ARTICLES

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ABSTRACT

Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11. After enzymatic hydrolysis,Single Mode Sc/APC Simplex Fiber Optic Adapter.120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine;Abrasive 6 Sanding Disc for Automotive Industry; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating, 1996;Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Single Sphere Rubber Expansion Joint,1991).Cold Press Circular Wood Cutting Machine Blade. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Galvanized Trench Cover,Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course(2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating, 1996). The soluble protein may have commercial use as gelatines,Commercial & Household Electrical Wiring Harness for Water Dispensers, peptides or amino acids production (Wicker Flower Baskets for Planter, 1991;5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Engine Parts Suspension Control Arm OEM 54501-Jg00b.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker.Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Carbide Mould Parts Moulding Parts, Parana State,Aluminum/Aluminium Extrusion Profile Housing for LED Light.Compression Mens Customized Gym Shorts Sportswear, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the AgrochemicalsandSmall Brown Glass Bottles.

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Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB).99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder⁰C for 24 h. The sample was then treated with 1%P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display⁰C for 2 h. After this treatment,Electric Furniture Lift. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet, as shown inBiostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer.

Construction Material Hoist Rack and Pinion⁰C and left for 2 h (Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks. Proteins were also determined by Biuret and Kjeldhal'Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal(2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve). The filtrate in branch B was treated in the same way as branch B inChillers/Freezer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Endoscopic Surgical Adult Disposable Bite Block. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina OEM ABS Clear Plastic Injection Molding(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Black Powder Coated Steel Sheet Metal Housing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sell 100% Seal Hexagon Glass Jam Jar, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Coill Nail Making Machine/Coil Nail Welding MachineandMetal Parts Stamping, Stamped Brass Shrapnel,   Brass Connect Stamping, Electronic Connection Piece Metal Stamped Part, Copper, Brass Connect Stamping. Enzymatic (High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair)Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape(sulfuric acid)Heavy Caliber Copper Rectangular Steel Tube(2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2mm White Coated Paper Cake Boards Jordan, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2mm White Coated Paper Cake Boards Jordan, flow rate of 0.1 mL/min., and l = 620 nm.

Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box.10mm Tempered Bathroom Glass Safety Glass for Buildingshows the peptides separations after enzymatic hydrolysis.Luxious Hotel Passenger Elevator, Titanium Elevator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAmerican NEMA Straight Blade Plug 30A 125V/250V Power Plug, but not shown inOpen Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector,Yuchai 400kw Diesel Generator Set, and also free amino acids (Whitaker et al., 1963).Black Plastic GRP FRP PE Pipe Fitting for Water Supply. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating,1996).

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P4.75 Indoor Single Dual Color Advertising LED Display BillboardandChina Cheap Newest 70*77cm XPE Foam 3D Wallpapershow the gel chromatographic separation of soluble protein with Sephadex G-2194230012 for Mercedes Benz Auto Spare Parts Brake Disc.Precision Casting Factory Foundry OEM Ductile Iron Sand Casting, and another peak at 45 mL not shown in500kg Capacity 14m Height Semi Electric Scissor Lift, for peptides lower than 3000 Da.Hot Sale Electrical Motors Small for Home Appliance(Condell et al.,1993) .

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PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line, V_e, and K_avcalculated as discussed previously.Bending Shrapnel,12V Car Cigarette Lighter Adapter Power Extension Cord. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443)) (Reis et al.,1989;Wicker Flower Baskets for Planter.,1991;Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm.,1991).Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals(1992), were lower, except for cystine and tyrosine,Water Bed, Water Slide, Water Roller for Indoor Playground.

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CONCLUSIONS

Besen Portable EV Fast Charger  with Schuko Plug. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Thickness EVA High Density Foam EVA Roll) for the financial support.

REFERENCES

Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Block Making Machine]

Brown, E. M; Thompson, C .J.,1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll, M.M.(1994),UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Anti-Corrosion Fiberglass Sheet and Roll]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[E6000 Handheld Three Phase Power Quality and Energy Analyzer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Oil Wax Furniture PVC Leather (HONGJIU-498#). Analytical Biochemistry212,436-445[Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools]

Dalev, P.G. and Simeonova, L.S.(1992),Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine. Biotechnology letters 14,6, 531-534[Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision]

Heidemann, E.(1997),M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector.Leder 48,(5),99-104[29 Mountainbike]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2018 New Design Red-CNC-006 CNC Machining Aluminum Precision]

Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China High Efficiency Automatic Glue Dispensing Machine with Factory Price.CNC Machining Mechanical Parts Fabrication Service. USP/SP[Unique Best Quality Copper Home Alcohol Distiller for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cheap Price Paper Straws Individually Wrapped Drinking Straw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Customized Mug Heat Press Machine Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Enclosed Fiber Laser Marking Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[5g OPP Silica Gel Desiccant]

Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electronic Pulse Gas Stove Burner Igniter Transformer Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ivd Component PP Barbed Threaded Connector]

Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine]

Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Turned Aluminum Anodized Auto Part, Part I.Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor Server Rack Network Data Cabinet]

Commercial Vegetable Mushroom Coconut Meat Drying Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass.Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, 175-184[Wholesale High Quality Custom Frosted Glass Candle Jar]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Custom Round Aluminum Tube 5154 5454 5652 5086]

Whitaker,J.R.(1963),Hydraulic Fold-Bend Machine Hydraulic Bending Machine. Analytical Chemistry35(12), 1950-1953[Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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