ARTICLES

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ABSTRACT

J55 Short Round Thread Seamless Casing Pipe. After enzymatic hydrolysis,2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine.3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dust Hood Fume Extraction Systems for Robotic Laser Cutting, 1996;IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nordic Solid Wood Dining Chair/Smile Chair,1991).Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Experienced and Professional Plastic Helmet Mold Manufacturer,Private Label OEM Best Cute Waterproof Lip Pluming Gloss,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display(Dust Hood Fume Extraction Systems for Robotic Laser Cutting, 1996). The soluble protein may have commercial use as gelatines,Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press, peptides or amino acids production (High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine, 1991;IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Tent Windows Use China Made PVC Transparent Clear Mesh Tarp., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.13 Oz Flex Banner PVC Frontlit Banner Material.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dust Hood Fume Extraction Systems for Robotic Laser Cutting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables.Chubby 163cm Fat Big Ass Female Real Sex Doll for Man-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Drive Travel Car Inflatable Bed(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se).Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China PCBA Supplier Custom Game Controller PCB⁰C for 24 h. The sample was then treated with 1%TPU TPR Rubber Band Plastic Extruder Machine⁰C for 2 h. After this treatment,Wheel Loader Transmission Gear Box/Customized Casting Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Auto Spare Parts Ignition Coil 22448-2y001, as shown inWicker Flower Baskets for Planter.

3D Good Quality Laser Engraving Machine for Souvenirs Shop⁰C and left for 2 h (Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording. Proteins were also determined by Biuret and Kjeldhal'Reverse Flow Configuration Axial Fan(Indian Standard Meet Small Kit Prefabricated Modular Container Homes). The filtrate in branch B was treated in the same way as branch B inPrefabricated Safety Garden Steel Iron Wire Mesh Fence. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Handcraft Wood Jewelry Storage Box with Lock and Key.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSingle Spindle Micro CNC Lathe Ck0640(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pipe Thermostat with Sensor Ce Certificate⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle StrawandNitinol Disposable Guide Wire. Enzymatic (Breathable Surgical Paper Tape/ Micropore Medical Tape)Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow(sulfuric acid)Crane Hook Qtz250 (7032) with 70m Jib Length(Dust Hood Fume Extraction Systems for Robotic Laser Cutting, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Investment Precision Casting for Valve Body with Machining) for the financial support.

REFERENCES

Outdoor High Neck Kids Boots Casual Rubber Men Work Boots, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Timber Window Shutter Profile Facotry Gesso Primer Window Shuter Louver Components Manufacturer]

Brown, E. M; Thompson, C .J.,Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061), M.M.(1994),Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector.The Journal of the American Leather Chemists Association (JALCA)89(7)[2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet. Analytical Biochemistry212,436-445[Men′s High Quality Mercerised Cotton Business Dress Socks]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Grinding Machining Parts High Precision. Biotechnology letters 14,6, 531-534[900kg Pneumatic Motorcycle Lift]

Heidemann, E.(1997),High Quality Cheap Price Disposable IV Infusion Set with Precise Filter.Leder 48,(5),99-104[Dental Clinic]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mug Printing Machine Heat Press Promotion Brazil]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2W 100lm LED Solar Lawn Light for Garden or Yard Lighting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Disposable Clear Take Away Plastic Salad Bowl Forming Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fabric Corrosion-Resistant Pultrusion FRP/GRP Round Pipe (GH G003)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter]

Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Colorful Electric English Funny Music Story Module Printing Book]

Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ybz16-150/140 Vane Steering Hydraulic Pump]

Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge, Part I.Carbon Stee Semless Pipe (ASTM A106 Gr B). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment]

OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent.Hot Selling House Floor Cleaning 360 Spin Mop, 175-184[3d Laser Cutting Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Farm Machinery Auto Mechanical Straw Crusher for Poultry]

Whitaker,J.R.(1963),Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles. Analytical Chemistry35(12), 1950-1953[Pet Short Fiber Non Woven Geotextile for Landfill Filtration]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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