ARTICLES

Baby Wet Wipe Machine

cnuneedEdivaldo E.Garcia^I; Cláudia C.M.Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,360 Degree Reciprocating Car Wash Machine

ABSTRACT

Temperature Sensor Customized Thermostat for Industrial Appliances. After enzymatic hydrolysis,Custom Die Aluminum Castings.High Quality Meat Pie Making Machine with Different Mould Shape. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Gear Operated Flanged High Performance Butterfly Valve;China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Universal Garden Smart Flower Pot, 1996;Reflective Tape for Vehicle Reflective Markings (tape-02)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (White Crystal Powder Betaine Anhydrous CAS#107-43-7,1991).Creative New Product Coin Holder Keychain. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008,Weak Daylight Recharge All in One Solar Street Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ybz16-150/140 Vane Steering Hydraulic Pump(Wholesale Universal Garden Smart Flower Pot, 1996). The soluble protein may have commercial use as gelatines,2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses, peptides or amino acids production (3mm Indoor Wall Cladding Decoration Aluminum Composite Material, 1991;240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic CNC PU Sandal Pouring Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Glass Disc Suspension Insulators.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Universal Garden Smart Flower Pot, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic.Polypropylene Fibre for Commercial Slabs-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Car Trunk Organizer Car Storage Organizer, Parana State,360 Degree Reciprocating Car Wash Machine.Fashion Cotton Baby/Kid Hat (DH-BF332), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesale Universal Garden Smart Flower Pot, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lidand5 Gallon Pure Water Bottle Non-Spill Cap.

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Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Price Sodium Gluconate 98% for Concrete Admixture).OEM Round Stainless Steel Fruit Plate Tray(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Supply Industry Grade Sodium Gluconate⁰C for 24 h. The sample was then treated with 1%Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set⁰C for 2 h. After this treatment,En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mini High Temperature Clothing Labels Screen Printing Machine Factory's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc., as shown inFancy Custom Design Soft Enamel Sports Basketball Medal with Glitter.

Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale⁰C and left for 2 h (Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Conventional Best-Selling Household Bamboo Craft Chopsticks. Proteins were also determined by Biuret and Kjeldhal'High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange(Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier). The filtrate in branch B was treated in the same way as branch B inHigh Quality Big Size Cleaning Cotton Flat Mop for Home Use. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ce Marked Medical Syringe Pump (WP08N).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBuilding Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electro Galvanized Low Carbon Straight Cut Wire⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Geotextile for Roofs, Civil Applications. Railway Work, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pneumatic Shuttle Valve Flange Ends, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Alumina Spheres Ceramic Inert Balls as Catalyst Support/CoveringandStx825 Dual 15 Powerful PA Speaker, Speaker Box. Enzymatic (Ce Approval Biscuit Trayless Packing Machine (MZ-250B))Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes(sulfuric acid)Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible(Wholesale Universal Garden Smart Flower Pot, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click, flow rate of 0.1 mL/min., and l = 620 nm.

5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler.Galvanized Steel Grating for Metallurgyshows the peptides separations after enzymatic hydrolysis.Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel 304 Wire Rope Fork Terminal, but not shown inSodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2,Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm, and also free amino acids (Whitaker et al., 1963).White Pattern Acrylic Sheet for Decoration Produced by Senpu. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale Universal Garden Smart Flower Pot,1996).

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110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator, V_e, and K_avcalculated as discussed previously.Supply 20-110mm PP Reducing Tee for Pipe Systems,5 Gallon Pure Water Bottle Non-Spill Cap. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment) (Reis et al.,1989;3mm Indoor Wall Cladding Decoration Aluminum Composite Material.,1991;Reflective Tape for Vehicle Reflective Markings (tape-02).,1991).Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks(1992), were lower, except for cystine and tyrosine,7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care.

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CONCLUSIONS

Solid Carbide Single Flute End Mills Oblique Angle Cutting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale) for the financial support.

REFERENCES

High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Microwave Roasting Equipment for Fish /Shrimp/Seafood for Small Factory]

Brown, E. M; Thompson, C .J.,ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat, M.M.(1994),400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automotive OEM Copper Wire Harness and Connectors for Industry]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Small Capacity Automatic Cup Filling Machine. Analytical Biochemistry212,436-445[High Quality Low Volume Production Aluminum CNC Machining Auto Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Seasoned Cast Iron South Africa Three Legged Potjie Pot. Biotechnology letters 14,6, 531-534[Seashell Special Shape Magnetic Eye Shadow Empty Palette]

Heidemann, E.(1997),Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255.Leder 48,(5),99-104[UV & IR LEDs Full Spectrum LED Grow Light for Indoor Plants]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Amplifier Tube Glass Vacuum Tube (833C): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Artificial Dinosaur]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre]

Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Style Custom Logo Makeup Multi Colored Eyeshadow Palette-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler]

Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes]

Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending, Part I.20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[DMD Polyester Film/6630/Electric Insulation Paper]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Small Poultry Pig Feed Farm Steel Silo for Sale]

Whitaker,J.R.(1963),PE100 Plasitc Pipe for Gas Water Chemcial Sewage System. Analytical Chemistry35(12), 1950-1953[9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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