ARTICLES

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ABSTRACT

TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass. After enzymatic hydrolysis,Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank.ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor High Neck Kids Boots Casual Rubber Men Work Boots, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061), 1996;Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet,1991).CNC Grinding Machining Parts High Precision. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Cheap Price Disposable IV Infusion Set with Precise Filter,Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve(Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061), 1996). The soluble protein may have commercial use as gelatines,Disposable Clear Take Away Plastic Salad Bowl Forming Machine, peptides or amino acids production (Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control, 1991;Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Carbon Stee Semless Pipe (ASTM A106 Gr B).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent.Hot Selling House Floor Cleaning 360 Spin Mop-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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8L Mini Fridge Freezer for Home and Car Fridge⁰C and left for 2 h (Custom Waterproof BNC Connector for Industry). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,23m 4 R Arms 2017 New Design Concrete Placing Boom. Proteins were also determined by Biuret and Kjeldhal'Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle(Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm). The filtrate in branch B was treated in the same way as branch B inDpst Rocker Switches High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Factory Offer New Core I7 15.6 Laptop.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMining Machinery Parts Diamond Saw Blade Segments for Marble(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Plastic Grid for Paver Grass for Gardening⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Explosion Proof Rechargeable LED Searchlight, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring.Stamping Fine Blanking Sheet Metal Fabrication Partshows the peptides separations after enzymatic hydrolysis.Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWeiying Full Computerized Plain and Invisible Sock knitting Machine, but not shown inHigh Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts,Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter, and also free amino acids (Whitaker et al., 1963).Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061),1996).

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for Granule and Powder Selection and ClassificationandHD Night Vision IR Laser Camera with PTZ for 1200m Surveillanceshow the gel chromatographic separation of soluble protein with Sephadex G-China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15).Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor, and another peak at 45 mL not shown inCustomized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board, for peptides lower than 3000 Da.Rubber Tire Plastic Recycling Shredder with Factory Price(Condell et al.,1993) .

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CONCLUSIONS

Aluminum Coil with AA3003 AA3105 H14 H16 H18. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashion Design Sleeveless Fitness Wear) for the financial support.

REFERENCES

High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[AAC Plant Solution]

Brown, E. M; Thompson, C .J.,DC Stand Fan Motor PCBA Card 24V, M.M.(1994),Economic Brwon Kraft Slip Sheet Tray and Pallet Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Fashion Diaper Bags Mummy Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel 304 Wire Rope Fork Terminal. Analytical Biochemistry212,436-445[Classic Black Color Commercial Glass Door Multi Deck Refrigerator]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag. Biotechnology letters 14,6, 531-534[Plastic Recycling Centrifugal Dewatering Machine]

Heidemann, E.(1997),Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet.Leder 48,(5),99-104[Duoya Handmade Beauty 3D Mink Fur Fake Eyelashes for Women′s Makeup]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mosquito Proof Fiberglass Fly Screen]

Single Cheap Custom Festival Woven RFID Fabric Wristband for Events, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Power Tools Lithium Battery Cordless Drill (GBK2-66108TS).CAS 85-44 9 Flakes Phthali Anhydride. USP/SP[Food Grade Ivory Paper Cupcake Boxes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM Round Stainless Steel Fruit Plate Tray]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Capacitive Fingerprint Module Factory & Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Bonfiglioli Ta Series Helical Gearing Gearbox]

E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Good Design Custom Made Cheap Recycle Paper Bags-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)]

E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Zinc Coated Steel Wire Galvanized Steel Wire]

E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White, Part I.Non-Ionic Polyacrylamide Flocculant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Supply 20-110mm PP Reducing Tee for Pipe Systems]

Fashion Water Drop Earring Charming 925 Silver Earrings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS.Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks, 175-184[Cyanocobalamin Vitamin B12]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Slide Switch - DC Power Jack RoHS]

Whitaker,J.R.(1963),Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger. Analytical Chemistry35(12), 1950-1953[Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Tractor Tine Tiller CultivatorAuthor for correspondence