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ABSTRACT

OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack. After enzymatic hydrolysis,Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper.Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Anti-Pollution Type 160kn Insulator Porclelain Disc, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Nickel 200 Floating Ball Valve, 1996;3-Layer PE Coating Production Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase,1991).Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed,Wooden Queen/King Size Japan Sofa Bed G1162,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,J55 Short Round Thread Seamless Casing Pipe(Nickel 200 Floating Ball Valve, 1996). The soluble protein may have commercial use as gelatines,2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine, peptides or amino acids production (3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube, 1991;Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dust Hood Fume Extraction Systems for Robotic Laser Cutting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Nickel 200 Floating Ball Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector.Nordic Solid Wood Dining Chair/Smile Chair-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine, Parana State,Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB.IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Nickel 200 Floating Ball Valve, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the45ton Proof Load Testing Water Bag for Life BoatandChubby 163cm Fat Big Ass Female Real Sex Doll for Man.

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Tent Windows Use China Made PVC Transparent Clear Mesh Tarp(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar).13 Oz Flex Banner PVC Frontlit Banner Material(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller⁰C for 24 h. The sample was then treated with 1%3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables⁰C for 2 h. After this treatment,Chubby 163cm Fat Big Ass Female Real Sex Doll for Man. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)10mm AISI304 Carbon Steel Balls Motor Bearing for Slide, as shown inOEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale.

Different Size of Ring NdFeB Magnet⁰C and left for 2 h (High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Investment Precision Casting for Valve Body with Machining. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Unique Best Quality Copper Home Alcohol Distiller for Sale. Proteins were also determined by Biuret and Kjeldhal'Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories(Decorative Aluminum Wall Cladding Panel with Durable Painting Finish). The filtrate in branch B was treated in the same way as branch B inFabric Auditorium Seat Home Theater Chair (M2). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Drive Travel Car Inflatable Bed. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.TPU TPR Rubber Band Plastic Extruder Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wheel Loader Transmission Gear Box/Customized Casting Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

China Manufacturer Export Copper Foot Tap/Valve (HFV-001)andAuto Spare Parts Ignition Coil 22448-2y001. Enzymatic (3D Good Quality Laser Engraving Machine for Souvenirs Shop)Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories(sulfuric acid)3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording(Nickel 200 Floating Ball Valve, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Reverse Flow Configuration Axial Fan, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Reverse Flow Configuration Axial Fan, flow rate of 0.1 mL/min., and l = 620 nm.

Handcraft Wood Jewelry Storage Box with Lock and Key.Smart Big Hydroponic Indoor Home Garden Plantershows the peptides separations after enzymatic hydrolysis.TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, but not shown in Fabric,Single Spindle Micro CNC Lathe Ck0640, and also free amino acids (Whitaker et al., 1963).Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Nickel 200 Floating Ball Valve,1996).

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Nitinol Disposable Guide Wire, V_e, and K_avcalculated as discussed previously.Breathable Surgical Paper Tape/ Micropore Medical Tape,Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (12oz 16oz Glass Mason Jar with Handle and Straw) (Reis et al.,1989;3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube.,1991;3-Layer PE Coating Production Line.,1991).Crane Hook Qtz250 (7032) with 70m Jib Length(1992), were lower, except for cystine and tyrosine,China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System.

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CONCLUSIONS

Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display) for the financial support.

REFERENCES

Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Common Nails On Sale]

Brown, E. M; Thompson, C .J.,Online High Sensitivity Checkweigher with Pusher Bar, M.M.(1994),Piggery Farm Pig/Sow Gestation Equipment From China Factory.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Underground Carport]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Lw250*700 Spiral Discharge Decanter Separator for Water Treatment. Analytical Biochemistry212,436-445[Commecial Use Watch Display Cabinet for Watch Store Interior Design]

Dalev, P.G. and Simeonova, L.S.(1992),Single Cheap Custom Festival Woven RFID Fabric Wristband for Events. Biotechnology letters 14,6, 531-534[Ce Marked Medical Syringe Pump (WP08N)]

Heidemann, E.(1997),Children Gift Outdoor Wooden Rocking Horse Toy.Leder 48,(5),99-104[Popular Pattern Flower Embroidered Mesh Tulle Lace Fabric for Dress]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD]

China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330.TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass. USP/SP[China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminum Sheet Metal Case Parts for Industrial Computer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Big Discount Surface Pro 1 Pro 2 Rt Charger - 24V 800W Modified Sine Wave Inverter - Ligao]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wall Mounting Metal Control Box Control Desk]

ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine]

ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets]

ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe, Part I.Outdoor High Neck Kids Boots Casual Rubber Men Work Boots. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S)]

Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector.2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet, 175-184[m.pushpullconnector.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Etched Stainless Steel Titanium Sheet]

Whitaker,J.R.(1963),CNC Grinding Machining Parts High Precision. Analytical Chemistry35(12), 1950-1953[CNC Turned Aluminum Anodized Auto Part]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ml33-2mm Eddy Current Linear Position/Displacement SensorAuthor for correspondence