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ABSTRACT

Stainless Steel Handweel Three-Way Non-Retention Ball Valve. After enzymatic hydrolysis,New Design Factory Supply Industrial Machine Pasta.Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;500kg Capacity 14m Height Semi Electric Scissor Lift;for Bahrain Market Hot Cafe Vending Machine F303V; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, 1996;CNC Glass Cutting Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Small & Tiny CNC Precision Machining Brass Parts Metal Part,1991).Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 Kiet Hydraulic Pipe Bender Machine,PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine(12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, 1996). The soluble protein may have commercial use as gelatines,LED UFO Highbay 250W, 22500 Lumens, peptides or amino acids production (Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved, 1991;Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cleaning Lint Free Microfiber Mop. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm.Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LED Emergency Exit Light Rechargeable 220V(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Single Sphere Rubber Expansion Joint. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCold Press Circular Wood Cutting Machine Blade(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Galvanized Trench Cover(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Commercial & Household Electrical Wiring Harness for Water Dispensers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279) for the financial support.

REFERENCES

Electric Furniture Lift, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wrought Iron Floor Lamps]

Brown, E. M; Thompson, C .J.,Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter, M.M.(1994),Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Construction Material Hoist Rack and Pinion. Analytical Biochemistry212,436-445[High Output Vulcanizing Press Machine]

Dalev, P.G. and Simeonova, L.S.(1992),3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out. Biotechnology letters 14,6, 531-534[3-Layer PE Coating Production Line]

Heidemann, E.(1997),Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks.Leder 48,(5),99-104[Auto Parts Stainless Steel SS304 Ss 316 Hex Bolts and Nuts Zinc Plated Thread Bolt Hot DIP Galvanized 4.8 8.8 Hex Nut & Bolt (DIN933 AND DIN934)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Green PVC Coated Front Yard Double Wire Mesh Fence Panel]

Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal.2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses. USP/SP[ Win 10]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Super Bass Music TV]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Endoscopic Surgical Adult Disposable Bite Block: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Twisted Annealed Wire for Binding]

China OEM ABS Clear Plastic Injection Molding, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Widely Use 32 Cavities Pet Preform Water Bottle Mould-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse]

China OEM ABS Clear Plastic Injection Molding,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine]

China OEM ABS Clear Plastic Injection Molding, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Black Powder Coated Steel Sheet Metal Housing, Part I.Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Reflective Tape for Vehicle Reflective Markings (tape-02)]

Whitaker,J.R.(1963),Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape. Analytical Chemistry35(12), 1950-1953[Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby TrolleyAuthor for correspondence