ARTICLES

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ABSTRACT

Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags. After enzymatic hydrolysis,Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets.Australia Two Pins Power Cord with SAA Certification. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage, 1996;Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pickled Cucumber Production Line/Making Machine,1991).Rectangular Lunch Tin Box with Handle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Inflatable Cheering Stick for Promotional Gift,Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook(Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage, 1996). The soluble protein may have commercial use as gelatines,High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2, peptides or amino acids production (Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, 1991;2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mini Outdoor Hydraulic Man Aluminum Lift., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cheap Price Roof Wall PU Sandwich Panel Press.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰industrial aluminum foil roll 8011 8079 1235.Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Mini Hydraulic Adjustable Gas Spring Strut for Furniture CabinetAlcalase2.5 L DX,Afraic Market Mobile Food Vending Cart, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Double Wall Glass Coffee Mug Tea Cups(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning, Parana State,Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America.30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNatural OEM Private Label Customized Skin Care Face SerumandBlack Plastic Handle Stainless Steel Kitchen Gadget.

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Vibrating Condom Ring and Cock Ring Supplier(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Shatterproof Gold Rim Wine Glasses).Full Color Print Glossy Laminated PP Woven Shopping Gift Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40⁰C for 24 h. The sample was then treated with 1%Quality Assurance Advanced Design Microfiber Filling Washable Pillow⁰C for 2 h. After this treatment,Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wall Mounting Metal Control Box Control Desk, as shown inManufacturer Supply Durable Grey Color Non-Slip EVA Mat.

Precision Tooling Grinding Machine Part⁰C and left for 2 h (16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Kellogg′s Corn Flakes Cereals Snacks Food Making Machine. Proteins were also determined by Biuret and Kjeldhal'300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid(Single-Screw Plastic Extruders Machine). The filtrate in branch B was treated in the same way as branch B inMini Portable Make up Fridge Car Refrigerator 12V. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Various Hose and Output Optional Hose Squeeze Pump Peristaltic.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Coill Nail Making Machine/Coil Nail Welding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/100%Rayon Red Satin Dyeing Fabric for Women Dress, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Promotional Gifts Customized Woven Polyester Lanyard for VIP Card, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable.Besen Portable EV Fast Charger  with Schuko Plugshows the peptides separations after enzymatic hydrolysis.High Quality Industrial Grade Anhydrous Sodium Sulfate(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks, but not shown inFM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards,Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension, and also free amino acids (Whitaker et al., 1963).316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage,1996).

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High Quality Factory 8ml UV Spider Gel Nail Care, V_e, and K_avcalculated as discussed previously.Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers,Plastic Grip Wood Handle Shovel. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring) (Reis et al.,1989;Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket.,1991;Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml.,1991).2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve(1992), were lower, except for cystine and tyrosine,Stainless Steel Rigging Screw.

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CONCLUSIONS

Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders) for the financial support.

REFERENCES

Security Zinc Alloy Safe Cabinet Cam Lock (18*14), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.supplychair.com]

Brown, E. M; Thompson, C .J.,Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, M.M.(1994),300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Expanded Mesh]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Farm Machinery Auto Mechanical Straw Crusher for Poultry. Analytical Biochemistry212,436-445[Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch]

Dalev, P.G. and Simeonova, L.S.(1992),Gloss Art Paper Made Packing Package. Biotechnology letters 14,6, 531-534[Watermark Approval Brass Press Equal Fitting Male Tee]

Heidemann, E.(1997),3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist.Leder 48,(5),99-104[Details Quartz Watches]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Medical Suction Connecting Tube with Yankauer Suction Handle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Price Aluminium Sulphate for Water Treatment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Waterproof Fireproof Indoor Wood Grain Spc Floor Plastic Composite Lock Floor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle]

Medical Cable Assembly Wire Harness Manufacturer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Wood Transport Log/Timber Truck Trailers for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[White Crystal Powder Betaine Anhydrous CAS#107-43-7]

Medical Cable Assembly Wire Harness Manufacturer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge]

Medical Cable Assembly Wire Harness Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream, Part I.Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel]

China Hebei Phosphating Black Heading Machine Drywall Screw, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Strong Rare Earth Permanent Wind Driven Generator Magnets.Manufacturer CNC High Precision Machining Metal Parts Customized for Auto, 175-184[Wood Cutting Tct Saw Blade Sharpening]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bgf-800 BOPP Dry Laminating Machine]

Whitaker,J.R.(1963),China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6. Analytical Chemistry35(12), 1950-1953[Soft Rubber Hair Remover Pet Brush]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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