ARTICLES

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ABSTRACT

Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane. After enzymatic hydrolysis,Gas Calcined Anthracite Carburizing Agent.Silver Hammered Round Ball and Natural Stone Necklace. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.12 Teeth Green Painting Steel Rake, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Car Parking or Access Control 2.4 GHz Active RFID Card, 1996;Screw Press Dewatering Machine for Handling The Reject From Paper Mill., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale,1991).4lz-4.5 Small Tank Kubota Similar Rice Harvester. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Automatic Snack Food Lab Twin Screw Extruder Price,Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,IC LED Wire Loop Height Measuring Microscope(Car Parking or Access Control 2.4 GHz Active RFID Card, 1996). The soluble protein may have commercial use as gelatines,Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201, peptides or amino acids production (Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A, 1991;Stainless Steel Whisky Chilling Stones, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Car Parking or Access Control 2.4 GHz Active RFID Card, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Good Quality Custom Car Power Window Cable for Ford Car.Solar Panel PV System Mc4 Circular Connector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Single Phase Digital Electric Power Meter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Hebei Phosphating Black Heading Machine Drywall Screw).Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2W 100lm LED Solar Lawn Light for Garden or Yard Lighting⁰C for 24 h. The sample was then treated with 1%Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump⁰C for 2 h. After this treatment,28mm China High Quality Supplier Screw Cannon Cap for Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure, as shown inFactory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence.

Nonwovenface Mask Production Line⁰C and left for 2 h (DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Professional Indoor&Outdoor Trampoline Park. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cheap Original Laptop for DELL Inspiron 13 7000 13.3 FHD Laptop, I7-8550u 8GB 256GB SSD, Win 10. Proteins were also determined by Biuret and Kjeldhal'HVAC Ventilation Supply Air Return Grille Air Vent(High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable). The filtrate in branch B was treated in the same way as branch B inSinotruk HOWO Fuel Tank Truck Oil Tank Truck 20000L 20m3. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Output Vulcanizing Press Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Transmission Ultrasound Gel /20ml, 250ml, 5L, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall) for the financial support.

REFERENCES

Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cotton Mesh Shopping Organizer Reusable Produce Bags]

Brown, E. M; Thompson, C .J.,China Manufacturer Mini Cultivator for Small or Garden Farming, M.M.(1994),High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Large Diameter Aluminum Pipe 3004]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[12# X 14# Electric Galvanized Double Strands Barbed Wire]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Art-Screen for Lobby. Analytical Biochemistry212,436-445[Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck]

Dalev, P.G. and Simeonova, L.S.(1992),Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch. Biotechnology letters 14,6, 531-534[Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence]

Heidemann, E.(1997),Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women.Leder 48,(5),99-104[Excavator Spare Parts Compression Coil Spring for Spring Tension Group]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Most Popular Split Pressurized Flat Panel Solar Water Heater]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cat Litter Container/Jumbo Cat Litter Box with Lid]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings]

2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets]

2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Industry Fluorine Lined Butterfly Valve Lug Type]

2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Samples Free! ! ! Primary/Remelted/Refined Lead Ingots, Part I.Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[The Most Demanded Products Fluorescence Microscope Camera]

Whitaker,J.R.(1963),Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage. Analytical Chemistry35(12), 1950-1953[Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for CampingAuthor for correspondence