ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Teflon for Industrial Plastic Gasket Seal with Good Quality. After enzymatic hydrolysis,Double Walled Stainless Steel Insulated Water Bottle.Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2018 Cheap PU Leather Bracelet in Alloy Jewelry;China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Competetive Price Tension Springs for Stapler, 1996;Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Men Casual Short Pants Custom Quality Running Sports Wear Shorts,1991).Horizontal Window Shade Blind Zebra Dual Roller Blinds Day. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Fashion Diaper Bags Mummy Bag,Mellow OEM Plastic Pet House Ml-HP004,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,12/24V 150ah Power Battery Charger(Competetive Price Tension Springs for Stapler, 1996). The soluble protein may have commercial use as gelatines,1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable), peptides or amino acids production (Air Duct Making Machine, HVAC Square Duct Making Machine for Sale, 1991;China Brass Precision CNC Machining Spare Auto Parts with Competitive Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Competetive Price Tension Springs for Stapler, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰9mm/12mm Seven Color Exposed Luminous Pixel String LED.Construction Scaffolding Steel Galvanized Acrow Prop-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Aluminum Conductor PE Insulated Triplex Cable, Parana State,Compact New Design IP65 Toggle Limit Switch.Home Appliance Blender Juicer Motor, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Competetive Price Tension Springs for Stapler, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePet Short Fiber Non Woven Geotextile for Landfill FiltrationandHigh Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire.

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Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Needle Rollers for Bearings).Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike⁰C for 24 h. The sample was then treated with 1%2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price⁰C for 2 h. After this treatment,Tractor Tine Tiller Cultivator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, as shown inAgricultural Mini Tiller Farm Wheel 62cc Cultivator.

Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics⁰C and left for 2 h (Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LSR Injection Mold Tooling for Medical Nipple. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Nail Stamping Plates Custom Metal Part Sheet Metal Stamping. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Sports Jacket Men Outdoor Waterproof Jackets(Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine). The filtrate in branch B was treated in the same way as branch B inPower Battery 48V 50ah LiFePO4 Battery Pack for UPS System. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Smart Big Hydroponic Indoor Home Garden Planter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady DressesandChina Quality Heavy Duty Globe Control Valve. Enzymatic (Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack)China Suppliers Laser Marking Equipment of Fiber Laser(sulfuric acid)Bonfiglioli Ta Series Helical Gearing Gearbox(Competetive Price Tension Springs for Stapler, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

CNC Single Flute End Mill Milling Cutter Tools for Wood Milling. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (AKD Wax 1895/Alkyl Ketene Dimer 1895) for the financial support.

REFERENCES

Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[P5 960*960mm LED Display Screen SMD Indoor /Outdoor]

Brown, E. M; Thompson, C .J.,Sc Fiber Optic Fast Connector, M.M.(1994),Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue.The Journal of the American Leather Chemists Association (JALCA)89(7)[Steel Stacking Restaurant Wedding Chair]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-10mm Cubes N52 NdFeB Block Magnets for Electronic Components. Analytical Biochemistry212,436-445[High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640]

Dalev, P.G. and Simeonova, L.S.(1992),Watermark Approval Brass Press Equal Fitting Male Tee. Biotechnology letters 14,6, 531-534[20 Experiences on Plastic Injection Moulding Service]

Heidemann, E.(1997),China Modern Steel Structure Layer Chicken Farm House Designs.Leder 48,(5),99-104[Building Materials Flexible Topcoat Wall Decorative Mortar HPMC]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Betaine Anhydrous with Best Price107-43-7]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steel Coil Trolley in Railway Transportation for Round Object]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle]

Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)]

Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator]

Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight, Part I.355nm UV Laser Marking Equipment for Crystal Materials. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LED UV Portable Toothbrush Sterilizer]

Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wire Mesh Welding Size 10mm X 10mm.P4.75 Indoor Single Dual Color Advertising LED Display Billboard, 175-184[Wholesale Price Sale Farming Rice Planter Machine Rice Seeder Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Gloss Art Paper Made Packing Package]

Whitaker,J.R.(1963),New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65. Analytical Chemistry35(12), 1950-1953[Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hot Sale Wood Transport Log/Timber Truck Trailers for SaleAuthor for correspondence