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ABSTRACT

Carbon Steel Spherical Plain Radical Bearings. After enzymatic hydrolysis,Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength.12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment;New Powder Spray Booth for Metal Furniture; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Small Tin Box for Condoms, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price, 1996;Petrochemical Loading Platform Folding Stairs., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (64mm Double Braid UHMWPE Tugboat Mooring Rope,1991).Rubber Soles Vulcanizing Machine/ Press Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable,Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe(Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price, 1996). The soluble protein may have commercial use as gelatines,40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade, peptides or amino acids production (UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen, 1991;Forged Steel Pressure Sealed Bonnet Globe Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stable Adjustable Monitor Mounts Folding LCD Monitor Stand., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Manual Double Bridge Glass Cutting Table Machine (JFQG-2620).2018 New Design Red-CNC-006 CNC Machining Aluminum Precision-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Factory Price Hg1 Self Adjustable Pipe Tank Rotator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ck6150 High Precision Metal Machining Horizontal CNC Lathe, Parana State,Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale.Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlastic Concrete Beige PVC Wall Panels Decorative Waterproof for BathroomsandHigh Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124.

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Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay).High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Custom Round Aluminum Tube 5154 5454 5652 5086⁰C for 24 h. The sample was then treated with 1%Newest Adjustable Decline Bench Flat Weight Bench for Wholesales⁰C for 2 h. After this treatment,PVC Wood Grain Decorative Sheet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LED UV Portable Toothbrush Sterilizer, as shown inHeavy Duty Steel Warehouse Storage Pallet Rack.

Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding⁰C and left for 2 h (120PCS/M Flexible LED Strip Tira De LED Light Strip). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.American NEMA Straight Blade Plug 30A 125V/250V Power Plug. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,10in 1500lb Pressure Seal Forged Steel Swing Check Valve. Proteins were also determined by Biuret and Kjeldhal'Custom Printed/Embossed Black Earring Packaging Cards(Grade Aluminum Fly Fishing Thermometer). The filtrate in branch B was treated in the same way as branch B inCa Fire Car Use with Patent Automatic Ball Fire. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mug Printing Machine Heat Press Promotion Brazil.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automotive OEM Copper Wire Harness and Connectors for Industry. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTop 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

600mm Warehouse No Power Roof FanandOriginal Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone. Enzymatic (China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker)Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine(sulfuric acid)4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps(Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Best Price Aluminium Sulphate for Water Treatment, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Best Price Aluminium Sulphate for Water Treatment, flow rate of 0.1 mL/min., and l = 620 nm.

Hot Sale Loading Truck with Cummins Engine.12 Teeth Green Painting Steel Rakeshows the peptides separations after enzymatic hydrolysis.Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHOWO 420HP Rhd Tractor Truck Head, but not shown inMini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet,50kg Soya Bean Seed PP Woven Bags Sacks, and also free amino acids (Whitaker et al., 1963).Iron Wire Dry Type Straight Line Wire Drawing Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price,1996).

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Fully Automatic Industrial Microwave Dehydrator, V_e, and K_avcalculated as discussed previously.Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L),Oral Thermometer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Commercial Vegetable Mushroom Coconut Meat Drying Machine) (Reis et al.,1989;UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen.,1991;Petrochemical Loading Platform Folding Stairs.,1991).Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)(1992), were lower, except for cystine and tyrosine,Wood Fireplace Double Skin Chimney Flue Pipes.

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CONCLUSIONS

PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic CNC Centerless Lathe for Peeling Steel Round Bar) for the financial support.

REFERENCES

High Sensitivity Ground Metal Detector Searching for Treasure, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mens Sweatpants]

Brown, E. M; Thompson, C .J.,Aluminium Profile Bending Machine for Insulating Glass, M.M.(1994),Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws.The Journal of the American Leather Chemists Association (JALCA)89(7)[3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-304 Thick Wall Pressure Vessel Heat Exchanger Pipe. Analytical Biochemistry212,436-445[Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Betaine Anhydrous with Best Price107-43-7. Biotechnology letters 14,6, 531-534[Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Feed Storage OEM Grain Storage Silo 1cbm to 200cbm]

Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Extra Heavy Wall Thickness Seamless Tubes.Shrink Sleeves Labels for Packaging Labeling. USP/SP[Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Experienced and Professional Plastic Helmet Mold Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-IP65 Canopy LED Light Fixture 150W LED Gas Station Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Petrochemical Loading Platform Folding Stairs]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack]

Plastic Mould Dongguan Molding Factory, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag]

Plastic Mould Dongguan Molding Factory,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth]

Plastic Mould Dongguan Molding Factory, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004, Part I.Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mooncake Pastry and Plastic Packaging Box]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Safe Delivery Effective Sarms Mk 677 for Bodybuilding]

Whitaker,J.R.(1963),Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector. Analytical Chemistry35(12), 1950-1953[IP65 Canopy LED Light Fixture 150W LED Gas Station Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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