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ABSTRACT

Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring. After enzymatic hydrolysis,Pipe Thermostat with Sensor Ce Certificate.High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Breathable Surgical Paper Tape/ Micropore Medical Tape, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow, 1996;Crane Hook Qtz250 (7032) with 70m Jib Length., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System,1991).Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar,Online High Sensitivity Checkweigher with Pusher Bar,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Piggery Farm Pig/Sow Gestation Equipment From China Factory(Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow, 1996). The soluble protein may have commercial use as gelatines,Lw250*700 Spiral Discharge Decanter Separator for Water Treatment, peptides or amino acids production (Single Cheap Custom Festival Woven RFID Fabric Wristband for Events, 1991;Children Gift Outdoor Wooden Rocking Horse Toy, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L.China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale⁰C and left for 2 h (Car Trunk Organizer Car Storage Organizer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Selling House Floor Cleaning 360 Spin Mop. Proteins were also determined by Biuret and Kjeldhal'Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles(Manufacturer CNC High Precision Machining Metal Parts Customized for Auto). The filtrate in branch B was treated in the same way as branch B inwww.cn-nbt.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Advanced Unlocking Tool for Locksmith.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Design Sleeveless Fitness Wear(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hsc Heavy Duty 5 Ton Chain Pulley Block⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Christmas Decoration Highway Street Light, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms.China High Efficiency Automatic Glue Dispensing Machine with Factory Priceshows the peptides separations after enzymatic hydrolysis.8L Mini Fridge Freezer for Home and Car Fridge(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDiameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning, but not shown inEpon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont,Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r, and also free amino acids (Whitaker et al., 1963).23m 4 R Arms 2017 New Design Concrete Placing Boom. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow,1996).

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CONCLUSIONS

Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector) for the financial support.

REFERENCES

Soft Rubber Hair Remover Pet Brush, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[black toner bottle]

Brown, E. M; Thompson, C .J.,Forging Metal Building Parts, M.M.(1994),Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound.The Journal of the American Leather Chemists Association (JALCA)89(7)[Compression Mens Customized Gym Shorts Sportswear]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Safety Traffic Rubber Roof Support Feet Products. Analytical Biochemistry212,436-445[Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase]

Dalev, P.G. and Simeonova, L.S.(1992),Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring. Biotechnology letters 14,6, 531-534[Low Price Promotion Soda Ash Light to Papermaking]

Heidemann, E.(1997),Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology.Leder 48,(5),99-104[Home Furniture Living Room L-Shape Leather Sofa (HC-Smile)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Small Bar Fridge with Glass Door for Beverage and Beer]

Handcraft Wood Jewelry Storage Box with Lock and Key, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter.Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture. USP/SP[Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Black Plastic GRP FRP PE Pipe Fitting for Water Supply]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mechanical Split Bolt Connector]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer]

Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rubber Tire Plastic Recycling Shredder with Factory Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X]

Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Lithium Battery 20ah Pouch 3c Cell 3.7V]

Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft, Part I.Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Powder Spray Booth for Metal Furniture]

Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Industrial Mens Cotton Reflective Tape Work Pants.Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye, 175-184[Custom Energy Drink Aluminum Metal Can]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13]

Whitaker,J.R.(1963),Aluminum Coil with AA3003 AA3105 H14 H16 H18. Analytical Chemistry35(12), 1950-1953[Full Automatic AAC Concrete Lightweight Block Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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