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ABSTRACT

Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure. After enzymatic hydrolysis,CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved.High Quality and Efficient Combustion UHP Graphite Electrode. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Graphite Ring Good Lubrication;Flat Head Six-Lobe with Gasket Self Tapping Screws; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile, 1996;0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Weiying Full Computerized Plain and Invisible Sock knitting Machine,1991).Hot Double Cereal Airtight Food Dispenser. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Disposable Food Packaging Paper Soup Bowl with Lids,500ml PP Sport Water Bottle with Ball,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stamping Fine Blanking Sheet Metal Fabrication Part(Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile, 1996). The soluble protein may have commercial use as gelatines,Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins, peptides or amino acids production (Trade Assurance Beautiful Silk Lace Closure, 1991;High Quality Needle Rollers for Bearings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heavy Duty Steel Warehouse Storage Pallet Rack., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Bulk Price Yk-11 Powder for Bodybuilding.Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery, Parana State,5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber.Fully Automatic Digital Pid Temperature Controller, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMug Printing Machine Heat Press Promotion BrazilandClosed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump.

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High Quality Anti-Corrosion Fiberglass Sheet and Roll(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight).Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Full-Automatic Folding Napkin Paper Machine⁰C for 24 h. The sample was then treated with 1%Customized Frame Mount Highway Pegs Enclosure Parts⁰C for 2 h. After this treatment,Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)10mm Tempered Bathroom Glass Safety Glass for Building's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet, as shown inLe1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter.

Cattle Feed Factory Used Dust Collector and Filter⁰C and left for 2 h (Colorful Electric English Funny Music Story Module Printing Book). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Group 152cm Picnic Folding in Half Table. Proteins were also determined by Biuret and Kjeldhal'Tactile Custom Branded Membrane Switch Keyboard with Foil(Ybz16-150/140 Vane Steering Hydraulic Pump). The filtrate in branch B was treated in the same way as branch B inFilament Acupuncture Non-Woven Geotextile. The final soluble protein shown were used for peptides separations using gel permeation chromatography,304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLuxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Australia Two Pins Power Cord with SAA Certification⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing EquipmentandPlastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net. Enzymatic (Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage)Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml(sulfuric acid)Pickled Cucumber Production Line/Making Machine(Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Rectangular Lunch Tin Box with Handle, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Rectangular Lunch Tin Box with Handle, flow rate of 0.1 mL/min., and l = 620 nm.

Inflatable Cheering Stick for Promotional Gift.Farm Machinery Auto Mechanical Straw Crusher for Poultryshows the peptides separations after enzymatic hydrolysis.Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPet Short Fiber Non Woven Geotextile for Landfill Filtration, but not shown inHigh Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire,Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook, and also free amino acids (Whitaker et al., 1963).High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile,1996).

Ferrous Sulphate Monohydrate -Feso4 H2O

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CONCLUSIONS

Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts) for the financial support.

REFERENCES

Afraic Market Mobile Food Vending Cart, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ups Battery Jars]

Brown, E. M; Thompson, C .J.,Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, M.M.(1994),Double Wall Glass Coffee Mug Tea Cups.The Journal of the American Leather Chemists Association (JALCA)89(7)[304 304L 904L 309S 310S Stainless Steel Design Checkered Plate]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning. Analytical Biochemistry212,436-445[28mm China High Quality Supplier Screw Cannon Cap for Bottle]

Dalev, P.G. and Simeonova, L.S.(1992),30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care. Biotechnology letters 14,6, 531-534[New Design Factory Supply Industrial Machine Pasta]

Heidemann, E.(1997),Vibrating Condom Ring and Cock Ring Supplier.Leder 48,(5),99-104[240v Led Light Bar]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[IC LED Wire Loop Height Measuring Microscope]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Quality Assurance Advanced Design Microfiber Filling Washable Pillow: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Basketball Court Floor Mat]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen]

Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Steel Stacking Restaurant Wedding Chair]

Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike]

Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wall Mounting Metal Control Box Control Desk, Part I.Precision Tooling Grinding Machine Part. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640]

Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Kellogg′s Corn Flakes Cereals Snacks Food Making Machine.300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid, 175-184[China Type-C Qc3.0 Charger]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[20 Experiences on Plastic Injection Moulding Service]

Whitaker,J.R.(1963),Various Hose and Output Optional Hose Squeeze Pump Peristaltic. Analytical Chemistry35(12), 1950-1953[Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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