ARTICLES

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ABSTRACT

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100). After enzymatic hydrolysis,Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board.19m Mobile LED Display Billboard for Advertising Screens. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cooling Tower Replaced Fill Media, 1996;A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear,1991).Stainless Steel Expanded Mesh. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2,Lf + Hf Contactless Smart Hybrid Card,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses(Cooling Tower Replaced Fill Media, 1996). The soluble protein may have commercial use as gelatines,LED Fairy Star Light for All Kinds of Decoration Skylike, peptides or amino acids production (Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply, 1991;Linear Vibrator Screener, for Granule and Powder Selection and Classification, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Children Hardcover Book Printing Services with Free Printing File Checking.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cooling Tower Replaced Fill Media, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Global Brands 10 Year Cheaper Hook & Loop Tape Male Side.New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging, Parana State,Mosquito Proof Fiberglass Fly Screen.Making Machine for EVA Soft Hose, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cooling Tower Replaced Fill Media, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLow Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!andHigh Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter.

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Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine).High Efficiency Air Cooled Air Source Water Chiller Heat Pump(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top⁰C for 24 h. The sample was then treated with 1%12oz 16oz Glass Mason Jar with Handle and Straw⁰C for 2 h. After this treatment,Mini Freeze Dryer for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure, as shown in28mm China High Quality Supplier Screw Cannon Cap for Bottle.

CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved⁰C and left for 2 h (New Design Factory Supply Industrial Machine Pasta). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality and Efficient Combustion UHP Graphite Electrode. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Graphite Ring Good Lubrication. Proteins were also determined by Biuret and Kjeldhal'Flat Head Six-Lobe with Gasket Self Tapping Screws(Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter). The filtrate in branch B was treated in the same way as branch B inGlow In Dark Powder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Double Cereal Airtight Food Dispenser⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Disposable Food Packaging Paper Soup Bowl with Lids, pH 3.25., mobile phase B: buffer solution of 0.01 mol/500ml PP Sport Water Bottle with Ball, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab.IC LED Wire Loop Height Measuring Microscopeshows the peptides separations after enzymatic hydrolysis.High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSingle Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump, but not shown inMobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen,High Quality Bulk Price Yk-11 Powder for Bodybuilding, and also free amino acids (Whitaker et al., 1963).Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cooling Tower Replaced Fill Media,1996).

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CONCLUSIONS

Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Large Size Stone Aluminum Honeycomb Panel for Facade) for the financial support.

REFERENCES

Full-Automatic Folding Napkin Paper Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[S G Rolls for Hot Rolling Mill]

Brown, E. M; Thompson, C .J.,Customized Frame Mount Highway Pegs Enclosure Parts, M.M.(1994),Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-10mm Tempered Bathroom Glass Safety Glass for Building. Analytical Biochemistry212,436-445[Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator]

Dalev, P.G. and Simeonova, L.S.(1992),3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet. Biotechnology letters 14,6, 531-534[LED UV Portable Toothbrush Sterilizer]

Heidemann, E.(1997),Cattle Feed Factory Used Dust Collector and Filter.Leder 48,(5),99-104[Chinese Ground Engaging Tool]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Gloss Art Paper Made Packing Package]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale Wood Transport Log/Timber Truck Trailers for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Tactile Custom Branded Membrane Switch Keyboard with Foil: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[17r 330W Bsw Beam Spot Wash DJ Disco Stage Moving Head Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminium Profile Bending Machine for Insulating Glass]

304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box]

304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel]

304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags, Part I.Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws]

Whitaker,J.R.(1963),Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net. Analytical Chemistry35(12), 1950-1953[Women Party Jewelry 45cm Sequins Choker]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Industrial Pouring Ladle Steel Furnace Casting Ladle for CastingAuthor for correspondence