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ABSTRACT

Women Party Jewelry 45cm Sequins Choker. After enzymatic hydrolysis,New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity.High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory SMD3528 60LEDs/M Flexible LED Strip, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot-Press Small Continuous Diamond Saw Blade Cutting Disc, 1996;Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick,1991).Comfort Furniture Natural Spring Memory Foam Mattress. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume,4.2A Type a Type C Pd QC3.0 USB Charger,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ferrous Sulphate Monohydrate 30%Min Factory Wholesale(Hot-Press Small Continuous Diamond Saw Blade Cutting Disc, 1996). The soluble protein may have commercial use as gelatines,Small Portable Scooters Folding Electric Scooters for Adults, peptides or amino acids production (Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, 1991;DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Most Popular Split Pressurized Flat Panel Solar Water Heater.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot-Press Small Continuous Diamond Saw Blade Cutting Disc, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine.Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized UV Drying Heating Machine for Screen Printer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Geotextile for Roofs).China Products Suppliers Camlock Coupling Quick Couplings Female(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest⁰C for 24 h. The sample was then treated with 1%3.2m Vertical Wheelchair Elevator Platform for Sale⁰C for 2 h. After this treatment,LC Multistage Pump Mechanical Seal (KLLC). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wedding Tent Cooling System Outdoor Event Air-Con Units's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV, as shown inHigh Freqency PCB with Blind Via.

Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel⁰C and left for 2 h (China PCBA Supplier Custom Game Controller PCB). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Prime Gi Steel Strip Galvanized Metal Strip Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fireproof Aluminum Curtain Wall Panel. Proteins were also determined by Biuret and Kjeldhal'Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple(Luxious Hotel Passenger Elevator). The filtrate in branch B was treated in the same way as branch B inSt52 Bks Skiving or Burnishing Seamless Steel Pipe. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAdjustable Grooming Table(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PE Coated Paper for Salt Sugar Sachets Paper Food Grade⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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OEM High Pressure Machined Part Die Aluminum Casting, V_e, and K_avcalculated as discussed previously.AISI 430 Ba/2b Embossed Stainless Steel Sheet,High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Heavy Duty Steel Warehouse Storage Pallet Rack) (Reis et al.,1989;Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant.,1991;Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage.,1991).Drug Delivery ABS Hospital Medical Treatment Medicine Cart(1992), were lower, except for cystine and tyrosine,YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black.

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CONCLUSIONS

Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment) for the financial support.

REFERENCES

Colorful Electric English Funny Music Story Module Printing Book, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Blue Perfume Bottle]

Brown, E. M; Thompson, C .J.,2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine, M.M.(1994),IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650).The Journal of the American Leather Chemists Association (JALCA)89(7)[600mm Warehouse No Power Roof Fan]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[12 Teeth Green Painting Steel Rake]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tilt-and-Turn-with-Hidden-Hinge. Analytical Biochemistry212,436-445[HOWO 420HP Rhd Tractor Truck Head]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Soft PVC Granule Used for Refrigerator Door Gasket. Biotechnology letters 14,6, 531-534[Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet]

Heidemann, E.(1997),0.45um Dia 47mm PVDF Filter Membrane.Leder 48,(5),99-104[High Quality S Shape Scratcher Toys]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Style Custom Logo Makeup Multi Colored Eyeshadow Palette]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Slotted Round Head Aluminum Self Tapping Screw: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carbon Steel Hexagon Dome Cap Nut with Plain or Galvanized]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ce Approval Biscuit Trayless Packing Machine (MZ-250B)]

Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Classic Black Color Commercial Glass Door Multi Deck Refrigerator-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Commercial Vegetable Mushroom Coconut Meat Drying Machine]

Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Solid Steel Hotel Safe (G-42BD classics) for Hotel]

Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker, Part I.Hotbar Reflow Machine for FPC of Camera. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Carbon Steel Pipe ASTM A106 A53]

Full Viton Coated Half Shaft Wafer Butterfly Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Claus Process Catalyst 4-6 mm.4 Different Ball Silicone Ice Cube Maker, 175-184[America Best Selling Kids Electric Cars Children Ride on Car]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss]

Whitaker,J.R.(1963),High Quality Washable Durable Hotel Polyester Banquet Table Cloths. Analytical Chemistry35(12), 1950-1953[IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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