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ABSTRACT

10mm AISI304 Carbon Steel Balls Motor Bearing for Slide. After enzymatic hydrolysis,Different Size of Ring NdFeB Magnet.Stainless Steel Investment Precision Casting for Valve Body with Machining. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Drive Travel Car Inflatable Bed, 1996;Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China PCBA Supplier Custom Game Controller PCB,1991).TPU TPR Rubber Band Plastic Extruder Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wheel Loader Transmission Gear Box/Customized Casting Parts,SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Auto Spare Parts Ignition Coil 22448-2y001(Drive Travel Car Inflatable Bed, 1996). The soluble protein may have commercial use as gelatines,3D Good Quality Laser Engraving Machine for Souvenirs Shop, peptides or amino acids production (Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories, 1991;3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Reverse Flow Configuration Axial Fan., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Handcraft Wood Jewelry Storage Box with Lock and Key.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Drive Travel Car Inflatable Bed, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Single Spindle Micro CNC Lathe Ck0640.Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pipe Thermostat with Sensor Ce Certificate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Customized Mug Heat Press Machine Price).Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar⁰C for 24 h. The sample was then treated with 1%Online High Sensitivity Checkweigher with Pusher Bar⁰C for 2 h. After this treatment,Piggery Farm Pig/Sow Gestation Equipment From China Factory. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Lw250*700 Spiral Discharge Decanter Separator for Water Treatment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Single Cheap Custom Festival Woven RFID Fabric Wristband for Events, as shown in5g OPP Silica Gel Desiccant.

Children Gift Outdoor Wooden Rocking Horse Toy⁰C and left for 2 h (Ivd Component PP Barbed Threaded Connector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass. Proteins were also determined by Biuret and Kjeldhal'Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank(Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine). The filtrate in branch B was treated in the same way as branch B in200ml Empty Colorful Empty Plastic Pet Mist Spray Bottle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avXCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control.Factory Custom Round Aluminum Tube 5154 5454 5652 5086shows the peptides separations after enzymatic hydrolysis.Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS), but not shown inWholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper,Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge, and also free amino acids (Whitaker et al., 1963).Carbon Stee Semless Pipe (ASTM A106 Gr B). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Drive Travel Car Inflatable Bed,1996).

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CONCLUSIONS

Hsc Heavy Duty 5 Ton Chain Pulley Block. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Nitinol Disposable Guide Wire) for the financial support.

REFERENCES

Outdoor Christmas Decoration Highway Street Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Abrasion Resistance PVC Man-Made Leather for Car Upholstery]

Brown, E. M; Thompson, C .J.,Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length, M.M.(1994),China Cheap Newest 70*77cm XPE Foam 3D Wallpaper.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rubber Tire Plastic Recycling Shredder with Factory Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-48V 200ah UPS Battery Backup for Telecom. Analytical Biochemistry212,436-445[OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray. Biotechnology letters 14,6, 531-534[High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Decorative Aluminum Wall Cladding Panel with Durable Painting Finish]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Smart Big Hydroponic Indoor Home Garden Planter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-8L Mini Fridge Freezer for Home and Car Fridge: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electric Pedal Assist Bicycle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Fabric]

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Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.]

Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle, Part I.China Factory Offer New Core I7 15.6 Laptop. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Nice Quality Coloring Printing Children Board Book Printing]

Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mining Machinery Parts Diamond Saw Blade Segments for Marble.Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding, 175-184[Blood Oxygen Monitor Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling]

Whitaker,J.R.(1963),China Plastic Grid for Paver Grass for Gardening. Analytical Chemistry35(12), 1950-1953[Mini Double Hole ABS Plastic CNC Machined Parts Importer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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