ARTICLES

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ABSTRACT

Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts. After enzymatic hydrolysis,14 Gauge Twist Galvanized Black Annealed Steel Wire.2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fully Automatic Digital Pid Temperature Controller;High Quality Anti-Corrosion Fiberglass Sheet and Roll; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Full-Automatic Folding Napkin Paper Machine, 1996;Customized Frame Mount Highway Pegs Enclosure Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling,1991).10mm Tempered Bathroom Glass Safety Glass for Building. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet,Cattle Feed Factory Used Dust Collector and Filter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box(Full-Automatic Folding Napkin Paper Machine, 1996). The soluble protein may have commercial use as gelatines,Group 152cm Picnic Folding in Half Table, peptides or amino acids production (Tactile Custom Branded Membrane Switch Keyboard with Foil, 1991;304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Full-Automatic Folding Napkin Paper Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Australia Two Pins Power Cord with SAA Certification.87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Rectangular Lunch Tin Box with Handle, Parana State,380V 3.0HP Peristaltic Pump Swimming Pool Pump.Inflatable Cheering Stick for Promotional Gift, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Full-Automatic Folding Napkin Paper Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBgf-800 BOPP Dry Laminating MachineandSoft Rubber Hair Remover Pet Brush.

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Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (AKD Wax 1895/Alkyl Ketene Dimer 1895).Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2⁰C for 24 h. The sample was then treated with 1%Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket⁰C for 2 h. After this treatment,2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mini Outdoor Hydraulic Man Aluminum Lift's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cheap Price Roof Wall PU Sandwich Panel Press, as shown in13 Oz Flex Banner PVC Frontlit Banner Material.

Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use⁰C and left for 2 h (New Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit Hoodies). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.industrial aluminum foil roll 8011 8079 1235. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval. Proteins were also determined by Biuret and Kjeldhal'High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket(En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube). The filtrate in branch B was treated in the same way as branch B inForward Curved Pressure Blower. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Afraic Market Mobile Food Vending Cart. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Vibrating Condom Ring and Cock Ring Supplier, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230mlandFull Color Print Glossy Laminated PP Woven Shopping Gift Bag. Enzymatic (High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40)Quality Assurance Advanced Design Microfiber Filling Washable Pillow(sulfuric acid)Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price(Full-Automatic Folding Napkin Paper Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, flow rate of 0.1 mL/min., and l = 620 nm.

Wall Mounting Metal Control Box Control Desk.Outdoor 15kv Pole Mounted Automatic Circuit Reclosershows the peptides separations after enzymatic hydrolysis.Precision Tooling Grinding Machine Part(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChinese Top Manufacturer Aluminium LED Profile for LED Linear Light, but not shown inWood Fireplace Double Skin Chimney Flue Pipes,Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks, and also free amino acids (Whitaker et al., 1963).Kellogg′s Corn Flakes Cereals Snacks Food Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Full-Automatic Folding Napkin Paper Machine,1996).

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Respirator

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CONCLUSIONS

High Density Artificial Grass Football Playground Surfaces (STO). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml) for the financial support.

REFERENCES

High Quality Male Water Quick Release Coupling, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Epe Foam Fruit Net Plastic Machine]

Brown, E. M; Thompson, C .J.,Professional Manufacture Hydraulic Cylinder Tube, M.M.(1994),Chenille Microfiber Mop Head.The Journal of the American Leather Chemists Association (JALCA)89(7)[SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pipe Connection Types of Pressing Scaffolding Universal Clamp]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-HDPE Auto-Deflashing Plastic Bottle Blowing Machine. Analytical Biochemistry212,436-445[Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable. Biotechnology letters 14,6, 531-534[2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow]

Heidemann, E.(1997),High Quality Industrial Grade Anhydrous Sodium Sulfate.Leder 48,(5),99-104[Smart Car Spare Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle]

PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension.316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube. USP/SP[Australia Two Pins Power Cord with SAA Certification]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Low Cost Prefab Steel Poultry Chicken House on Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[UV Protective Highway Polycarbonate Solid Sound Barrier Sheet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Disposable Food Packaging Paper Soup Bowl with Lids]

Blue Color Fish Paper/6520/6521 Flexible Composites, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Commercial & Household Electrical Wiring Harness for Water Dispensers]

Blue Color Fish Paper/6520/6521 Flexible Composites,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet]

Blue Color Fish Paper/6520/6521 Flexible Composites, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Factory 8ml UV Spider Gel Nail Care, Part I.Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale]

Plastic Grip Wood Handle Shovel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve.Stainless Steel Rigging Screw, 175-184[cnbyapparel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ride on Toys Riding Animals in The Mall]

Whitaker,J.R.(1963),Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty. Analytical Chemistry35(12), 1950-1953[6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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