ARTICLES

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ABSTRACT

Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration. After enzymatic hydrolysis,PA Flexible Plastic Corrugated Loom Tubing.Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3-Way T Type Elbow Quick Connect Pneumatic Fitting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Superior Quality Soya Protein Food Production Machine Line, 1996;Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Army Style 100% Polyester Fiber Mens Polo Shirt,1991).E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Twisted Annealed Wire for Binding,Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Fashion Jewellery Lady Eardrop Earrings for Women(China Superior Quality Soya Protein Food Production Machine Line, 1996). The soluble protein may have commercial use as gelatines,High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640, peptides or amino acids production (Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings, 1991;Full Automatic AAC Concrete Lightweight Block Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel CF8/CF8m Street Elbow Pipe Fittings., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Superior Quality Soya Protein Food Production Machine Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts.Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Low Volume Production Aluminum CNC Machining Auto Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale).Women′s Seamless Strapless Bra(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet⁰C for 24 h. The sample was then treated with 1%300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm⁰C for 2 h. After this treatment,Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw, as shown inRide on Toys Riding Animals in The Mall.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wind Protection Screen. Wind Dust Netting, Wind Dust Net. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av24K Gold Rolling Paper Shine Papers Cigarette Paper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ride on Toys Riding Animals in The Mall, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Finely Processed Pneumatic Operated Globe Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Workbench Anti-Static Table ESD Natural Rubber Table Mat. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Carbon Steel Round/Square Pipe Tube Production Line) for the financial support.

REFERENCES

316L 10 Micron Sintered Stainless Steel Filter Disc, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Immersion Gold Asic Miner Pcb Manufacturer]

Brown, E. M; Thompson, C .J.,Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket, M.M.(1994),Abstract Light and Shadow Metal Oil Painting for Deco.The Journal of the American Leather Chemists Association (JALCA)89(7)[355nm UV Laser Marking Equipment for Crystal Materials]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[EAS Anti-Theft Optical Security Tag for Glasses Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003). Analytical Biochemistry212,436-445[Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware]

Dalev, P.G. and Simeonova, L.S.(1992),Cheapest Container Living House/Office/Kiosk. Biotechnology letters 14,6, 531-534[Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain]

Heidemann, E.(1997),Spin Centrifugal Demister Separator or Column Internal.Leder 48,(5),99-104[Car Cup Cooler And Warmer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Women′s Seamless Strapless Bra]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[J55 Short Round Thread Seamless Casing Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Adjustable Grooming Table]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cattle Panel Hot Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Low Cost Prefab Steel Poultry Chicken House on Sale]

360 Degree Reciprocating Car Wash Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Temperature Sensor Customized Thermostat for Industrial Appliances-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade]

360 Degree Reciprocating Car Wash Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Elbow SS304 SS316L]

360 Degree Reciprocating Car Wash Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Die Aluminum Castings, Part I.High Quality Meat Pie Making Machine with Different Mould Shape. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cracking Acidification Clay Stabilizer Pale Yellow Liquid]

Gear Operated Flanged High Performance Butterfly Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck.Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3, 175-184[Rubber Foam Adhesive Insulation Sheet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]

Whitaker,J.R.(1963),Wholesale Universal Garden Smart Flower Pot. Analytical Chemistry35(12), 1950-1953[Making Machine for EVA Soft Hose]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008Author for correspondence