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ABSTRACT

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Key words;Plastic Grip Wood Handle Shovel;2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Rigging Screw, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, 1996;Security Zinc Alloy Safe Cabinet Cam Lock (18*14)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bubule 17 Inch PP Carry on Kids Travel Luggage for Children,1991).300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Farm Machinery Auto Mechanical Straw Crusher for Poultry,Gloss Art Paper Made Packing Package,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist(Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, 1996). The soluble protein may have commercial use as gelatines,External Wall Material Panels/Aluminum Composite Sheet, peptides or amino acids production (Cheap Price Paper Straws Individually Wrapped Drinking Straw, 1991;Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Medical Cable Assembly Wire Harness Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Wood Transport Log/Timber Truck Trailers for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer.China Hebei Phosphating Black Heading Machine Drywall Screw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Strong Rare Earth Permanent Wind Driven Generator Magnets(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors).100% PVC Resin PVC Clear Transparent Film for Shower Curtain(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hotel Security Improvement Zinc Alloy Door Chain⁰C for 24 h. The sample was then treated with 1%Anti-Pollution Type 160kn Insulator Porclelain Disc⁰C for 2 h. After this treatment,Nickel 200 Floating Ball Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3-Layer PE Coating Production Line's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase, as shown in14 Gauge Twist Galvanized Black Annealed Steel Wire.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dust Hood Fume Extraction Systems for Robotic Laser Cutting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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3DTALK DS200 Large Printing Size 3D Printer for Dental HygienistandPlastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machineshow the gel chromatographic separation of soluble protein with Sephadex G-13 Oz Flex Banner PVC Frontlit Banner Material.Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller, and another peak at 45 mL not shown inVenlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden, for peptides lower than 3000 Da.3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables(Condell et al.,1993) .

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CONCLUSIONS

Unique Best Quality Copper Home Alcohol Distiller for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Industry Fluorine Lined Butterfly Valve Lug Type) for the financial support.

REFERENCES

Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Small Laser Engraving]

Brown, E. M; Thompson, C .J.,Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector, M.M.(1994),Drive Travel Car Inflatable Bed.The Journal of the American Leather Chemists Association (JALCA)89(7)[4K Giant Whiteboard Interactive Panel for University Whiteboard]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mini Freeze Dryer for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors. Analytical Biochemistry212,436-445[Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle]

Dalev, P.G. and Simeonova, L.S.(1992),China PCBA Supplier Custom Game Controller PCB. Biotechnology letters 14,6, 531-534[Wholesale Long Lasting Custom Private Label Glitter Lip Gloss]

Heidemann, E.(1997),TPU TPR Rubber Band Plastic Extruder Machine.Leder 48,(5),99-104[Pin Mill]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Bending Shrapnel]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale Sports Jacket Men Outdoor Waterproof Jackets]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Auto Spare Parts Ignition Coil 22448-2y001: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Round Roof]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid]

3D Good Quality Laser Engraving Machine for Souvenirs Shop, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5 Gallon Pure Water Bottle Non-Spill Cap]

3D Good Quality Laser Engraving Machine for Souvenirs Shop,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector]

3D Good Quality Laser Engraving Machine for Souvenirs Shop, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording, Part I.Reverse Flow Configuration Axial Fan. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Price Sodium Gluconate 98% for Concrete Admixture]

Handcraft Wood Jewelry Storage Box with Lock and Key, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit.Single Spindle Micro CNC Lathe Ck0640, 175-184[Universal Couplings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter]

Whitaker,J.R.(1963),Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring. Analytical Chemistry35(12), 1950-1953[Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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