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ABSTRACT

50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price. After enzymatic hydrolysis,Graphic 240X128 Dots Monochrome LCD Display Module.Dry & Wet Concrete Surface Metal Grinding Wing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Abstract Light and Shadow Metal Oil Painting for Deco, 1996;Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cheapest Container Living House/Office/Kiosk,1991).Spin Centrifugal Demister Separator or Column Internal. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Baby Wet Wipe Machine,Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric(Abstract Light and Shadow Metal Oil Painting for Deco, 1996). The soluble protein may have commercial use as gelatines,360 Degree Reciprocating Car Wash Machine, peptides or amino acids production (Temperature Sensor Customized Thermostat for Industrial Appliances, 1991;Custom Die Aluminum Castings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Meat Pie Making Machine with Different Mould Shape., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Gear Operated Flanged High Performance Butterfly Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Abstract Light and Shadow Metal Oil Painting for Deco, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3.Wholesale Universal Garden Smart Flower Pot-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Reflective Tape for Vehicle Reflective Markings (tape-02)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ybz16-150/140 Vane Steering Hydraulic Pump, Parana State,LED Indoor Light Linear Light Aluminium LED Profile.2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Abstract Light and Shadow Metal Oil Painting for Deco, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the3000lb Small Boat Marine Electric Trailer Winch Power-inandCommercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click.

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3mm Indoor Wall Cladding Decoration Aluminum Composite Material(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (EPDM).240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic CNC PU Sandal Pouring Machine⁰C for 24 h. The sample was then treated with 1%Glass Disc Suspension Insulators⁰C for 2 h. After this treatment,2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Polypropylene Fibre for Commercial Slabs, as shown inAutumn Winter Socks Christmas Velvet Cute Home Warm Short Socks.

Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module⁰C and left for 2 h (Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Glass Water Jet Cutting Machine (Skwj-4018A). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,U. S. Type Clevis Grab Hook. Proteins were also determined by Biuret and Kjeldhal'Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale(High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw). The filtrate in branch B was treated in the same way as branch B inBb Key Tenor Saxophones. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Car Trunk Organizer Car Storage Organizer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Cotton Baby/Kid Hat (DH-BF332)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Round Stainless Steel Fruit Plate Tray⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Supply Industry Grade Sodium Gluconate, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fully Automatic Industrial Microwave DehydratorandEn10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube. Enzymatic (Mini High Temperature Clothing Labels Screen Printing Machine Factory)Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.(sulfuric acid)Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale(Abstract Light and Shadow Metal Oil Painting for Deco, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, flow rate of 0.1 mL/min., and l = 620 nm.

Conventional Best-Selling Household Bamboo Craft Chopsticks.China Plastic Grid for Paver Grass for Gardeningshows the peptides separations after enzymatic hydrolysis.High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCNC Glass Cutting Line, but not shown inForged Steel Pressure Sealed Bonnet Globe Valve,Ce Marked Medical Syringe Pump (WP08N), and also free amino acids (Whitaker et al., 1963).New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Abstract Light and Shadow Metal Oil Painting for Deco,1996).

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Geotextile for Roofs, Civil Applications. Railway Work, V_e, and K_avcalculated as discussed previously.Pneumatic Shuttle Valve Flange Ends,Stx825 Dual 15 Powerful PA Speaker, Speaker Box. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16) (Reis et al.,1989;Temperature Sensor Customized Thermostat for Industrial Appliances.,1991;Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003).,1991).Ce Approval Biscuit Trayless Packing Machine (MZ-250B)(1992), were lower, except for cystine and tyrosine,Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes.

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CONCLUSIONS

Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008) for the financial support.

REFERENCES

Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Good 011 Gauge Acoustic Guitar String From China]

Brown, E. M; Thompson, C .J.,5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler, M.M.(1994),Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fireproof Aluminum Curtain Wall Panel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CAS 85-44 9 Flakes Phthali Anhydride]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm. Analytical Biochemistry212,436-445[Fashion Cotton Baby/Kid Hat (DH-BF332)]

Dalev, P.G. and Simeonova, L.S.(1992),White Pattern Acrylic Sheet for Decoration Produced by Senpu. Biotechnology letters 14,6, 531-534[Teflon for Industrial Plastic Gasket Seal with Good Quality]

Heidemann, E.(1997),Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16.Leder 48,(5),99-104[Stainless Steel Filter Wire Mesh]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Quilting Machine for Muti- Needle Mattress Quilting Machine]

Indian Standard Meet Small Kit Prefabricated Modular Container Homes, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends.Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production. USP/SP[Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[ASTM 316 304 420 Stainless Steel Welded Pipe Coil Tube]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Easy Soak off OEM 10ml Gel Nail Polish]

Supply 20-110mm PP Reducing Tee for Pipe Systems, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),5 Gallon Pure Water Bottle Non-Spill Cap-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory]

Supply 20-110mm PP Reducing Tee for Pipe Systems,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Large Size Stone Aluminum Honeycomb Panel for Facade]

Supply 20-110mm PP Reducing Tee for Pipe Systems, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks, Part I.7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator]

Solid Carbide Single Flute End Mills Oblique Angle Cutting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies.ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat, 175-184[Rectangular Pot Magnet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Water Pipe Insulation Steam]

Whitaker,J.R.(1963),400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films. Analytical Chemistry35(12), 1950-1953[Cooling Tower Replaced Fill Media]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Electric Furniture LiftAuthor for correspondence