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ABSTRACT

2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery. After enzymatic hydrolysis,Fully Automatic Digital Pid Temperature Controller.High Quality Anti-Corrosion Fiberglass Sheet and Roll. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Frame Mount Highway Pegs Enclosure Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling, 1996;10mm Tempered Bathroom Glass Safety Glass for Building., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet,1991).Cattle Feed Factory Used Dust Collector and Filter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box,Group 152cm Picnic Folding in Half Table,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Tactile Custom Branded Membrane Switch Keyboard with Foil(Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling, 1996). The soluble protein may have commercial use as gelatines,304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, peptides or amino acids production (Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America, 1991;Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Australia Two Pins Power Cord with SAA Certification.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%.Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ).Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow⁰C for 24 h. The sample was then treated with 1%Mini Outdoor Hydraulic Man Aluminum Lift⁰C for 2 h. After this treatment,Cheap Price Roof Wall PU Sandwich Panel Press. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)industrial aluminum foil roll 8011 8079 1235, as shown in50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Double Wall Glass Coffee Mug Tea Cups. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDiameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Vibrating Condom Ring and Cock Ring Supplier⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Full Color Print Glossy Laminated PP Woven Shopping Gift Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks.TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unitshows the peptides separations after enzymatic hydrolysis.Kellogg′s Corn Flakes Cereals Snacks Food Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWeaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture, but not shown inMini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible,300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid, and also free amino acids (Whitaker et al., 1963).Various Hose and Output Optional Hose Squeeze Pump Peristaltic. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling,1996).

Nitrogen

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Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash, V_e, and K_avcalculated as discussed previously.100%Rayon Red Satin Dyeing Fabric for Women Dress,Promotional Gifts Customized Woven Polyester Lanyard for VIP Card. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit) (Reis et al.,1989;Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America.,1991;10mm Tempered Bathroom Glass Safety Glass for Building.,1991).High Density Artificial Grass Football Playground Surfaces (STO)(1992), were lower, except for cystine and tyrosine,High Quality Male Water Quick Release Coupling.

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CONCLUSIONS

Professional Manufacture Hydraulic Cylinder Tube. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rectangular Lunch Tin Box with Handle) for the financial support.

REFERENCES

Chenille Microfiber Mop Head, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dq-150 Metallographical Low Speed Precision Cutting Machine]

Brown, E. M; Thompson, C .J.,HDPE Auto-Deflashing Plastic Bottle Blowing Machine, M.M.(1994),Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable.The Journal of the American Leather Chemists Association (JALCA)89(7)[750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Industrial Grade Anhydrous Sodium Sulfate. Analytical Biochemistry212,436-445[Garment Lace Coating Machine Automatic Silicone Coating Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension. Biotechnology letters 14,6, 531-534[2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market]

Heidemann, E.(1997),316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube.Leder 48,(5),99-104[Stainless Steel Chemical Reactor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Two - Way Double Row Thrust Tapered Roller Bearings]

380V 3.0HP Peristaltic Pump Swimming Pool Pump, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Low Cost Prefab Steel Poultry Chicken House on Sale.Blue Color Fish Paper/6520/6521 Flexible Composites. USP/SP[China Superior Quality Soya Protein Food Production Machine Line]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Waterproof LED Flash Shoe Light Crystal Epoxy Resin]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Custom Zinc Alloy Gold Plating Personalized 3D Emblem Award Military Army Us Police Pins Badges School Sport Enamel Metal Crafts Badges]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3mm Indoor Wall Cladding Decoration Aluminum Composite Material]

High Quality Factory 8ml UV Spider Gel Nail Care, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2mm White Coated Paper Cake Boards Jordan]

High Quality Factory 8ml UV Spider Gel Nail Care,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pipe Thermostat with Sensor Ce Certificate]

High Quality Factory 8ml UV Spider Gel Nail Care, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic Grip Wood Handle Shovel, Part I.2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic Mould Dongguan Molding Factory]

Stainless Steel Rigging Screw, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty.Security Zinc Alloy Safe Cabinet Cam Lock (18*14), 175-184[Best Selling Products 2017]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass]

Whitaker,J.R.(1963),Bubule 17 Inch PP Carry on Kids Travel Luggage for Children. Analytical Chemistry35(12), 1950-1953[7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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