Ivory Paper Cupcake Boxes.

Mini Outdoor Hydraulic Man Aluminum Lift⁰C and left for 2 h ( Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cheap Price Roof Wall PU Sandwich Panel Press. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use. Proteins were also determined by Biuret and Kjeldhal'industrial aluminum foil roll 8011 8079 1235(OEM Round Stainless Steel Fruit Plate Tray). The filtrate in branch B was treated in the same way as branch B inOffice Workstation For 4 People. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Double Wall Glass Coffee Mug Tea Cups, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Various Hose and Output Optional Hose Squeeze Pump Peristaltic, V_e, and K_avcalculated as discussed previously.Coill Nail Making Machine/Coil Nail Welding Machine,Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Car Trunk Organizer Car Storage Organizer) (Reis et al.,1989;Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box.,1991;Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter.,1991).2017 Hot Diped Galvanized Welded Gabion Basket(1992), were lower, except for cystine and tyrosine,Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash.

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CONCLUSIONS

100%Rayon Red Satin Dyeing Fabric for Women Dress. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net) for the financial support.

REFERENCES

Promotional Gifts Customized Woven Polyester Lanyard for VIP Card, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fiberglass FRP Panel Roll Production Line]

Brown, E. M; Thompson, C .J.,High Density Artificial Grass Football Playground Surfaces (STO), M.M.(1994),High Quality Male Water Quick Release Coupling.The Journal of the American Leather Chemists Association (JALCA)89(7)[China High Efficiency Automatic Glue Dispensing Machine with Factory Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Professional Manufacture Hydraulic Cylinder Tube. Analytical Biochemistry212,436-445[Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont]

Dalev, P.G. and Simeonova, L.S.(1992),Chenille Microfiber Mop Head. Biotechnology letters 14,6, 531-534[Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring]

Heidemann, E.(1997),HDPE Auto-Deflashing Plastic Bottle Blowing Machine.Leder 48,(5),99-104[Co2 Cylinders Cycling]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Aluminum Carpet Topping Stage Platform]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Economy Combo Heat Press for Starter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Perforated PVDF Grille Screen Panels Laser Cutting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack]

316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Cost Prefab Steel Poultry Chicken House on Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042]

316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[304 Precision Professional Dimpled Stainless Steel Plate]

316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Blue Color Fish Paper/6520/6521 Flexible Composites, Part I.Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Compression Mens Customized Gym Shorts Sportswear]

High Quality Factory 8ml UV Spider Gel Nail Care, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers.Plastic Grip Wood Handle Shovel, 175-184[Animal Jigsaw Floor Puzzles Education Learning Toys for Kid]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng]

Whitaker,J.R.(1963),2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve. Analytical Chemistry35(12), 1950-1953[Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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