ARTICLES

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^IICNPq/RHAE/biotechnology-Ml33-2mm Eddy Current Linear Position/Displacement Sensor. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat

ABSTRACT

Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain. After enzymatic hydrolysis,Wholesale Long Lasting Custom Private Label Glitter Lip Gloss.ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Dyed Colored Sand, Natural Coloured Sand, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps, 1996;Various Bridge NdFeB Motor Magnets with High Performance., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (White Melamine Powder for Industrial Uses,1991).UV Resistant Durable HDPE Reinforced Geomembrane HDPE. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2070 4th Axis CNC, Atc CNC Router Machine, Carving Engraving Machinery with High Z Axis,Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PE Film Blowing Machine (SJ-1000-1300-1500)(China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps, 1996). The soluble protein may have commercial use as gelatines,Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag, peptides or amino acids production (304 304L 904L 309S 310S Stainless Steel Design Checkered Plate, 1991;High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2017 Factory Directly Oversized Women Sunglasses., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quanlity PTFE Thread Seal Tape.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fiberglass Grating, FRP Grating, GRP Grating. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium.Rutile Titanium Dioxide Exterior Emulsion Paint-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF, Parana State,Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat.Magnetic Latching Relay for Health Life, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePet Strapping Band Production Lineand500ml PP Sport Water Bottle with Ball.

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More Cavities Plastic Mould Design(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple).Energy Conservation Electric Single Servo Turret Punch Press Machine D-Es300(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality 14AWG 16AWG USA spiral Power Cord to IEC C13⁰C for 24 h. The sample was then treated with 1%Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex⁰C for 2 h. After this treatment,Soft magnetic alloy 1J38 rod / Ni38Cr13/38H14X. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Current Temperature Rising Test Large Current Temperature Rising Test System's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Animal Manure Fertilizer Fermentation Machine, as shown inIndoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDamage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Men Casual Short Pants Custom Quality Running Sports Wear Shorts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Horizontal Window Shade Blind Zebra Dual Roller Blinds Day, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wire Mesh Welding Size 10mm X 10mmandNew Fashion Diaper Bags Mummy Bag. Enzymatic (Mellow OEM Plastic Pet House Ml-HP004)12/24V 150ah Power Battery Charger(sulfuric acid)1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable)(China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Air Duct Making Machine, HVAC Square Duct Making Machine for Sale, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Air Duct Making Machine, HVAC Square Duct Making Machine for Sale, flow rate of 0.1 mL/min., and l = 620 nm.

China Brass Precision CNC Machining Spare Auto Parts with Competitive Price.Promotional Gifts Customized Woven Polyester Lanyard for VIP Cardshows the peptides separations after enzymatic hydrolysis.Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPA Flexible Plastic Corrugated Loom Tubing, but not shown in1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll,044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head, and also free amino acids (Whitaker et al., 1963).Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps,1996).

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Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8, V_e, and K_avcalculated as discussed previously.Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home,AKD Wax 1895/Alkyl Ketene Dimer 1895. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb) (Reis et al.,1989;304 304L 904L 309S 310S Stainless Steel Design Checkered Plate.,1991;Various Bridge NdFeB Motor Magnets with High Performance.,1991).22.8V 150W Medical Halogen Lamp(1992), were lower, except for cystine and tyrosine,Aluminum Conductor PE Insulated Triplex Cable.

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CONCLUSIONS

Home Appliance Blender Juicer Motor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube) for the financial support.

REFERENCES

Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Neodymium Magnets]

Brown, E. M; Thompson, C .J.,Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices, M.M.(1994),13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Carbon Steel 20g Gr. B Steam Cooling Fin Tube]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Factory Price Hg1 Self Adjustable Pipe Tank Rotator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price. Analytical Biochemistry212,436-445[Puer/off White Melamine Particle Board/MDF Board]

Dalev, P.G. and Simeonova, L.S.(1992),Tractor Tine Tiller Cultivator. Biotechnology letters 14,6, 531-534[Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA]

Heidemann, E.(1997),Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins.Leder 48,(5),99-104[250ml Glass Dairy Milk Bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pickled Cucumber Production Line/Making Machine]

Pipe Connection Types of Pressing Scaffolding Universal Clamp, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle.Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics. USP/SP[Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Factory 8ml UV Spider Gel Nail Care]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-LSR Injection Mold Tooling for Medical Nipple: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Home Appliance Blender Juicer Motor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Recycled Paper Pulp Wholesale Egg Carton Egg Tray for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic]

Nail Stamping Plates Custom Metal Part Sheet Metal Stamping, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Sports Jacket Men Outdoor Waterproof Jackets-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hotel Security Improvement Zinc Alloy Door Chain]

Nail Stamping Plates Custom Metal Part Sheet Metal Stamping,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[33kv 100kVA Dry-Type Distribution Transformer]

Nail Stamping Plates Custom Metal Part Sheet Metal Stamping, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller, Part I.Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ GRP Grating]

22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Small Steel Silo for Sale/Steel Hopper.Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, 175-184[Artificial Grass For Sports Flooring High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle]

Whitaker,J.R.(1963),Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights. Analytical Chemistry35(12), 1950-1953[High Zinc Coated Galvanized Wire]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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