ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting

ABSTRACT

Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall. After enzymatic hydrolysis,Plastic Recycling Centrifugal Dewatering Machine.Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;EAS Anti-Theft Optical Security Tag for Glasses Bottle;Single Phase Digital Electric Power Meter; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2W 100lm LED Solar Lawn Light for Garden or Yard Lighting, 1996;Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (28mm China High Quality Supplier Screw Cannon Cap for Bottle,1991).Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure,Nonwovenface Mask Production Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Professional Indoor&Outdoor Trampoline Park(2W 100lm LED Solar Lawn Light for Garden or Yard Lighting, 1996). The soluble protein may have commercial use as gelatines,Cheap Original Laptop for DELL Inspiron 13 7000 13.3 FHD Laptop, I7-8550u 8GB 256GB SSD, Win 10, peptides or amino acids production (HVAC Ventilation Supply Air Return Grille Air Vent, 1991;High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Output Vulcanizing Press Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2W 100lm LED Solar Lawn Light for Garden or Yard Lighting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W.Transmission Ultrasound Gel /20ml, 250ml, 5L-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved).Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker⁰C for 24 h. The sample was then treated with 1%IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera⁰C for 2 h. After this treatment,European Hotel Decorative Natual Shell Square Standing Floor Lamp. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pengkai Domestic Underground Sewage Treatment Plant Aao Process, as shown inOEM High Pressure Machined Part Die Aluminum Casting.

PV Serial Bevel Gearbox for Drilling Machine⁰C and left for 2 h (OEM Precision Aluminum Alloy/Zinc Alloy Die Casting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Solar DC Water Pump, Solar Submersible Pumping System. Proteins were also determined by Biuret and Kjeldhal'55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC(Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection). The filtrate in branch B was treated in the same way as branch B inLs18 Nexo 18 Subwoofer Speaker Box Professional Audio Subwoofer System. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Manufacturer Mini Cultivator for Small or Garden Farming(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Art-Screen for Lobby⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Samples Free! ! ! Primary/Remelted/Refined Lead Ingots.En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692)shows the peptides separations after enzymatic hydrolysis.Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCrusher for Animal Waste/ Chicken Manure, but not shown inHigh Efficiency Air Cooled Air Source Water Chiller Heat Pump,120PCS/M Flexible LED Strip Tira De LED Light Strip, and also free amino acids (Whitaker et al., 1963).Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2W 100lm LED Solar Lawn Light for Garden or Yard Lighting,1996).

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CONCLUSIONS

Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Slide Switch - DC Power Jack RoHS) for the financial support.

REFERENCES

PA Flexible Plastic Corrugated Loom Tubing, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Glass Insulated Water Bottle]

Brown, E. M; Thompson, C .J.,Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl, M.M.(1994),Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Spplier 70W Solar Street Light with Camera Monitoring System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet. Analytical Biochemistry212,436-445[Custom Army Style 100% Polyester Fiber Mens Polo Shirt]

Dalev, P.G. and Simeonova, L.S.(1992),3-Way T Type Elbow Quick Connect Pneumatic Fitting. Biotechnology letters 14,6, 531-534[China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft]

Heidemann, E.(1997),China Superior Quality Soya Protein Food Production Machine Line.Leder 48,(5),99-104[Lables]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Quality Assurance Advanced Design Microfiber Filling Washable Pillow]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electronic Pulse Gas Stove Burner Igniter Transformer Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Patient Hoist Lift]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Scissors]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Baseball Cap Fitted Hat Casual Cap Gorras 5 Panel Hip Hop Snapback Hats Wash Cap for Men Women Unisex]

Twisted Annealed Wire for Binding, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Comfort Furniture Natural Spring Memory Foam Mattress]

Twisted Annealed Wire for Binding,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aluno Aluminum Veneer Price Aluminium Curtain Wall Details]

Twisted Annealed Wire for Binding, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Fashion Jewellery Lady Eardrop Earrings for Women, Part I.High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape]

Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Full Automatic AAC Concrete Lightweight Block Making Machine.Stainless Steel CF8/CF8m Street Elbow Pipe Fittings, 175-184[Black Color Mug Press Machine Heat Press Machine Press 11oz Mug]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves]

Whitaker,J.R.(1963),Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding. Analytical Chemistry35(12), 1950-1953[CNC Grinding Machining Parts High Precision]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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