ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Barricade Tape. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip

ABSTRACT

Fully Automatic Industrial Microwave Dehydrator. After enzymatic hydrolysis,Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L).Oral Thermometer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Sensitivity Ground Metal Detector Searching for Treasure, 1996;Aluminium Profile Bending Machine for Insulating Glass., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws,1991).304 Thick Wall Pressure Vessel Heat Exchanger Pipe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Betaine Anhydrous with Best Price107-43-7,FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Extra Heavy Wall Thickness Seamless Tubes(High Sensitivity Ground Metal Detector Searching for Treasure, 1996). The soluble protein may have commercial use as gelatines,Shrink Sleeves Labels for Packaging Labeling, peptides or amino acids production (IP65 Canopy LED Light Fixture 150W LED Gas Station Light, 1991;Plastic Mould Dongguan Molding Factory, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Sensitivity Ground Metal Detector Searching for Treasure, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles.Good Quality Banded Type Tee-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (360 Degree Reciprocating Car Wash Machine).Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring⁰C for 24 h. The sample was then treated with 1%Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)⁰C for 2 h. After this treatment,Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc . The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Manufacturer Export Copper Foot Tap/Valve (HFV-001)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro, as shown inHigh Quality Endoscopic Surgical Adult Disposable Bite Block.

PVC Corrugated or Smooth Surface Helix Suction Water Hose⁰C and left for 2 h (Insulation Anti Earthquake Exterior Wall Foam Board). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Ivd Component PP Barbed Threaded Connector. Proteins were also determined by Biuret and Kjeldhal'Workshop Tools 7.5 Ton Electric Winch with Dual Speed(Ycom USB Charger and Data Cable with Aluminum for Type C). The filtrate in branch B was treated in the same way as branch B inSnmp. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2017 New Design Vinyl 3D Stone Wallpaper Factory in China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Small Paper Gift Box with Magnet Closure for E-Cigarette, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Agricultural Mini Tiller Farm Wheel 62cc Cultivator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automotive Sheet Metal Stamping Car Auto Parts.Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)shows the peptides separations after enzymatic hydrolysis.Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSingle Sphere Rubber Expansion Joint, but not shown inWhite Pattern Acrylic Sheet for Decoration Produced by Senpu,Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable, and also free amino acids (Whitaker et al., 1963).2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Sensitivity Ground Metal Detector Searching for Treasure,1996).

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CONCLUSIONS

OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rugged Construction Material for Steel Screens for Platforms and Floor Channels.) for the financial support.

REFERENCES

Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.hdfmills.com]

Brown, E. M; Thompson, C .J.,High Quality Bucket Segment Bolt 6V0937, M.M.(1994),Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture.The Journal of the American Leather Chemists Association (JALCA)89(7)[Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Comfort Furniture Natural Spring Memory Foam Mattress]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor Server Rack Network Data Cabinet. Analytical Biochemistry212,436-445[Aluno Aluminum Veneer Price Aluminium Curtain Wall Details]

Dalev, P.G. and Simeonova, L.S.(1992),Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera. Biotechnology letters 14,6, 531-534[Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape]

Heidemann, E.(1997),Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House.Leder 48,(5),99-104[Antique Glass Jar]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Investment Casting Lost Wax Casting Precision Casting Stainless Steel Valve Parts Adapter Flanges]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality and Efficient Combustion UHP Graphite Electrode]

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Carbon Steel Pipe ASTM A106 A53,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt]

Carbon Steel Pipe ASTM A106 A53, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Peptides Powder Ghrp 2 for Muscle Building with Super Quality, Part I.K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal]

EPDM, PVC Car or Door Rubber Sealing Strip, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Black Color Tube Eyelet Lift Gas Spring.Safety Edge Sensor for Agv Automated Guided Vehicle, 175-184[Factory Supply PVC Coated Double Wires Welded Mesh Fence]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Double Cereal Airtight Food Dispenser]

Whitaker,J.R.(1963),Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets. Analytical Chemistry35(12), 1950-1953[High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM Light-Weight Aluminium Die Casting Casing Parts for Auto PartsAuthor for correspondence