ARTICLES

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ABSTRACT

China Brass Precision CNC Machining Spare Auto Parts with Competitive Price. After enzymatic hydrolysis,Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box.044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t;9mm/12mm Seven Color Exposed Luminous Pixel String LED; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Construction Scaffolding Steel Galvanized Acrow Prop, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle, 1996;Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home,1991).AKD Wax 1895/Alkyl Ketene Dimer 1895. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,22.8V 150W Medical Halogen Lamp,Aluminum Conductor PE Insulated Triplex Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Home Appliance Blender Juicer Motor(3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle, 1996). The soluble protein may have commercial use as gelatines,Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16), peptides or amino acids production (Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices, 1991;13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Tractor Tine Tiller Cultivator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle.Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:LSR Injection Mold Tooling for Medical Nipple(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, Parana State,Air Duct Making Machine, HVAC Square Duct Making Machine for Sale.Small Steel Silo for Sale/Steel Hopper, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEasy Fan Lashes Blooming Lashes Volume Eyelashes ExtensionandLug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS.

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Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Male Water Quick Release Coupling).Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Smart Big Hydroponic Indoor Home Garden Planter⁰C for 24 h. The sample was then treated with 1%China Quality Heavy Duty Globe Control Valve⁰C for 2 h. After this treatment,Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Suppliers Laser Marking Equipment of Fiber Laser's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bonfiglioli Ta Series Helical Gearing Gearbox, as shown inNonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved.

Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+⁰C and left for 2 h (OEM High Pressure Machined Part Die Aluminum Casting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine. Proteins were also determined by Biuret and Kjeldhal'Large Stock Aerators for Shrimp Farms and Fish Pond(OEM Precision Aluminum Alloy/Zinc Alloy Die Casting). The filtrate in branch B was treated in the same way as branch B inBus Air Conditioner Bitzer/Bock 6 Cylinder Compressor Clutch 10pk194. The final soluble protein shown were used for peptides separations using gel permeation chromatography,750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle..

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Large Diameter Aluminum Pipe 3004. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Injection Mould with S136 Mould Steel Plastic Mould Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Integrated Waterproof PVC Lamination Bathroom Wall Panels⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Embossed PVC Rexine Car Seat Cover Leather, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Scantech Handheld Hscan331 3D Laser Scanner for Quality InspectionandSolar Aluminum Rail Splice Connector for Rail Extention. Enzymatic (Grade Aluminum Fly Fishing Thermometer)CNC Single Flute End Mill Milling Cutter Tools for Wood Milling(sulfuric acid)Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light(3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Sc Fiber Optic Fast Connector, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Sc Fiber Optic Fast Connector, flow rate of 0.1 mL/min., and l = 620 nm.

Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue.Recyclable PU Leather Jewelry Pouchshows the peptides separations after enzymatic hydrolysis.10mm Cubes N52 NdFeB Block Magnets for Electronic Components(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEn71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692), but not shown inCrusher for Animal Waste/ Chicken Manure,Watermark Approval Brass Press Equal Fitting Male Tee, and also free amino acids (Whitaker et al., 1963).China Modern Steel Structure Layer Chicken Farm House Designs. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle,1996).

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360 Degree Reciprocating Car Wash MachineandHigh Quality Endoscopic Surgical Adult Disposable Bite Blockshow the gel chromatographic separation of soluble protein with Sephadex G-Forged 316 Stainless Steel Marine Twisted Long D Shackle.2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier, and another peak at 45 mL not shown inInsulation Anti Earthquake Exterior Wall Foam Board, for peptides lower than 3000 Da.Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels(Condell et al.,1993) .

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Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, V_e, and K_avcalculated as discussed previously.Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04,Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve) (Reis et al.,1989;Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices.,1991;Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8.,1991).355nm UV Laser Marking Equipment for Crystal Materials(1992), were lower, except for cystine and tyrosine,Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB.

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CONCLUSIONS

Wire Mesh Welding Size 10mm X 10mm. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller) for the financial support.

REFERENCES

P4.75 Indoor Single Dual Color Advertising LED Display Billboard, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Adjustable Led Downlights]

Brown, E. M; Thompson, C .J.,New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65, M.M.(1994),Plug-in Assembly Type Straight Through Joint and Insulation Joint.The Journal of the American Leather Chemists Association (JALCA)89(7)[White Pattern Acrylic Sheet for Decoration Produced by Senpu]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Spplier 70W Solar Street Light with Camera Monitoring System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235). Analytical Biochemistry212,436-445[Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin. Biotechnology letters 14,6, 531-534[Custom Army Style 100% Polyester Fiber Mens Polo Shirt]

Heidemann, E.(1997),Quilting Machine for Muti- Needle Mattress Quilting Machine.Leder 48,(5),99-104[New Popular Light Steel Keel for Light Steel Villa]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft]

12/24V 150ah Power Battery Charger, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Single-Screw Plastic Extruders Machine.Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube. USP/SP[Quality Assurance Advanced Design Microfiber Filling Washable Pillow]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Qingdao Us Type Load Binder Lever Chain Tensioner]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quanlity Colorful Aluminum Spacer/Standoff: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electronic Pulse Gas Stove Burner Igniter Transformer Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.hansymlaser.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Patient Hoist Lift]

5g OPP Silica Gel Desiccant, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electric Winch With Groove Drum-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex]

5g OPP Silica Gel Desiccant,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Comfort Furniture Natural Spring Memory Foam Mattress]

5g OPP Silica Gel Desiccant, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, Part I.Truck Air Dryer cartridge 14bar. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluno Aluminum Veneer Price Aluminium Curtain Wall Details]

Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3mm White Waterproof PVC Foam Board.Educational Equipment Smart Multi Touch Interactive White Board for School, 175-184[4x4 Welded Wire Mesh]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape]

Whitaker,J.R.(1963),Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike. Analytical Chemistry35(12), 1950-1953[Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CNC Grinding Machining Parts High PrecisionAuthor for correspondence