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ABSTRACT

White Pattern Acrylic Sheet for Decoration Produced by Senpu. After enzymatic hydrolysis,Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16.SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Supply 20-110mm PP Reducing Tee for Pipe Systems, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (5 Gallon Pure Water Bottle Non-Spill Cap, 1996;Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care,1991).Solid Carbide Single Flute End Mills Oblique Angle Cutting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies,ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films(5 Gallon Pure Water Bottle Non-Spill Cap, 1996). The soluble protein may have commercial use as gelatines,Small Capacity Automatic Cup Filling Machine, peptides or amino acids production (Seasoned Cast Iron South Africa Three Legged Potjie Pot, 1991;Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 New Release Doypack Sachet Powder Packing Machine Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Amplifier Tube Glass Vacuum Tube (833C). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (5 Gallon Pure Water Bottle Non-Spill Cap, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools.New Style Custom Logo Makeup Multi Colored Eyeshadow Palette-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway).Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Industrial Wheeled Wooden Shoe Display Fixtures for Shop⁰C for 24 h. The sample was then treated with 1%Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness⁰C for 2 h. After this treatment,Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Aluminum Carpet Topping Stage Platform, as shown in110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator.

BY60100C type metal shaping machine/hydraulic shaper machine⁰C and left for 2 h (New Design Many Styles Fancy Wine Display Rack (6532)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Spplier 70W Solar Street Light with Camera Monitoring System. Proteins were also determined by Biuret and Kjeldhal'Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud(PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer). The filtrate in branch B was treated in the same way as branch B inHigh Quality Mens Nightwear Hospital Pajamas Ladies Pajamas. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avExcavated Caterpillar Loader Mining Tunnel Loader Mucking Loader(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Masturbators Adult Sex Toys Japanese Love Doll, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Small Tin Box for Condoms.4 Gang 1 Way Electrical Fitting Ce Certificateshows the peptides separations after enzymatic hydrolysis.Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale High Speed Mixer Blender for Extruder Machine, but not shown inInfant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old,Petrochemical Loading Platform Folding Stairs, and also free amino acids (Whitaker et al., 1963).64mm Double Braid UHMWPE Tugboat Mooring Rope. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (5 Gallon Pure Water Bottle Non-Spill Cap,1996).

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AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe, V_e, and K_avcalculated as discussed previously.40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade,UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Maleic Anhydride CAS 108-31-6) (Reis et al.,1989;Seasoned Cast Iron South Africa Three Legged Potjie Pot.,1991;Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks.,1991).Forged Steel Pressure Sealed Bonnet Globe Valve(1992), were lower, except for cystine and tyrosine,Stable Adjustable Monitor Mounts Folding LCD Monitor Stand.

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CONCLUSIONS

CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume) for the financial support.

REFERENCES

Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM Customized Aluminum Alloy Helical Gear Reducer Planetary Transmission Gearbox]

Brown, E. M; Thompson, C .J.,Manual Double Bridge Glass Cutting Table Machine (JFQG-2620), M.M.(1994),2018 New Design Red-CNC-006 CNC Machining Aluminum Precision.The Journal of the American Leather Chemists Association (JALCA)89(7)[Replica Car Wheels with 20inch for BMW Car]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Factory Price Hg1 Self Adjustable Pipe Tank Rotator. Analytical Biochemistry212,436-445[ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Good Design Custom Made Cheap Recycle Paper Bags]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ck6150 High Precision Metal Machining Horizontal CNC Lathe: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized UV Drying Heating Machine for Screen Printer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metamax / Meta Aramid / Nomex Dust Collector Bags]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15)]

Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip]

Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ PVC Butterfly Valve Gear Type]

Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, Part I.Factory Custom Round Aluminum Tube 5154 5454 5652 5086. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Drive Travel Car Inflatable Bed]

Whitaker,J.R.(1963),LED UV Portable Toothbrush Sterilizer. Analytical Chemistry35(12), 1950-1953[1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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