ARTICLES

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ABSTRACT

3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts. After enzymatic hydrolysis,Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone.China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Price Aluminium Sulphate for Water Treatment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Loading Truck with Cummins Engine, 1996;Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (50kg Soya Bean Seed PP Woven Bags Sacks,1991).Iron Wire Dry Type Straight Line Wire Drawing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile,Barricade Tape,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip(Hot Sale Loading Truck with Cummins Engine, 1996). The soluble protein may have commercial use as gelatines,Fully Automatic Industrial Microwave Dehydrator, peptides or amino acids production (Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L), 1991;Oral Thermometer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wood Fireplace Double Skin Chimney Flue Pipes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Loading Truck with Cummins Engine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Sensitivity Ground Metal Detector Searching for Treasure.Aluminium Profile Bending Machine for Insulating Glass-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Plastic Mould Dongguan Molding Factory(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper).OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004⁰C for 24 h. The sample was then treated with 1%Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer⁰C for 2 h. After this treatment,PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Good Quality Banded Type Tee's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls, as shown inHydraulic Three Roll Mill with Hydraulic Squeeze Pressing system.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%33kv 100kVA Dry-Type Distribution Transformer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFruit/ Vegetable Double Spray High Pressure Cleaner for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc , pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards) for the financial support.

REFERENCES

Automotive Sheet Metal Stamping Car Auto Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Muffin Box Packaging Jewelry Packaging Box Cardboard]

Brown, E. M; Thompson, C .J.,Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control, M.M.(1994),Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Fabric]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1. Analytical Biochemistry212,436-445[Blince High Torque OMR Hydro Motor for Injection Moulding Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation. Biotechnology letters 14,6, 531-534[Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Nice Quality Coloring Printing Children Board Book Printing]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mini Double Hole ABS Plastic CNC Machined Parts Importer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[12oz 16oz Glass Mason Jar with Handle and Straw]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Portable Air Disinfector 3.5g-7g with UV Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy]

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Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Underground Carport]

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Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Bucket Segment Bolt 6V0937.Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture, 175-184[Promotional School Lunch Bag Children′s Tote Cooler Bag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Commecial Use Watch Display Cabinet for Watch Store Interior Design]

Whitaker,J.R.(1963),Outdoor Server Rack Network Data Cabinet. Analytical Chemistry35(12), 1950-1953[Ce Marked Medical Syringe Pump (WP08N)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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