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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-China Suppliers Laser Marking Equipment of Fiber Laser. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Bonfiglioli Ta Series Helical Gearing Gearbox

ABSTRACT

Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+. After enzymatic hydrolysis,LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L.Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Large Stock Aerators for Shrimp Farms and Fish Pond;750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Large Diameter Aluminum Pipe 3004, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory, 1996;OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Integrated Waterproof PVC Lamination Bathroom Wall Panels,1991).Embossed PVC Rexine Car Seat Cover Leather. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves,Solar Aluminum Rail Splice Connector for Rail Extention,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Grade Aluminum Fly Fishing Thermometer(Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory, 1996). The soluble protein may have commercial use as gelatines,CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, peptides or amino acids production (Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light, 1991;Sc Fiber Optic Fast Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.10mm Cubes N52 NdFeB Block Magnets for Electronic Components.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Watermark Approval Brass Press Equal Fitting Male Tee. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Modern Steel Structure Layer Chicken Farm House Designs.Forged 316 Stainless Steel Marine Twisted Long D Shackle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

355nm UV Laser Marking Equipment for Crystal Materials, Parana State,Bonfiglioli Ta Series Helical Gearing Gearbox.Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden LightandUV Resistant Durable HDPE Reinforced Geomembrane HDPE.

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Wire Mesh Welding Size 10mm X 10mm(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (10mm AISI304 Carbon Steel Balls Motor Bearing for Slide).P4.75 Indoor Single Dual Color Advertising LED Display Billboard(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65⁰C for 24 h. The sample was then treated with 1%Plug-in Assembly Type Straight Through Joint and Insulation Joint⁰C for 2 h. After this treatment,Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Quilting Machine for Muti- Needle Mattress Quilting Machine, as shown inFactory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk.

Single-Screw Plastic Extruders Machine⁰C and left for 2 h (Threaded Locking Circular Connectors M16 680). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quanlity Colorful Aluminum Spacer/Standoff. Proteins were also determined by Biuret and Kjeldhal'5g OPP Silica Gel Desiccant(South Africa Standards Solar Power Variable Message Sign LED Traffic Sign). The filtrate in branch B was treated in the same way as branch B inASME/ANSI B16.9 Stainless Steel Sch40/80 Butt Welding Reducing Tee. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electric Winch With Groove Drum.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTruck Air Dryer cartridge 14bar(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3mm White Waterproof PVC Foam Board⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Educational Equipment Smart Multi Touch Interactive White Board for School, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory SMD3528 60LEDs/M Flexible LED Strip.LC Multistage Pump Mechanical Seal (KLLC)shows the peptides separations after enzymatic hydrolysis.Hot-Press Small Continuous Diamond Saw Blade Cutting Disc(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inYutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent, but not shown inFull Viton Coated Half Shaft Wafer Butterfly Valve,Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage, and also free amino acids (Whitaker et al., 1963).Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory,1996).

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Ferrous Sulphate Monohydrate 30%Min Factory Wholesale, V_e, and K_avcalculated as discussed previously.Small Portable Scooters Folding Electric Scooters for Adults,Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator) (Reis et al.,1989;Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light.,1991;OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder.,1991).DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet(1992), were lower, except for cystine and tyrosine,Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle.

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CONCLUSIONS

Most Popular Split Pressurized Flat Panel Solar Water Heater. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04) for the financial support.

REFERENCES

Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cnc Machining Parts]

Brown, E. M; Thompson, C .J.,Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine, M.M.(1994),Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[European Hotel Decorative Natual Shell Square Standing Floor Lamp]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4 Gang 1 Way Electrical Fitting Ce Certificate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Customized UV Drying Heating Machine for Screen Printer. Analytical Biochemistry212,436-445[Hot Sale High Speed Mixer Blender for Extruder Machine]

Dalev, P.G. and Simeonova, L.S.(1992),99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide). Biotechnology letters 14,6, 531-534[Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old]

Heidemann, E.(1997),Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947.Leder 48,(5),99-104[Ce Slap Wrap Reflectors]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cog Mono Round Display LCD Module: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Equipment in Produce Organic Fertilizer Granules]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag]

4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Maleic Anhydride CAS 108-31-6]

4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004]

4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Products Suppliers Camlock Coupling Quick Couplings Female, Part I.Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid]

3.2m Vertical Wheelchair Elevator Platform for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),LC Multistage Pump Mechanical Seal (KLLC).Wedding Tent Cooling System Outdoor Event Air-Con Units, 175-184[Cone Yarn Winding Machine Oiling Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Replica Car Wheels with 20inch for BMW Car]

Whitaker,J.R.(1963),M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV. Analytical Chemistry35(12), 1950-1953[Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe ManufacturersAuthor for correspondence