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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Yuchai 400kw Diesel Generator Set. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Black Plastic GRP FRP PE Pipe Fitting for Water Supply

ABSTRACT

2194230012 for Mercedes Benz Auto Spare Parts Brake Disc. After enzymatic hydrolysis,Precision Casting Factory Foundry OEM Ductile Iron Sand Casting.Hot Sale Electrical Motors Small for Home Appliance. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line;Bending Shrapnel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.12V Car Cigarette Lighter Adapter Power Extension Cord, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals, 1996;Water Bed, Water Slide, Water Roller for Indoor Playground., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Besen Portable EV Fast Charger  with Schuko Plug,1991).Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll,UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Oil Wax Furniture PVC Leather (HONGJIU-498#)(Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals, 1996). The soluble protein may have commercial use as gelatines,Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine, peptides or amino acids production (M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector, 1991;China High Efficiency Automatic Glue Dispensing Machine with Factory Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC Machining Mechanical Parts Fabrication Service., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electronic Pulse Gas Stove Burner Igniter Transformer Parts.CNC Turned Aluminum Anodized Auto Part-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Delphi Control Valve 28239295 (622B), Parana State,Black Plastic GRP FRP PE Pipe Fitting for Water Supply.OEM Precision Aluminum Alloy/Zinc Alloy Die Casting, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlastic Tk4100/T5577/S50/S70 Contactless RFID Card for IdentificationandStainless Steel Investment Precision Casting for Valve Body with Machining.

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Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Small Tin Box for Condoms).Bathroom Makeup LED Mirror Glass with Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence⁰C for 24 h. The sample was then treated with 1%Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D⁰C for 2 h. After this treatment,White and Green Board, School Writing Board, School Furniture (GT-76C). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Current Precise LED Digital Ammeter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Drinking Mineral Water Filling Line/Water Bottling Line, as shown inFan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201.

Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men⁰C and left for 2 h (Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PP Polypropylene Two Layer Film Blowing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Disposable Sugarcane Pulp Paper Bowl 500ml. Proteins were also determined by Biuret and Kjeldhal'Flat Roof Insulation Board Heat Insulation Board(5 Liter Packed Medical Ultrasound Gel with Ce and ISO). The filtrate in branch B was treated in the same way as branch B inwww.mybarberchairs.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Female Threaded Tee T Shape Pipe Fittings. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Outdoor Indoor Football Soccer Goal Post Net for Kids.DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinetshows the peptides separations after enzymatic hydrolysis.Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLivining Room Rugs Geometric Patterns Carpets 6′*9′, but not shown in4.2A Type a Type C Pd QC3.0 USB Charger,20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory, and also free amino acids (Whitaker et al., 1963).Hot Sale High Speed Mixer Blender for Extruder Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals,1996).

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CONCLUSIONS

White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Headband Big Headphone EVA Bag Tool Case Specialized Storage Box) for the financial support.

REFERENCES

Crusher for Animal Waste/ Chicken Manure, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Network Cable Copper Standard SFTP/FTP CAT6 LAN Cable Computer Monitor Security]

Brown, E. M; Thompson, C .J.,Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs, M.M.(1994),Threaded Locking Circular Connectors M16 680.The Journal of the American Leather Chemists Association (JALCA)89(7)[5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector. Analytical Biochemistry212,436-445[Claus Process Catalyst 4-6 mm]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card. Biotechnology letters 14,6, 531-534[316L 10 Micron Sintered Stainless Steel Filter Disc]

Heidemann, E.(1997),Disposable Sterile Safety Blood Lancet.Leder 48,(5),99-104[Brown Fused Alumina For Abrasive]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ceiling Mounted IV Stand Stainlesss Steel I V Pole]

Luxious Hotel Passenger Elevator, Titanium Elevator, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Plush Comfort Spring Mattress Cheap Price for Wholesale.3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss. USP/SP[Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Resin Trap Strainer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[insharevape.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Drug Delivery ABS Hospital Medical Treatment Medicine Cart]

Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment]

Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet]

Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain, Part I.Waterproof LED Flash Shoe Light Crystal Epoxy Resin. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Density Artificial Grass Football Playground Surfaces (STO)]

Custom Shopping Paper Bag for Clothing Package, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Qingdao Us Type Load Binder Lever Chain Tensioner.Stainless Steel Elbow SS304 SS316L, 175-184[High Quality Factory Cast Iron Butterfly Valves]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Manufacturer High Strength Carbon Steel Heavy Hex Nut]

Whitaker,J.R.(1963),5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD. Analytical Chemistry35(12), 1950-1953[Anti-Pollution Type 160kn Insulator Porclelain Disc]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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