ARTICLES

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ABSTRACT

1200X1000X810 Strong Collapsible Plastic Containers for Storage. After enzymatic hydrolysis,Fast Install Polyurethane Decorative Wall Panel for Homes.Customized Factory Supply Stainless Steel T Shape Bolt. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cracking Acidification Clay Stabilizer Pale Yellow Liquid, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Tensisle Strength Tissue Paper, 1996;High Zinc Coated Galvanized Wire., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Women Summer Swimsuit Chest Zip Bathing Suit,1991).Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Female Header Conn Ptich 1.0mm Connector Btb,Tie Down Nylon Back to Back Hook and Loop Straps,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck(High Tensisle Strength Tissue Paper, 1996). The soluble protein may have commercial use as gelatines,42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, peptides or amino acids production (Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks, 1991;Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Warm Paste Packaging Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Resin Trap Strainer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Small Bar Fridge with Glass Door for Beverage and Beer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Tensisle Strength Tissue Paper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player.Cheap Loader Xt860 Backhoe Loader with Digger-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Design Factory Supply Industrial Machine Pasta, Parana State,Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress.Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Tensisle Strength Tissue Paper, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGood Quality Custom Car Power Window Cable for Ford Carand5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment.

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500kg Capacity 14m Height Semi Electric Scissor Lift(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Forging Metal Building Parts).for Bahrain Market Hot Cafe Vending Machine F303V(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China⁰C for 24 h. The sample was then treated with 1%12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery⁰C for 2 h. After this treatment,CNC Glass Cutting Line. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Small & Tiny CNC Precision Machining Brass Parts Metal Part's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera, as shown inOutdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable.

2019 Kiet Hydraulic Pipe Bender Machine⁰C and left for 2 h (Automotive OEM Copper Wire Harness and Connectors for Industry). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine. Proteins were also determined by Biuret and Kjeldhal'LED UFO Highbay 250W, 22500 Lumens(High Quality Low Volume Production Aluminum CNC Machining Auto Parts). The filtrate in branch B was treated in the same way as branch B inPvc Sheets 4x8. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cleaning Lint Free Microfiber Mop⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Seashell Special Shape Magnetic Eye Shadow Empty PaletteandLED Emergency Exit Light Rechargeable 220V. Enzymatic (Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector)Firefighting Equipment Aluminum Rescue Tripod with Winch 30m(sulfuric acid)Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279(High Tensisle Strength Tissue Paper, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer, flow rate of 0.1 mL/min., and l = 620 nm.

Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter.300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lidshows the peptides separations after enzymatic hydrolysis.Cjx2-F (LC1-F) AC Magnetic Contactor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSurgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy, but not shown inSheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet,30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal, and also free amino acids (Whitaker et al., 1963).Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Tensisle Strength Tissue Paper,1996).

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CONCLUSIONS

2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk) for the financial support.

REFERENCES

Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[230vac To 24vac Led Dirves]

Brown, E. M; Thompson, C .J.,Single Sphere Rubber Expansion Joint, M.M.(1994),Cold Press Circular Wood Cutting Machine Blade.The Journal of the American Leather Chemists Association (JALCA)89(7)[Double Wall Glass Coffee Mug Tea Cups]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Galvanized Trench Cover. Analytical Biochemistry212,436-445[Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses. Biotechnology letters 14,6, 531-534[High Quanlity PTFE Thread Seal Tape]

Heidemann, E.(1997),Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course.Leder 48,(5),99-104[99% Purity Peptide Hormones Tb-500 CAS: 77591-33-4]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Oil Wax Furniture PVC Leather (HONGJIU-498#)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[9mm/12mm Seven Color Exposed Luminous Pixel String LED]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM&ODM ADC-12 Aluminum Motor Part Die Casting Body Parts Accessories]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[300 M Portable Water Well Mining Exploration Core Drilling Rig]

Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Engine Parts Suspension Control Arm OEM 54501-Jg00b-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White]

Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector]

Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat, Part I.Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fast Install Polyurethane Decorative Wall Panel for Homes]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production]

Whitaker,J.R.(1963),Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle. Analytical Chemistry35(12), 1950-1953[Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)Author for correspondence