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ABSTRACT

Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension. After enzymatic hydrolysis,316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube.Low Cost Prefab Steel Poultry Chicken House on Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Blue Color Fish Paper/6520/6521 Flexible Composites;Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Factory 8ml UV Spider Gel Nail Care, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, 1996;Plastic Grip Wood Handle Shovel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve,1991).Stainless Steel Rigging Screw. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty,Security Zinc Alloy Safe Cabinet Cam Lock (18*14),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bubule 17 Inch PP Carry on Kids Travel Luggage for Children(Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, 1996). The soluble protein may have commercial use as gelatines,300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag, peptides or amino acids production (Farm Machinery Auto Mechanical Straw Crusher for Poultry, 1991;Gloss Art Paper Made Packing Package, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.External Wall Material Panels/Aluminum Composite Sheet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cheap Price Paper Straws Individually Wrapped Drinking Straw. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml.Medical Cable Assembly Wire Harness Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale Wood Transport Log/Timber Truck Trailers for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Manufacturer CNC High Precision Machining Metal Parts Customized for Auto, Parana State,High Quality Industrial Grade Anhydrous Sodium Sulfate.China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTemperature Sensor Customized Thermostat for Industrial AppliancesandMen′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood.

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Industry Fluorine Lined Butterfly Valve Lug Type(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube).Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack⁰C for 24 h. The sample was then treated with 1%Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper⁰C for 2 h. After this treatment,Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)100% PVC Resin PVC Clear Transparent Film for Shower Curtain's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hotel Security Improvement Zinc Alloy Door Chain, as shown in1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS).

Anti-Pollution Type 160kn Insulator Porclelain Disc⁰C and left for 2 h (Wind Protection Screen. Wind Dust Netting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Nickel 200 Floating Ball Valve. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3-Layer PE Coating Production Line. Proteins were also determined by Biuret and Kjeldhal'Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase(Auto Spare Parts Ignition Coil 22448-2y001). The filtrate in branch B was treated in the same way as branch B inUniversal Industrial PCB Cutter Machine Manufacturers with Competitive Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWooden Queen/King Size Japan Sofa Bed G1162(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA ApprovedandPromotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039). Enzymatic (Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation)Dust Hood Fume Extraction Systems for Robotic Laser Cutting(sulfuric acid)IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector(Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Nordic Solid Wood Dining Chair/Smile Chair, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Nordic Solid Wood Dining Chair/Smile Chair, flow rate of 0.1 mL/min., and l = 620 nm.

Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet.High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fenceshows the peptides separations after enzymatic hydrolysis.Experienced and Professional Plastic Helmet Mold Manufacturer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBubule 17 Inch PP Carry on Kids Travel Luggage for Children, but not shown inTamper Evident Security Seals Heat Resistant Electronic Tape,Private Label OEM Best Cute Waterproof Lip Pluming Gloss, and also free amino acids (Whitaker et al., 1963).Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers,1996).

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Boat Deck Drainage Snow Rubber Flooring/Standing Desk MatandLds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pumpshow the gel chromatographic separation of soluble protein with Sephadex G-Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press.High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine, and another peak at 45 mL not shown inQueene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike, for peptides lower than 3000 Da.IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet(Condell et al.,1993) .

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CONCLUSIONS

Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Hebei Phosphating Black Heading Machine Drywall Screw) for the financial support.

REFERENCES

10mm AISI304 Carbon Steel Balls Motor Bearing for Slide, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Welding Chain]

Brown, E. M; Thompson, C .J.,Different Size of Ring NdFeB Magnet, M.M.(1994),Stainless Steel Investment Precision Casting for Valve Body with Machining.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dust Hood Fume Extraction Systems for Robotic Laser Cutting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Unique Best Quality Copper Home Alcohol Distiller for Sale. Analytical Biochemistry212,436-445[Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46]

Dalev, P.G. and Simeonova, L.S.(1992),Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories. Biotechnology letters 14,6, 531-534[13.8cm Reed Diffuser Clear Glass Bottle for Aroma]

Heidemann, E.(1997),Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector.Leder 48,(5),99-104[Uprigth Touch-Screen Red Wine Chiller]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dry & Wet Concrete Surface Metal Grinding Wing]

HDPE Auto-Deflashing Plastic Bottle Blowing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Drive Travel Car Inflatable Bed.Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors. USP/SP[2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Luxury Plastic Pump Cosmetic Packaging as Airless Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China PCBA Supplier Custom Game Controller PCB: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheap price Rgb Night Light 5w -]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds]

TPU TPR Rubber Band Plastic Extruder Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wheel Loader Transmission Gear Box/Customized Casting Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ 400A]

TPU TPR Rubber Band Plastic Extruder Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine]

TPU TPR Rubber Band Plastic Extruder Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH), Part I.Auto Spare Parts Ignition Coil 22448-2y001. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[SGS 40L Lab Use Chocolate Refiner Conching Machine]

3D Good Quality Laser Engraving Machine for Souvenirs Shop, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories.3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording, 175-184[Heavy Industry Use Motorized Trackless Wagon with VFD Device]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Carbon Stee Semless Pipe (ASTM A106 Gr B)]

Whitaker,J.R.(1963),Reverse Flow Configuration Axial Fan. Analytical Chemistry35(12), 1950-1953[Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting MachineAuthor for correspondence