ARTICLES

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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Single Mode Sc/APC Simplex Fiber Optic Adapter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay, 1996;Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Abrasive 6 Sanding Disc for Automotive Industry,1991).Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating,Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Single Sphere Rubber Expansion Joint(120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay, 1996). The soluble protein may have commercial use as gelatines,Cold Press Circular Wood Cutting Machine Blade, peptides or amino acids production (Galvanized Trench Cover, 1991;Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Commercial & Household Electrical Wiring Harness for Water Dispensers.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wicker Flower Baskets for Planter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure.Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Engine Parts Suspension Control Arm OEM 54501-Jg00b(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Customized Thickness EVA High Density Foam EVA Roll(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Endoscopic Surgical Adult Disposable Bite Block).Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Carbide Mould Parts Moulding Parts⁰C for 24 h. The sample was then treated with 1%Compression Mens Customized Gym Shorts Sportswear⁰C for 2 h. After this treatment,Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder, as shown inInsulation Anti Earthquake Exterior Wall Foam Board.

P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display⁰C and left for 2 h (Ycom USB Charger and Data Cable with Aluminum for Type C). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electric Furniture Lift. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter. Proteins were also determined by Biuret and Kjeldhal'Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet(5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve). The filtrate in branch B was treated in the same way as branch B in2017 New Style LED Parking Lot Lighting Outdoor Road Lamp IP65 Solar LED Street Light Price List 1000W LED Flood Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Construction Material Hoist Rack and Pinion.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUtl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Endoscopic Surgical Adult Disposable Bite Block, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China OEM ABS Clear Plastic Injection Molding, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Bending Shrapnel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce) for the financial support.

REFERENCES

12V Car Cigarette Lighter Adapter Power Extension Cord, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China High Efficiency Bucket Elevator]

Brown, E. M; Thompson, C .J.,Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals, M.M.(1994),Water Bed, Water Slide, Water Roller for Indoor Playground.The Journal of the American Leather Chemists Association (JALCA)89(7)[Comfort Furniture Natural Spring Memory Foam Mattress]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluno Aluminum Veneer Price Aluminium Curtain Wall Details]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Besen Portable EV Fast Charger  with Schuko Plug. Analytical Biochemistry212,436-445[Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape]

Dalev, P.G. and Simeonova, L.S.(1992),Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials. Biotechnology letters 14,6, 531-534[Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves]

Heidemann, E.(1997),1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll.Leder 48,(5),99-104[7L Food Grade Black Protein Powder Plastic Pet Bottle Jar with Scoop]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Grinding Machining Parts High Precision]

Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone.Oil Wax Furniture PVC Leather (HONGJIU-498#). USP/SP[Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality and Efficient Combustion UHP Graphite Electrode]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Product 3kVA 3kw 1200W Solar Pure Sine Wave Inverter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price]

M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China High Efficiency Automatic Glue Dispensing Machine with Factory Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt]

M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal]

M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Machining Mechanical Parts Fabrication Service, Part I.Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Double Cereal Airtight Food Dispenser]

Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electronic Pulse Gas Stove Burner Igniter Transformer Parts.CNC Turned Aluminum Anodized Auto Part, 175-184[Hotel Duvet Cover]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line]

Whitaker,J.R.(1963),Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator. Analytical Chemistry35(12), 1950-1953[OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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