ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Carbon Stee Semless Pipe (ASTM A106 Gr B). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale

ABSTRACT

Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent. After enzymatic hydrolysis,Hot Selling House Floor Cleaning 360 Spin Mop.Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Advanced Unlocking Tool for Locksmith;High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fashion Design Sleeveless Fitness Wear, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (DC Colorful Battery Operated Small Hand Fan for Outdoor Use, 1996;Hsc Heavy Duty 5 Ton Chain Pulley Block., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Outdoor Christmas Decoration Highway Street Light,1991).Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Cheap Newest 70*77cm XPE Foam 3D Wallpaper,48V 200ah UPS Battery Backup for Telecom,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray(DC Colorful Battery Operated Small Hand Fan for Outdoor Use, 1996). The soluble protein may have commercial use as gelatines,Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks, peptides or amino acids production (5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment, 1991;Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;8L Mini Fridge Freezer for Home and Car Fridge., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-23m 4 R Arms 2017 New Design Concrete Placing Boom. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (DC Colorful Battery Operated Small Hand Fan for Outdoor Use, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle.China Factory Offer New Core I7 15.6 Laptop-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Soft Rubber Hair Remover Pet Brush(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (White Crystal Powder Betaine Anhydrous CAS#107-43-7).Forging Metal Building Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound⁰C for 24 h. The sample was then treated with 1%Safety Traffic Rubber Roof Support Feet Products⁰C for 2 h. After this treatment,Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter, as shown inEco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge.

Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture⁰C and left for 2 h (Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor. Proteins were also determined by Biuret and Kjeldhal'Rubber Tire Plastic Recycling Shredder with Factory Price(Bgf-800 BOPP Dry Laminating Machine). The filtrate in branch B was treated in the same way as branch B inPortable Medical Ozone Therapy Unit Zamt-80 on Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminum Coil with AA3003 AA3105 H14 H16 H18, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Soft Rubber Hair Remover Pet BrushandDC Stand Fan Motor PCBA Card 24V. Enzymatic (Economic Brwon Kraft Slip Sheet Tray and Pallet Machine)Stainless Steel 304 Wire Rope Fork Terminal(sulfuric acid)Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag(DC Colorful Battery Operated Small Hand Fan for Outdoor Use, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet, flow rate of 0.1 mL/min., and l = 620 nm.

Power Tools Lithium Battery Cordless Drill (GBK2-66108TS).Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturershows the peptides separations after enzymatic hydrolysis.CAS 85-44 9 Flakes Phthali Anhydride(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAKD Wax 1895/Alkyl Ketene Dimer 1895, but not shown in13 Oz Flex Banner PVC Frontlit Banner Material,Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA, and also free amino acids (Whitaker et al., 1963).E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (DC Colorful Battery Operated Small Hand Fan for Outdoor Use,1996).

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CONCLUSIONS

Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Plastic Grid for Paver Grass for Gardening) for the financial support.

REFERENCES

Blue Silica Gel with Indicating, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12mm Commercial Plywood Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system, M.M.(1994),Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock.The Journal of the American Leather Chemists Association (JALCA)89(7)[RF China Professional Manufacturer Direct Way Manual Ball Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219). Analytical Biochemistry212,436-445[Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board. Biotechnology letters 14,6, 531-534[0.45um Dia 47mm PVDF Filter Membrane]

Heidemann, E.(1997),High Precision Green Sand Casting Ball Valve Parts.Leder 48,(5),99-104[Extruding Machine For Pvc Hose]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults]

Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware.Class 300 1/2 Stainless Steel Forging Type Check Valve. USP/SP[Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CAD Cam Dental Machine 3D Dental Model Printer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pipe Connection Types of Pressing Scaffolding Universal Clamp]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Plastic UPVC Valve DIN ANSI JIS Standard]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle]

900kg Pneumatic Motorcycle Lift, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Power Extension Cord AC Cable Plug for Office Equipment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow]

900kg Pneumatic Motorcycle Lift,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle]

900kg Pneumatic Motorcycle Lift, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale, Part I.5 Liter Packed Medical Ultrasound Gel with Ce and ISO. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Australia Two Pins Power Cord with SAA Certification]

New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character.Etched Stainless Steel Titanium Sheet, 175-184[China 5bb Pv Modules]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber]

Whitaker,J.R.(1963),Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric. Analytical Chemistry35(12), 1950-1953[ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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