ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Quality Factory 8ml UV Spider Gel Nail Care

ABSTRACT

Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers. After enzymatic hydrolysis,Plastic Grip Wood Handle Shovel.2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Rigging Screw;Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Security Zinc Alloy Safe Cabinet Cam Lock (18*14), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, 1996;300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Farm Machinery Auto Mechanical Straw Crusher for Poultry,1991).Gloss Art Paper Made Packing Package. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist,External Wall Material Panels/Aluminum Composite Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cheap Price Paper Straws Individually Wrapped Drinking Straw(Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, 1996). The soluble protein may have commercial use as gelatines,Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml, peptides or amino acids production (Medical Cable Assembly Wire Harness Manufacturer, 1991;Hot Sale Wood Transport Log/Timber Truck Trailers for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Hebei Phosphating Black Heading Machine Drywall Screw. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Strong Rare Earth Permanent Wind Driven Generator Magnets.Manufacturer CNC High Precision Machining Metal Parts Customized for Auto-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Hotel Security Improvement Zinc Alloy Door Chain(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector).Anti-Pollution Type 160kn Insulator Porclelain Disc(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Nickel 200 Floating Ball Valve⁰C for 24 h. The sample was then treated with 1%3-Layer PE Coating Production Line⁰C for 2 h. After this treatment,Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed, as shown inFruit/ Vegetable Double Spray High Pressure Cleaner for Sale.

Wooden Queen/King Size Japan Sofa Bed G1162⁰C and left for 2 h (Plastic Grip Wood Handle Shovel). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.J55 Short Round Thread Seamless Casing Pipe. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine. Proteins were also determined by Biuret and Kjeldhal'3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube(22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine). The filtrate in branch B was treated in the same way as branch B inFast Filling Waterproof Inflatable Lazy Air Sofa Outdoor Sofa. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDeck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Nordic Solid Wood Dining Chair/Smile Chair, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factoryand1200X1000X810 Strong Collapsible Plastic Containers for Storageshow the gel chromatographic separation of soluble protein with Sephadex G-3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables.Chubby 163cm Fat Big Ass Female Real Sex Doll for Man, and another peak at 45 mL not shown in2019 New Advertising Inflatable LED Jellyfish Balloon for Sale, for peptides lower than 3000 Da.Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V(Condell et al.,1993) .

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CONCLUSIONS

Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack) for the financial support.

REFERENCES

Drive Travel Car Inflatable Bed, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Aoi Cost]

Brown, E. M; Thompson, C .J.,Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors, M.M.(1994),China PCBA Supplier Custom Game Controller PCB.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Factory Supply Stainless Steel T Shape Bolt]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-TPU TPR Rubber Band Plastic Extruder Machine. Analytical Biochemistry212,436-445[Creative New Product Coin Holder Keychain]

Dalev, P.G. and Simeonova, L.S.(1992),Wheel Loader Transmission Gear Box/Customized Casting Parts. Biotechnology letters 14,6, 531-534[Hsc Heavy Duty 5 Ton Chain Pulley Block]

Heidemann, E.(1997),SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH).Leder 48,(5),99-104[Rtzf-D Auto Fast Food Take Away Lunch Box Making Forming Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[14 Heads Double Door Multihead Weigher]

Blue Color Fish Paper/6520/6521 Flexible Composites, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Auto Spare Parts Ignition Coil 22448-2y001.3D Good Quality Laser Engraving Machine for Souvenirs Shop. USP/SP[Glass Water Jet Cutting Machine (Skwj-4018A)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic CNC Centerless Lathe for Peeling Steel Round Bar]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Disc Type Filter High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Double Coil Control Solenoid Valve of Butterfly Valve]

3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Reverse Flow Configuration Axial Fan-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Small Paper Gift Box with Magnet Closure for E-Cigarette]

3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension]

3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Handcraft Wood Jewelry Storage Box with Lock and Key, Part I.TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS]

Single Spindle Micro CNC Lathe Ck0640, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring.Pipe Thermostat with Sensor Ce Certificate, 175-184[30w Raycus Fiber Laser Marking Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone]

Whitaker,J.R.(1963),High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line. Analytical Chemistry35(12), 1950-1953[High Quality Male Water Quick Release Coupling]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA ApprovedAuthor for correspondence