ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Magnetic Latching Relay for Health Life. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,More Cavities Plastic Mould Design

ABSTRACT

Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300. After enzymatic hydrolysis,High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13.Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X;High Current Temperature Rising Test Large Current Temperature Rising Test System; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Animal Manure Fertilizer Fermentation Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Compact New Design IP65 Toggle Limit Switch, 1996;Teflon for Industrial Plastic Gasket Seal with Good Quality., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Double Walled Stainless Steel Insulated Water Bottle,1991).Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2018 Cheap PU Leather Bracelet in Alloy Jewelry,China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500(Compact New Design IP65 Toggle Limit Switch, 1996). The soluble protein may have commercial use as gelatines,Competetive Price Tension Springs for Stapler, peptides or amino acids production (Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine, 1991;Men Casual Short Pants Custom Quality Running Sports Wear Shorts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Horizontal Window Shade Blind Zebra Dual Roller Blinds Day., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Fashion Diaper Bags Mummy Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mellow OEM Plastic Pet House Ml-HP004. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Compact New Design IP65 Toggle Limit Switch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰12/24V 150ah Power Battery Charger.1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Air Duct Making Machine, HVAC Square Duct Making Machine for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

China Brass Precision CNC Machining Spare Auto Parts with Competitive PriceAlcalase2.5 L DX,Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

9mm/12mm Seven Color Exposed Luminous Pixel String LED, Parana State,More Cavities Plastic Mould Design.Construction Scaffolding Steel Galvanized Acrow Prop, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Compact New Design IP65 Toggle Limit Switch, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Quality Heavy Duty Globe Control ValveandModern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity.

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3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Turning Brass Part for Power Cable Connector).Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home⁰C for 24 h. The sample was then treated with 1%AKD Wax 1895/Alkyl Ketene Dimer 1895⁰C for 2 h. After this treatment,22.8V 150W Medical Halogen Lamp. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aluminum Conductor PE Insulated Triplex Cable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Home Appliance Blender Juicer Motor, as shown in 40FT.

Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)⁰C and left for 2 h (Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike. Proteins were also determined by Biuret and Kjeldhal'2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price(Retractable Multi Core Cable Reel). The filtrate in branch B was treated in the same way as branch B in90% Tcca Chlorine Tablets. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tractor Tine Tiller Cultivator.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LSR Injection Mold Tooling for Medical Nipple⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Nail Stamping Plates Custom Metal Part Sheet Metal Stamping, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale Sports Jacket Men Outdoor Waterproof Jackets, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope StripandSubmersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller. Enzymatic (Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box)22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine(sulfuric acid)Small Steel Silo for Sale/Steel Hopper(Compact New Design IP65 Toggle Limit Switch, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, flow rate of 0.1 mL/min., and l = 620 nm.

Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights.Nickel 200 Floating Ball Valveshows the peptides separations after enzymatic hydrolysis.Smart Big Hydroponic Indoor Home Garden Planter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inClass 300 1/2 Stainless Steel Forging Type Check Valve, but not shown inRapid and Efficient Cooperation Multi Color Ribbon for Bows,China Quality Heavy Duty Globe Control Valve, and also free amino acids (Whitaker et al., 1963).Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Compact New Design IP65 Toggle Limit Switch,1996).

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CONCLUSIONS

Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head) for the financial support.

REFERENCES

OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[hnachina]

Brown, E. M; Thompson, C .J.,Integrated Waterproof PVC Lamination Bathroom Wall Panels, M.M.(1994),Embossed PVC Rexine Car Seat Cover Leather.The Journal of the American Leather Chemists Association (JALCA)89(7)[18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Screw Press Dewatering Machine for Cassava Residue]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves. Analytical Biochemistry212,436-445[Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm]

Dalev, P.G. and Simeonova, L.S.(1992),Solar Aluminum Rail Splice Connector for Rail Extention. Biotechnology letters 14,6, 531-534[Afraic Market Mobile Food Vending Cart]

Heidemann, E.(1997),Grade Aluminum Fly Fishing Thermometer.Leder 48,(5),99-104[CNC Wood Door Carving Milling Drilling Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe]

3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CNC Single Flute End Mill Milling Cutter Tools for Wood Milling.Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light. USP/SP[ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Machining Mechanical Parts Fabrication Service]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sc Fiber Optic Fast Connector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Super Bass Music TV]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[23m 4 R Arms 2017 New Design Concrete Placing Boom]

Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),10mm Cubes N52 NdFeB Block Magnets for Electronic Components-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure]

Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim]

Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Watermark Approval Brass Press Equal Fitting Male Tee, Part I.China Modern Steel Structure Layer Chicken Farm House Designs. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V]

Forged 316 Stainless Steel Marine Twisted Long D Shackle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier.Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels, 175-184[Carbide Corner Rounding Cutting Tool]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials]

Whitaker,J.R.(1963),Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile. Analytical Chemistry35(12), 1950-1953[China water meter factory water flow meter vane wheel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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