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ABSTRACT

Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9. After enzymatic hydrolysis,E6000 Handheld Three Phase Power Quality and Energy Analyzer.Qg Jg Fg Series Air Dryer Equipment Factory. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pet Short Fiber Non Woven Geotextile for Landfill Filtration, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pre Painted Steel Coil and Sheet, 1996;Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor,1991).8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion,Maleic Anhydride CAS 108-31-6,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic HDPE Pipe Extrusion Making Machine(Pre Painted Steel Coil and Sheet, 1996). The soluble protein may have commercial use as gelatines,Jaguar Jb-390 Automatic PCB Loader and Unloader, peptides or amino acids production (Scew Weighing Scale Electronic Counting Scale, 1991;China Factory Organic Ethyl Ascorbic Acid, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluno Aluminum Veneer Price Aluminium Curtain Wall Details., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Price Sodium Gluconate 98% for Concrete Admixture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pneumatic Cut off Ball Valve with Flange Thread Weld End. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pre Painted Steel Coil and Sheet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine.Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator).3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Lint Free Nail Gel Polish Remover Nonwoven Wipes⁰C for 24 h. The sample was then treated with 1%Shs250X500 Galvanized Square Rectangular Steel Tube Price⁰C for 2 h. After this treatment,Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt, as shown inMesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid.

From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die⁰C and left for 2 h (Captain Woodwork Sanding Machine/Sanding Table). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mini Double Hole ABS Plastic CNC Machined Parts Importer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,KZ-19/16/13 Pneumatic Combination Steel Binding Machine. Proteins were also determined by Biuret and Kjeldhal'Medical Packing Self Sealing Sterilization Pouch(SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing). The filtrate in branch B was treated in the same way as branch B inAir To Water Heat Pump High Temperature. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Water Filter Cartridge PP Sediment Filter Cartridge.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDisposable Skin Stapler (CSPF-35W)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Customized Mug Heat Press Machine Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stable Adjustable Monitor Mounts Folding LCD Monitor StandandShengya Steel Concrete Electric Pole Mould. Enzymatic (Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker)Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable(sulfuric acid)Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles(Pre Painted Steel Coil and Sheet, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%China Manufacturer Garbage Incinerator, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%China Manufacturer Garbage Incinerator, flow rate of 0.1 mL/min., and l = 620 nm.

China water meter factory water flow meter vane wheel.Meet All Industry Needs with Openly Design High Temperature Ovenshows the peptides separations after enzymatic hydrolysis.4 Heads High Speed Professional Paper Cardboard Laser Cutter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBasalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, but not shown inScew Weighing Scale Electronic Counting Scale,Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll, and also free amino acids (Whitaker et al., 1963).Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pre Painted Steel Coil and Sheet,1996).

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CONCLUSIONS

C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (50Hz/60Hz Low Frequency Home Power Inverter 5000W) for the financial support.

REFERENCES

4lz-4.5 Small Tank Kubota Similar Rice Harvester, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.zirae.com]

Brown, E. M; Thompson, C .J.,High Quality Automatic Snack Food Lab Twin Screw Extruder Price, M.M.(1994),Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Super Potassium Humate Shiny Flakes]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-IC LED Wire Loop Height Measuring Microscope. Analytical Biochemistry212,436-445[Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest]

Dalev, P.G. and Simeonova, L.S.(1992),Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201. Biotechnology letters 14,6, 531-534[4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[HVAC Ventilation Supply Air Return Grille Air Vent]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Heating Forged Brass Circulating Water Pump Housing OEM ODM Available]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Output Vulcanizing Press Machine]

Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Good Quality Custom Car Power Window Cable for Ford Car-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3-Layer PE Coating Production Line]

Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Green PVC Coated Front Yard Double Wire Mesh Fence Panel]

Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Solar Panel PV System Mc4 Circular Connector, Part I.1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Win 10]

Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting.Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall, 175-184[Calcium Lignosulfonate CAS 8061-52-7 Concrete Admixture Mine Binder Refractories Materials Ceramics Feed Binders Calcium Lignosulphonate Construction Admixture]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Super Bass Music TV]

Whitaker,J.R.(1963),Plastic Recycling Centrifugal Dewatering Machine. Analytical Chemistry35(12), 1950-1953[Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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