ARTICLES

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ABSTRACT

SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends. After enzymatic hydrolysis,Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production.110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care, 1996;Solid Carbide Single Flute End Mills Oblique Angle Cutting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies,1991).ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films,Small Capacity Automatic Cup Filling Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Seasoned Cast Iron South Africa Three Legged Potjie Pot(7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care, 1996). The soluble protein may have commercial use as gelatines,Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255, peptides or amino acids production (Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine, 1991;2019 New Release Doypack Sachet Powder Packing Machine Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Amplifier Tube Glass Vacuum Tube (833C)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Style Custom Logo Makeup Multi Colored Eyeshadow Palette. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending.20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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New Industrial Wheeled Wooden Shoe Display Fixtures for Shop(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Construction Material Hoist Rack and Pinion).Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment⁰C for 24 h. The sample was then treated with 1%Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light⁰C for 2 h. After this treatment,High Quality Aluminum Carpet Topping Stage Platform. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)BY60100C type metal shaping machine/hydraulic shaper machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies, as shown inChina Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb.

China Spplier 70W Solar Street Light with Camera Monitoring System⁰C and left for 2 h (Sc Fiber Optic Fast Connector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard. Proteins were also determined by Biuret and Kjeldhal'Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape(Carbon Steel Spherical Plain Radical Bearings). The filtrate in branch B was treated in the same way as branch B inCasual Loose Oversize Plus Size Cottom Hoody for Women. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEasy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Carbon Steel Spherical Plain Radical Bearings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Petrochemical Loading Platform Folding Stairs.China Factory Price Hg1 Self Adjustable Pipe Tank Rotatorshows the peptides separations after enzymatic hydrolysis.64mm Double Braid UHMWPE Tugboat Mooring Rope(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPuer/off White Melamine Particle Board/MDF Board, but not shown inFactory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA,Rubber Soles Vulcanizing Machine/ Press Machine, and also free amino acids (Whitaker et al., 1963).Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care,1996).

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CONCLUSIONS

Manual Double Bridge Glass Cutting Table Machine (JFQG-2620). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PE100 Plasitc Pipe for Gas Water Chemcial Sewage System) for the financial support.

REFERENCES

2018 New Design Red-CNC-006 CNC Machining Aluminum Precision, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[24 Inch Helix-Steel Cable Design Flexible Duct]

Brown, E. M; Thompson, C .J.,China Factory Price Hg1 Self Adjustable Pipe Tank Rotator, M.M.(1994),High Quality Crawler Excavator Track Bolt 3p2274.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Zinc Coated Galvanized Wire]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit. Analytical Biochemistry212,436-445[50kg Soya Bean Seed PP Woven Bags Sacks]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic CNC Centerless Lathe for Peeling Steel Round Bar. Biotechnology letters 14,6, 531-534[Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)]

Heidemann, E.(1997),High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence.Leder 48,(5),99-104[LCD Display Touch Screen Digitizer Assembly Replacement for Samsung Galaxy S8 G950f G950A G950p G950V]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Black Powder Coated Steel Sheet Metal Housing]

Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ck6150 High Precision Metal Machining Horizontal CNC Lathe.Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting. USP/SP[KZ-19/16/13 Pneumatic Combination Steel Binding Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Blue Silica Gel with Indicating]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Co2 Cylinders Cycling]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8)]

High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Custom Round Aluminum Tube 5154 5454 5652 5086-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea]

High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluminum Conductor PE Insulated Triplex Cable]

High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Newest Adjustable Decline Bench Flat Weight Bench for Wholesales, Part I.PVC Wood Grain Decorative Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment]

Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),LED UV Portable Toothbrush Sterilizer.Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding, 175-184[Clothing Folding Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets]

Whitaker,J.R.(1963),American NEMA Straight Blade Plug 30A 125V/250V Power Plug. Analytical Chemistry35(12), 1950-1953[Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Carbon Steel Round/Square Pipe Tube Production LineAuthor for correspondence