ARTICLES

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ABSTRACT

Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet. After enzymatic hydrolysis,Afraic Market Mobile Food Vending Cart.Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Double Wall Glass Coffee Mug Tea Cups;Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Vibrating Condom Ring and Cock Ring Supplier, 1996;Full Color Print Glossy Laminated PP Woven Shopping Gift Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40,1991).Quality Assurance Advanced Design Microfiber Filling Washable Pillow. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price,Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wall Mounting Metal Control Box Control Desk(Vibrating Condom Ring and Cock Ring Supplier, 1996). The soluble protein may have commercial use as gelatines,Precision Tooling Grinding Machine Part, peptides or amino acids production (Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks, 1991;Kellogg′s Corn Flakes Cereals Snacks Food Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Various Hose and Output Optional Hose Squeeze Pump Peristaltic.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Coill Nail Making Machine/Coil Nail Welding Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Vibrating Condom Ring and Cock Ring Supplier, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet.2017 Hot Diped Galvanized Welded Gabion Basket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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HDPE Auto-Deflashing Plastic Bottle Blowing Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price).Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Industrial Grade Anhydrous Sodium Sulfate⁰C for 24 h. The sample was then treated with 1%Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension⁰C for 2 h. After this treatment,316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Low Cost Prefab Steel Poultry Chicken House on Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Blue Color Fish Paper/6520/6521 Flexible Composites, as shown in8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Rigging Screw. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPolycarbonate Hollow Sheet UV Protected with 10 Years Warranty(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bubule 17 Inch PP Carry on Kids Travel Luggage for Children⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Farm Machinery Auto Mechanical Straw Crusher for Poultry, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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22.8V 150W Medical Halogen LampandHigh Quality Screw Tools Tungsten Carbide Shaped Cold Heading Diesshow the gel chromatographic separation of soluble protein with Sephadex G-China Hebei Phosphating Black Heading Machine Drywall Screw.Strong Rare Earth Permanent Wind Driven Generator Magnets, and another peak at 45 mL not shown inVarious Hose and Output Optional Hose Squeeze Pump Peristaltic, for peptides lower than 3000 Da.Manufacturer CNC High Precision Machining Metal Parts Customized for Auto(Condell et al.,1993) .

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CONCLUSIONS

Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Density Artificial Grass Football Playground Surfaces (STO)) for the financial support.

REFERENCES

100% PVC Resin PVC Clear Transparent Film for Shower Curtain, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[15 Ton Excavator XCMG Xe150d Excavator for Sale]

Brown, E. M; Thompson, C .J.,Hotel Security Improvement Zinc Alloy Door Chain, M.M.(1994),Anti-Pollution Type 160kn Insulator Porclelain Disc.The Journal of the American Leather Chemists Association (JALCA)89(7)[Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nickel 200 Floating Ball Valve. Analytical Biochemistry212,436-445[Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring]

Dalev, P.G. and Simeonova, L.S.(1992),3-Layer PE Coating Production Line. Biotechnology letters 14,6, 531-534[Aluminum Coil with AA3003 AA3105 H14 H16 H18]

Heidemann, E.(1997),Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase.Leder 48,(5),99-104[Multi Bag Filter Water Treatment System]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plush Comfort Spring Mattress Cheap Price for Wholesale]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wooden Queen/King Size Japan Sofa Bed G1162: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Move easily portable Double Arm for two work station Free Standing Exhaust Pure-Air PA-300TD-IQ Dust Filtration System Nail with CE Certification]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Factory Organic Ethyl Ascorbic Acid]

J55 Short Round Thread Seamless Casing Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube]

J55 Short Round Thread Seamless Casing Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women]

J55 Short Round Thread Seamless Casing Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube, Part I.Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter]

Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation.Dust Hood Fume Extraction Systems for Robotic Laser Cutting, 175-184[Professional Classic Cat Litter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[10mm Cubes N52 NdFeB Block Magnets for Electronic Components]

Whitaker,J.R.(1963),IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector. Analytical Chemistry35(12), 1950-1953[High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel CF8/CF8m Street Elbow Pipe FittingsAuthor for correspondence