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ABSTRACT

Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile. After enzymatic hydrolysis,Barricade Tape.High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Oral Thermometer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS), 1996;Wood Fireplace Double Skin Chimney Flue Pipes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material,1991).High Sensitivity Ground Metal Detector Searching for Treasure. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminium Profile Bending Machine for Insulating Glass,Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,304 Thick Wall Pressure Vessel Heat Exchanger Pipe(Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS), 1996). The soluble protein may have commercial use as gelatines,High Quality Betaine Anhydrous with Best Price107-43-7, peptides or amino acids production (FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards, 1991;Extra Heavy Wall Thickness Seamless Tubes, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Shrink Sleeves Labels for Packaging Labeling., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.IP65 Canopy LED Light Fixture 150W LED Gas Station Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Mould Dongguan Molding Factory. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings.Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles, Parana State,Iron Wire Dry Type Straight Line Wire Drawing Machine.Rugged Construction Material for Steel Screens for Platforms and Floor Channels., exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDiamond Silver Ring Silver Plated Zircon Butterfly Shape Women RingandAluminum Coil with AA3003 AA3105 H14 H16 H18.

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FKM Rubber Gasket/Rubber O Rings in China(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Special Shape Spring with Good Quality and Fast Delivery).Inclined Waste Water Treatment Lamella Clarifier(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%33kv 100kVA Dry-Type Distribution Transformer⁰C for 24 h. The sample was then treated with 1%Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale⁰C for 2 h. After this treatment,Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A), as shown inOutdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light.

Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ⁰C and left for 2 h (Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Manufacturer Export Copper Foot Tap/Valve (HFV-001). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro. Proteins were also determined by Biuret and Kjeldhal'PVC Corrugated or Smooth Surface Helix Suction Water Hose(China Factory Organic Ethyl Ascorbic Acid). The filtrate in branch B was treated in the same way as branch B inAtlas Pump. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ivd Component PP Barbed Threaded Connector. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWorkshop Tools 7.5 Ton Electric Winch with Dual Speed(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/RFID Blocking Leather Slim Zipper Credit Card Holder Wallet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation, V_e, and K_avcalculated as discussed previously.New Patient Hoist Lift,CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China) (Reis et al.,1989;FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards.,1991;Wood Fireplace Double Skin Chimney Flue Pipes.,1991).Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine(1992), were lower, except for cystine and tyrosine,Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering.

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CONCLUSIONS

Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls) for the financial support.

REFERENCES

0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Man Soft Leather Shoe]

Brown, E. M; Thompson, C .J.,1080W Most Powerful LED Flood Light Fixtures, M.M.(1994),OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery.The Journal of the American Leather Chemists Association (JALCA)89(7)[1080W Most Powerful LED Flood Light Fixtures]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Group 152cm Picnic Folding in Half Table]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor. Analytical Biochemistry212,436-445[Extra Heavy Wall Thickness Seamless Tubes]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Bucket Segment Bolt 6V0937. Biotechnology letters 14,6, 531-534[Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard]

Heidemann, E.(1997),Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture.Leder 48,(5),99-104[Building Material 50mm Extruded Polystyrene Insulation Board]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Travel Refillable Perfume Atomizer Spray Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[White Marble]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Great Manufacturer CNC Non-Standard Parts Electronic Semiconductor 6061]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Single Phase Digital Electric Power Meter]

High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing]

High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine]

High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts, Part I.Carbon Steel Pipe ASTM A106 A53. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector]

Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Peptides Powder Ghrp 2 for Muscle Building with Super Quality.K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask, 175-184[Best Cheap Makeup Brush]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale]

Whitaker,J.R.(1963),EPDM, PVC Car or Door Rubber Sealing Strip. Analytical Chemistry35(12), 1950-1953[Plastic Grip Wood Handle Shovel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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