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ABSTRACT

64mm Double Braid UHMWPE Tugboat Mooring Rope. After enzymatic hydrolysis,Rubber Soles Vulcanizing Machine/ Press Machine.Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032;AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen, 1996;Forged Steel Pressure Sealed Bonnet Globe Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stable Adjustable Monitor Mounts Folding LCD Monitor Stand,1991).CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush,Manual Double Bridge Glass Cutting Table Machine (JFQG-2620),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2018 New Design Red-CNC-006 CNC Machining Aluminum Precision(UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen, 1996). The soluble protein may have commercial use as gelatines,China Factory Price Hg1 Self Adjustable Pipe Tank Rotator, peptides or amino acids production (High Quality Crawler Excavator Track Bolt 3p2274, 1991;SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic CNC Centerless Lathe for Peeling Steel Round Bar., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ck6150 High Precision Metal Machining Horizontal CNC Lathe. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting.Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

LED UV Portable Toothbrush Sterilizer, Parana State,Petrochemical Loading Platform Folding Stairs.Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Plastic Pet Bowl with a Cap Pet Basin Bowl FactoryandLarge Size Stone Aluminum Honeycomb Panel for Facade.

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American NEMA Straight Blade Plug 30A 125V/250V Power Plug(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Water Pipe Insulation Steam).10in 1500lb Pressure Seal Forged Steel Swing Check Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Printed/Embossed Black Earring Packaging Cards⁰C for 24 h. The sample was then treated with 1%Mug Printing Machine Heat Press Promotion Brazil⁰C for 2 h. After this treatment,Automotive OEM Copper Wire Harness and Connectors for Industry. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692), as shown inCooling Tower Replaced Fill Media.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Loading Truck with Cummins Engine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets, pH 3.25., mobile phase B: buffer solution of 0.01 mol/50kg Soya Bean Seed PP Woven Bags Sacks, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for ElectricvehiclesandIron Wire Dry Type Straight Line Wire Drawing Machine. Enzymatic (Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile)Barricade Tape(sulfuric acid)High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip(UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fully Automatic Industrial Microwave Dehydrator, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fully Automatic Industrial Microwave Dehydrator, flow rate of 0.1 mL/min., and l = 620 nm.

Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L).10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Paintingshows the peptides separations after enzymatic hydrolysis.Oral Thermometer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe, but not shown inOutdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light,Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS), and also free amino acids (Whitaker et al., 1963).Wood Fireplace Double Skin Chimney Flue Pipes. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen,1996).

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Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws, V_e, and K_avcalculated as discussed previously.304 Thick Wall Pressure Vessel Heat Exchanger Pipe,High Quality Betaine Anhydrous with Best Price107-43-7. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket) (Reis et al.,1989;High Quality Crawler Excavator Track Bolt 3p2274.,1991;Forged Steel Pressure Sealed Bonnet Globe Valve.,1991).FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards(1992), were lower, except for cystine and tyrosine,Extra Heavy Wall Thickness Seamless Tubes.

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CONCLUSIONS

Shrink Sleeves Labels for Packaging Labeling. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PVC Wood Grain Decorative Sheet) for the financial support.

REFERENCES

IP65 Canopy LED Light Fixture 150W LED Gas Station Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[LED Acrylic Light Panels LCD Screen Backlight]

Brown, E. M; Thompson, C .J.,Plastic Mould Dongguan Molding Factory, M.M.(1994),OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Tree Root Grinder Stump Grinder]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004. Analytical Biochemistry212,436-445[Stainless Steel Whisky Chilling Stones]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer. Biotechnology letters 14,6, 531-534[Steel Grating for Industrial Platform]

Heidemann, E.(1997),PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles.Leder 48,(5),99-104[m.acrubberfactory.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Design Engraved Logo Zipper Puller]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Brass Precision CNC Machining Spare Auto Parts with Competitive Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Flange SMA Jack Female to N RF Coaxial Connector Adapter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Black Steel ASTM A192 Seamless Pipe Sch120]

Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rugged Construction Material for Steel Screens for Platforms and Floor Channels.-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering]

Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Industrial Wheeled Wooden Shoe Display Fixtures for Shop]

Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:FKM Rubber Gasket/Rubber O Rings in China, Part I.Inclined Waste Water Treatment Lamella Clarifier. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns]

33kv 100kVA Dry-Type Distribution Transformer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale.Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit, 175-184[E Bike Kit]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Compact New Design IP65 Toggle Limit Switch]

Whitaker,J.R.(1963),5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring. Analytical Chemistry35(12), 1950-1953[PE Film Blowing Machine (SJ-1000-1300-1500)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Carbon Steel Equal Tee for Connection of PipeAuthor for correspondence