ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Online High Sensitivity Checkweigher with Pusher Bar. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Piggery Farm Pig/Sow Gestation Equipment From China Factory

ABSTRACT

Lw250*700 Spiral Discharge Decanter Separator for Water Treatment. After enzymatic hydrolysis,Single Cheap Custom Festival Woven RFID Fabric Wristband for Events.Children Gift Outdoor Wooden Rocking Horse Toy. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330;TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, 1996;China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe,1991).Outdoor High Neck Kids Boots Casual Rubber Men Work Boots. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061),Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet(ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, 1996). The soluble protein may have commercial use as gelatines,CNC Grinding Machining Parts High Precision, peptides or amino acids production (High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, 1991;Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Disposable Clear Take Away Plastic Salad Bowl Forming Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP.Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Carbon Stee Semless Pipe (ASTM A106 Gr B)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Advanced Unlocking Tool for Locksmith, Parana State,Piggery Farm Pig/Sow Gestation Equipment From China Factory.High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Walland2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon.

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Fashion Design Sleeveless Fitness Wear(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Single Cheap Custom Festival Woven RFID Fabric Wristband for Events).DC Colorful Battery Operated Small Hand Fan for Outdoor Use(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hsc Heavy Duty 5 Ton Chain Pulley Block⁰C for 24 h. The sample was then treated with 1%Outdoor Christmas Decoration Highway Street Light⁰C for 2 h. After this treatment,Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Cheap Newest 70*77cm XPE Foam 3D Wallpaper's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)48V 200ah UPS Battery Backup for Telecom, as shown inBulk Wonderful Buckle Sexy Bikini with Belt Swimwear.

450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray⁰C and left for 2 h (White Melamine Powder for Industrial Uses). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment. Proteins were also determined by Biuret and Kjeldhal'Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms(Air Duct Making Machine). The filtrate in branch B was treated in the same way as branch B inHot Selling House Floor Cleaning 360 Spin Mop. The final soluble protein shown were used for peptides separations using gel permeation chromatography,8L Mini Fridge Freezer for Home and Car Fridge.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av23m 4 R Arms 2017 New Design Concrete Placing Boom(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Factory Offer New Core I7 15.6 Laptop⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mining Machinery Parts Diamond Saw Blade Segments for Marble, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Soft Rubber Hair Remover Pet Brush.Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipmentshows the peptides separations after enzymatic hydrolysis.Forging Metal Building Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWorkshop Tools 7.5 Ton Electric Winch with Dual Speed, but not shown inJumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue,Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound, and also free amino acids (Whitaker et al., 1963).Safety Traffic Rubber Roof Support Feet Products. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L,1996).

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CONCLUSIONS

Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Selling House Floor Cleaning 360 Spin Mop) for the financial support.

REFERENCES

Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Biometric Machine Factory & Manufacturer]

Brown, E. M; Thompson, C .J.,Industrial Mens Cotton Reflective Tape Work Pants, M.M.(1994),Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye.The Journal of the American Leather Chemists Association (JALCA)89(7)[Raw Material Adhesive NBR Latex of Latex Long Gloves]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Precision Diamond Wire Saw Rope for Cutting Concrete Stone]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminum Coil with AA3003 AA3105 H14 H16 H18. Analytical Biochemistry212,436-445[Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB. Biotechnology letters 14,6, 531-534[HDPE Auto-Deflashing Plastic Bottle Blowing Machine]

Heidemann, E.(1997),DC Stand Fan Motor PCBA Card 24V.Leder 48,(5),99-104[Sode Lime Glass Tubular Small Bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment]

Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Economic Brwon Kraft Slip Sheet Tray and Pallet Machine.Stainless Steel 304 Wire Rope Fork Terminal. USP/SP[Small Steel Silo for Sale/Steel Hopper]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[for Bahrain Market Hot Cafe Vending Machine F303V]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[TPU TPR Rubber Band Plastic Extruder Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fish Oil Capsules]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Warm Paste Packaging Machine]

Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2000LTR Horizontal Foam Tank Fire Fighting Suppression]

Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Upper Limbs Machine/Spare Parts for Fitness Equipment]

Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CAS 85-44 9 Flakes Phthali Anhydride, Part I.Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration]

E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Good Design Custom Made Cheap Recycle Paper Bags.Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White, 175-184[Fastener JIS Forged Steel Frame Type Turnbuckles (Hook and Eye)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ 13947]

Whitaker,J.R.(1963),Non-Ionic Polyacrylamide Flocculant. Analytical Chemistry35(12), 1950-1953[External Wall Material Panels/Aluminum Composite Sheet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass FabricAuthor for correspondence