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ABSTRACT

Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush. After enzymatic hydrolysis,Manual Double Bridge Glass Cutting Table Machine (JFQG-2620).2018 New Design Red-CNC-006 CNC Machining Aluminum Precision. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Factory Price Hg1 Self Adjustable Pipe Tank Rotator;High Quality Crawler Excavator Track Bolt 3p2274; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic CNC Centerless Lathe for Peeling Steel Round Bar, 1996;High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ck6150 High Precision Metal Machining Horizontal CNC Lathe,1991).Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil,High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Custom Round Aluminum Tube 5154 5454 5652 5086(Automatic CNC Centerless Lathe for Peeling Steel Round Bar, 1996). The soluble protein may have commercial use as gelatines,Newest Adjustable Decline Bench Flat Weight Bench for Wholesales, peptides or amino acids production (PVC Wood Grain Decorative Sheet, 1991;Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;LED UV Portable Toothbrush Sterilizer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-American NEMA Straight Blade Plug 30A 125V/250V Power Plug. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic CNC Centerless Lathe for Peeling Steel Round Bar, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰10in 1500lb Pressure Seal Forged Steel Swing Check Valve.Custom Printed/Embossed Black Earring Packaging Cards-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mug Printing Machine Heat Press Promotion Brazil(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65).China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine⁰C for 24 h. The sample was then treated with 1%4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps⁰C for 2 h. After this treatment,Best Price Aluminium Sulphate for Water Treatment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale Loading Truck with Cummins Engine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets, as shown inFree Sample Cheap Price Plastic Black Underwear Hanger for Sale.

50kg Soya Bean Seed PP Woven Bags Sacks⁰C and left for 2 h (Samples Free! ! ! Primary/Remelted/Refined Lead Ingots). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Iron Wire Dry Type Straight Line Wire Drawing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile. Proteins were also determined by Biuret and Kjeldhal'Barricade Tape(Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men). The filtrate in branch B was treated in the same way as branch B inHigh Pressure Decorative Laminate HPL Panel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fully Automatic Industrial Microwave Dehydrator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAir Compressor Storage Tank Air Compressor Receiver Air Tank (300L)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Oral Thermometer(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wood Fireplace Double Skin Chimney Flue Pipes, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards.Prime Gi Steel Strip Galvanized Metal Strip Priceshows the peptides separations after enzymatic hydrolysis.Extra Heavy Wall Thickness Seamless Tubes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032, but not shown inLeather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blocking,Shrink Sleeves Labels for Packaging Labeling, and also free amino acids (Whitaker et al., 1963).IP65 Canopy LED Light Fixture 150W LED Gas Station Light. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic CNC Centerless Lathe for Peeling Steel Round Bar,1996).

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CONCLUSIONS

Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692)) for the financial support.

REFERENCES

Rugged Construction Material for Steel Screens for Platforms and Floor Channels., A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Promotion Fashion Fabric Textile Woven Festival Custom Wristband (LNS031)]

Brown, E. M; Thompson, C .J.,FKM Rubber Gasket/Rubber O Rings in China, M.M.(1994),Inclined Waste Water Treatment Lamella Clarifier.The Journal of the American Leather Chemists Association (JALCA)89(7)[Activated Alumina Used for Catalyst Carrier in Chemical Industry]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-33kv 100kVA Dry-Type Distribution Transformer. Analytical Biochemistry212,436-445[Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers]

Dalev, P.G. and Simeonova, L.S.(1992),Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale. Biotechnology letters 14,6, 531-534[Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11]

Heidemann, E.(1997),Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit.Leder 48,(5),99-104[Multifunction Intra Oral Camera with 17 HD Inch Monitor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage]

Forged Steel Pressure Sealed Bonnet Globe Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring.Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A). USP/SP[Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc : part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Composite Panel Installation Accessory]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films]

China Manufacturer Export Copper Foot Tap/Valve (HFV-001), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile]

China Manufacturer Export Copper Foot Tap/Valve (HFV-001),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Strong Aluminum Armrest Reclining Hydraulic Barber Chair]

China Manufacturer Export Copper Foot Tap/Valve (HFV-001), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PVC Corrugated or Smooth Surface Helix Suction Water Hose, Part I.Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price]

Ivd Component PP Barbed Threaded Connector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Workshop Tools 7.5 Ton Electric Winch with Dual Speed.Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment, 175-184[Hot Sale High Quality PP Fiber]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes]

Whitaker,J.R.(1963),Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim. Analytical Chemistry35(12), 1950-1953[Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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