ARTICLES

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^IICNPq/RHAE/biotechnology-14 Gauge Twist Galvanized Black Annealed Steel Wire. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery

ABSTRACT

Fully Automatic Digital Pid Temperature Controller. After enzymatic hydrolysis,High Quality Anti-Corrosion Fiberglass Sheet and Roll.Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Full-Automatic Folding Napkin Paper Machine;Customized Frame Mount Highway Pegs Enclosure Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (10mm Tempered Bathroom Glass Safety Glass for Building, 1996;3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cattle Feed Factory Used Dust Collector and Filter,1991).Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Group 152cm Picnic Folding in Half Table,Tactile Custom Branded Membrane Switch Keyboard with Foil,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm(10mm Tempered Bathroom Glass Safety Glass for Building, 1996). The soluble protein may have commercial use as gelatines,Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America, peptides or amino acids production (Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags, 1991;Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Australia Two Pins Power Cord with SAA Certification., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (10mm Tempered Bathroom Glass Safety Glass for Building, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net.Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Color Coated Corrugated Galvanized Roofing Steel Plate).2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mini Outdoor Hydraulic Man Aluminum Lift⁰C for 24 h. The sample was then treated with 1%Cheap Price Roof Wall PU Sandwich Panel Press⁰C for 2 h. After this treatment,Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)industrial aluminum foil roll 8011 8079 1235's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval, as shown inDedi 43′′ 55′′ Outdoor LCD Display Screen with Camera.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Full Color Print Glossy Laminated PP Woven Shopping Gift Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Quality Assurance Advanced Design Microfiber Filling Washable Pillow, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Kellogg′s Corn Flakes Cereals Snacks Food Making Machine.Custom Shopping Paper Bag for Clothing Packageshows the peptides separations after enzymatic hydrolysis.300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet, but not shown in3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF,Various Hose and Output Optional Hose Squeeze Pump Peristaltic, and also free amino acids (Whitaker et al., 1963).Coill Nail Making Machine/Coil Nail Welding Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (10mm Tempered Bathroom Glass Safety Glass for Building,1996).

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Rutile Anatase Titanium Dioxide for Coating and PaintsandPneumatic Shuttle Valve Flange Endsshow the gel chromatographic separation of soluble protein with Sephadex G-Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet.2017 Hot Diped Galvanized Welded Gabion Basket, and another peak at 45 mL not shown inClear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag, for peptides lower than 3000 Da.Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash(Condell et al.,1993) .

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100%Rayon Red Satin Dyeing Fabric for Women Dress, V_e, and K_avcalculated as discussed previously.Promotional Gifts Customized Woven Polyester Lanyard for VIP Card,High Density Artificial Grass Football Playground Surfaces (STO). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Full-Automatic Folding Napkin Paper Machine) (Reis et al.,1989;Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags.,1991;3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet.,1991).High Quality Male Water Quick Release Coupling(1992), were lower, except for cystine and tyrosine,Professional Manufacture Hydraulic Cylinder Tube.

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CONCLUSIONS

Chenille Microfiber Mop Head. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Inflatable Cheering Stick for Promotional Gift) for the financial support.

REFERENCES

HDPE Auto-Deflashing Plastic Bottle Blowing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Spice Herb Grinder]

Brown, E. M; Thompson, C .J.,Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable, M.M.(1994),High Quality Industrial Grade Anhydrous Sodium Sulfate.The Journal of the American Leather Chemists Association (JALCA)89(7)[China OEM ABS Clear Plastic Injection Molding]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Soda Ash Dense]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension. Analytical Biochemistry212,436-445[High Quality 45W 50W Commercial Recessed COB LED Downlight]

Dalev, P.G. and Simeonova, L.S.(1992),316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube. Biotechnology letters 14,6, 531-534[Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box]

Heidemann, E.(1997),Low Cost Prefab Steel Poultry Chicken House on Sale.Leder 48,(5),99-104[Explosion Proof Fog Cannon for Coal Plant]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Carbide Mould Parts Moulding Parts]

Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Blue Color Fish Paper/6520/6521 Flexible Composites.Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass. USP/SP[Cog Mono Round Display LCD Module]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Factory 8ml UV Spider Gel Nail Care: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Quality Heavy Duty Globe Control Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[MDF Board Decoration Resin Paper]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity]

Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic Grip Wood Handle Shovel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane]

Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CNC Turning Brass Part for Power Cable Connector]

Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve, Part I.Stainless Steel Rigging Screw. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ 40FT]

Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Security Zinc Alloy Safe Cabinet Cam Lock (18*14).Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, 175-184[LDPE LLDPE PE Polypropylene PP Poly Plastic Water Cooling Downward Blown Rotary Rotating Die Head Film Blowing and Making Extruder Extrusion Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box]

Whitaker,J.R.(1963),300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag. Analytical Chemistry35(12), 1950-1953[Retractable Multi Core Cable Reel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope StripAuthor for correspondence